AccScience Publishing / IJB / Volume 9 / Issue 3 / DOI: 10.18063/ijb.686
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RESEARCH ARTICLE

The biological properties of 3D-printed degradable magnesium alloy WE43 porous scaffolds via the oxidative heat strategy

Shuyuan Min1,2† ,  Chaoxin Wang1,2 ,  Bingchuan Liu1,2 ,  Jinge Liu3 ,  Yu Liu1,2 ,  Zehao Jing1,2 ,  Yan Cheng4 ,  Peng Wen3* ,  Yufeng Zheng6 ,  Yun Tian1,2*
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1 Department of Orthopedics, Peking University Third Hospital, Beijing 100191, People’s Republic of China
2 Engineering Research Center of Bone and Joint Precision Medicine, Ministry of Education, Beijing 100191, People’s Republic of China
3 Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China
4 Biomed-X Center, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China
5 Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
6 School of Materials Science and Engineering, Peking University, Beijing, 100871, China
IJB 2023, 9(3), 686 https://doi.org/10.18063/ijb.686
Submitted: 11 August 2022 | Accepted: 8 November 2022 | Published: 15 February 2023
(This article belongs to the Special Issue Additive Manufacturing of Functional Biomaterials)
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

As a biodegradable material, magnesium alloy has a modulus similar to that of bone, and given the biological activity of its degradation products, it has the potential to be a bone grafting material. Oxidation heat treatment is a very effective passivation method that may reduce the rate of magnesium alloy degradation. Oxidation heat treatment increases the rare earth oxide content of the scaffold as well as the corrosion resistance of the scaffold. The overall cytotoxicity of the as-printed scaffolds (APSs) and oxidation heat-treated scaffolds (OHSs) showed that OHSs accelerated cell proliferation. In the apoptosis experiment, the OHS group had a cell survival rate between that of the control group and of the as-printed group. In the osteogenic induction experiment, the alkaline phosphatase activity and the quantity of mineralized nodules were greater in the APS and OHS groups than in the control group. Marker proteins for bone growth were expressed at higher levels in the APS and OHS groups than in the control group. Therefore, oxidation heat-treated 3D printing scaffolds with good biocompatibility and osteogenic properties have great potential to be made into advanced biomaterials that can be used to fix bone defects.

Keywords
Oxidation heat
3D printing
WE43 alloy
Magnesium alloy
Bone graft
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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