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Challenges and perspectives of liver tissue engineering: From cell therapy to bioprinting

Julio Rodríguez-Fernández1 ,  M. Teresa Donato2,3,4 ,  Gloria Gallego-Ferrer1,5* ,  Laia Tolosa2,5*
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1 Center for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Valencia, Spain
2 Experimental Hepatology Unit, Health Research Institute La Fe (IISLAFE), Valencia, Spain
3 Departament of Biochemistry and Molecular Biology, Faculty of Medicine, Universitat de València, Valencia, Spain
4 Biomedical Research Networking Center on Hepatic and Digestive Diseases (CIBER-EHD), Valencia, Spain
5 Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valencia, Spain
IJB, 2706 https://doi.org/10.36922/ijb.2706
Submitted: 10 January 2024 | Accepted: 26 February 2024 | Published: 29 March 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Liver tissue engineering offers a remarkable tool for toxicological screening and an approach to disease modeling, and provides an alternative solution to liver transplantation in treating end-stage liver diseases. As the liver is a highly specialized multicellular organ, distinctive features such as diversity of cell types, extracellular matrix composition, and three-dimensional architecture must be taken into consideration in recapitulating its physiology. Here, we review the current developments in liver tissue engineering approaches, focusing on their application to liver disease modeling and treatment, with special emphasis on bioprinting for high-throughput analysis. We also discuss the challenges encountered and the likely future developments in the area.

Keywords
Liver
Bioprinting
Disease modelling
Hepatotoxicity
Regenerative medicine
Funding
This work was funded by the Institute of Health Carlos III (ISCIII, Plan Estatal de I+D+i 2013-2016) and co-financed by the European Regional Development Fund “A way to achieve Europe” (FEDER) through Grant No. PI21/00223, and by Grants PID2022-136433OB-C21 and C-22 funded by MCIN/AEI/10.13039/501100011033 and Grant No. CNS2022-135425. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
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Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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