During inkjet bioprinting, cells are subjected to shear stress directly while passing through the nozzles, which would cause reversible deformation on cell membranes. Moreover, other subcellular-level changes such as activation of gene pathways might also occur during inkjet bioprinting, leading to beneficial results. In this study, neural progenitor NE-4C cells were printed through 30μm thermal inkjet nozzles. Compared with manually pipetted cells (control group), a series of changes have occured on inkjet-bioprinted cells (inkjet group): Cell proliferation was down-regulated during the initial 4 days after bioprinting. Meanwhile, inkjet group exhibited stronger tolerance to high-concentration retinoic acid (RA). Most importantly, the expression level of early neuron marker tuj-1 was significantly higher in inkjet group, indicating the promotion of neuronal differentiation efficiency. Furthermore, RNA sequence and enrichment analysis was performed which had shown that cell-metabolism pathways were upregulated in inkjet group. These beneficial effects collectively suggested that inkjet bioprinting might be a promising strategy to accelerate neural tissue formation while further studies are performed.