3D printing has been widely focused on as a promising manufacturing technology in various industries due to its technical flexibility. In particular, in the biomedical field, the advances in such technology have opened up great chances to improve the quality of patients’ life and precision medicine-related healthcare. In this special issue, biomedical devices primarily include human tissue models/chips, biosensors, bioelectronics, and so on. This issue is especially interested in the trends of 3D printing-based approaches and strategies for creating such devices, but not limited to 3D printing.