Diarrheal diseases, particularly those caused by rotaviruses, pose a significant health threat, especially among children, and cause huge economic losses to the pig industry in the form of high morbidity, mortality, and stunted growth. Rotavirus A (RVA) remains the predominant viral agent for severe diarrheal episodes, contributing to high hospitalization and mortality rates in India. RVA’s high genetic diversity is attributed to frequent reassortment and mutations. This study aims to characterize the VP4, VP6, VP7, and NSP4 genes of RVA in stool samples collected from children and piglets in and around Bareilly, Uttar Pradesh, India. A total of 300 samples, including 100 from children and 200 from piglets, were screened for the detection of double-stranded RNA of RVA using ribonucleic acid-polyacrylamide gel electrophoresis (RNA-PAGE) and reverse transcription polymerase chain reaction (RT-PCR). Results revealed the RVA incidence, particularly in winter (end of November to beginning of February), aligning with observed seasonal trends. Among the 32 Rotavirus (RV)-positive samples from children, 21 (65.63%) were detected by RNA-PAGE, whereas 28 (87.5%) were identified by RT-PCR. Whereas, of the 80 RT-PCR positive samples from piglets, only 51 (63.75%) were detected by RNA-PAGE, indicating the superiority of RT-PCR. Molecular analysis identified the prevalent genotypes in human strains as G1, G2, G3, and P[8], whereas G9P[13]-I5-E1 dominated among piglets in a single farm outbreak. The findings underscore the critical need for continuous surveillance to monitor evolving RV genotypes from both humans and piglets, enabling the identification of new strains of RVA and subsequent modification of vaccination strategies to reduce RVA’s impact in India.