This special issue will discuss significant contributions dealing with the design, synthesis and optimisation of bioinks for bioprinting, bioprinted structures and off-the-shelf implants, and their pre-clinical validation in multiple tissue regeneration applications. Bioprinting has tended to focus on the technology itself rather than on materials development and therefore there are many technical opportunities to move these printed materials/inks to higher performance levels. Current deposition and fabrication technologies allow researchers to design and build structures with increasingly intricate architectures. However, in achieving this, many concessions are being made regarding the biological aspects of printed materials. Therefore, there is a pressing need for novel engineering approaches to obtain biomimetic and bioinspired inks for tissue engineering and regenerative medicine.