AccScience Publishing / IJB / Online First / DOI: 10.36922/ijb.3258
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RESEARCH ARTICLE

3D printing collagen type IV for corneal endothelium transplantation

Morgan Overmass1,2 ,  Huasheng Huang1,2 ,  Li Wen2,3 ,  Zhi Chen4 ,  Sepidar Sayyar4,5 ,  Constantinos Petsoglou1,3 ,  Gordon Wallace4,5 ,  Gerard Sutton2,3,6 ,  Jingjing You1,2*
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1 School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, Australia
2 Save Sight Institute, Sydney Medical School, University of Sydney, Sydney, Australia
3 NSW Tissue Bank, Sydney, Australia
4 ARC Centre of Excellence for Electromaterial Science, Intelligent Polymer Research Institute, AIIM, Innovation Campus, University of Wollongong, Wollongong, Australia
5 Australian National Fabrication Facility – Materials Node, Innovation Campus, University of Wollongong, Wollongong, Australia
6 Vision Eye Institute, Chatswood, New South Wales, Australia
IJB 2024, 10(4), 3258 https://doi.org/10.36922/ijb.3258
Submitted: 25 March 2024 | Accepted: 9 May 2024 | Published: 21 June 2024
(This article belongs to the Special Issue Biomimetic and Bioinspired Printed Structures)
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Collagen type IV (Col-IV) is a critical matrix protein component in the basement membrane. In the cornea, it is the key contributor to the formation of Descemet’s membrane — the layer for corneal endothelial cells to reside on. Currently, endothelial keratopathy, which uses the healthy donor corneal endothelium layer as a transplanted tissue, is the gold standard surgery to cure corneal blindness caused by endothelial dysfunction. However, its use is being limited by the shortage of donor corneas. Bioengineering Descemet’s membrane is an alternative to overcome the tissue shortage. In this study, we developed a printable Col-IV ink to construct a Col-IV membrane. We demonstrated that the printed Col-IV membrane has a seven times higher corneal endothelial cell attachment over other matrix proteins (e.g., Col-I, laminin, and chondroitin) and a significantly faster healing rate compared to Col-I. The human endothelial cells cultured on the Col-IV membrane retained their hexagonal shapes, expressed typical endothelial markers ZO-1 and Na+/K+-ATPase, and retained the ability to deposit laminin. As this collagen subtype is prevalent in the basement membranes of all body tissues, our work suggests an important role for Col-IV as a printable biomaterial in tissue engineering in general.

Keywords
Collagen type IV
Bioprinting
Corneal endothelium
Basement mem¬brane
Printable biomaterial
Endothelium transplant
Funding
We acknowledge the funding support from the NHMRC Idea Grant (GNT1181415), Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012), and Sydney Eye Hospital Foundation.
Conflict of interest
The authors declare the following financial interests/ personal relationships, which may be considered as potential competing interests: the printable Col-IV biomaterial (PCT/AU2022/050247) was also patented with M.O., G.W., G.S., and J.Y. being inventors. The bioengineered endothelium construction was patented (PCT/AU2022/050248) with M.O., S.H., L.W., C.P., G.S., and J.Y. being inventors.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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