In this special issue original research articles, review articles and editorials are all very welcome. The state-of-the-art tissue engineering methods, tissue and disease models with different complexity, new developments in bioprinting technologies and equipments, furthermore evaluation and utilization of the innovative, 3D bioprinted artificial tissue models are in scope of this collection. The bioengineered tissue characterization with physical, mechanical, biochemical, immunological or imaging techniques, histological methods and functional tests can be presented. Manuscripts about the role and the current and future position of bioprinting technologies in the toolbox of preclinical research for drug development, and the possible therapeutic perspectives in e.g. transplantation medicine are also gladly accepted.