3D bioprinting technology is being used in various biomedical applications. These days, 3D bioprinting technology converged with nanotechnology is attracting more attention. In particular, as nanotechnology is applied to bioink used for 3D bioprinting, it is being used for various tissue engineering applications, and the cost reduction due to the increase in the utility of these two technologies is meeting various biomedical needs. This special issue aims to cover and provide an overview of these technologies and their integration. Topics include, but not limited to: bioactive nanomaterials, fabrication of complicated tissue, recapitulation of organ functions, modeling for 3D bioprinting.