AccScience Publishing / IJB / Online First / DOI: 10.36922/ijb.8357
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RESEARCH ARTICLE

Composite bacterial cellulose–psoralen– polycaprolactone scaffold for enhanced bone regeneration and infection prevention in open bone defects

Bo Jiang1 ,  Tianming Wang2 ,  Fengyong Mao1 ,  Xiao Zhao2 ,  Qingqiang Yao2 ,  Jiayi Li2* ,  Yang Huang3* ,  Jianchao Gui4*
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1 Department of Orthopedics, Children’s Hospital of Nanjing Medical University, Nanjing, China
2 Department of Orthopedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
3 International Innovation Center for Forest Chemicals & Materials and Jiangsu Co-Innovation Center of Efficient Processing & Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
4 Sports Medicine and Joint Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
IJB, 8357 https://doi.org/10.36922/ijb.8357
Submitted: 1 January 2025 | Accepted: 7 February 2025 | Published: 7 February 2025
© 2025 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

This study aimed to improve the treatment of open bone defects by developing a hybrid scaffold that enhances bone regeneration and prevents infections. Polycaprolactone (PCL) scaffolds were incorporated for mechanical support, while bacterial cellulose (BC) membranes facilitated psoralen (Pso) delivery. PCL scaffolds were fabricated via three-dimensional (3D) printing, and BC was extracted from fungi to prepare three types of scaffolds as follows: PCL, BC–PCL, and BC–Pso–PCL. The scaffolds were characterized through scanning electron microscopy, contact angle measurement, and infrared spectroscopy. Biocompatibility was assessed by cytotoxicity (CCK-8) assay, cell migration assay, live/dead cell staining, and cell proliferation experiments. Antibacterial effects were tested under a simulated bacterial environment. Osteogenic performance was evaluated by alkaline phosphatase (ALP) activity, Alizarin red staining (ARS), and immunofluorescence after osteogenic induction. Quantitative reverse transcriptase PCR (qRT-PCR) and Western blotting were performed to analyze bone regeneration and angiogenesis markers. Bone regeneration efficacy was assessed in vivo using a 5 mm critical-sized cranial bone defect model in rats. Biocompatibility studies demonstrated that all three scaffolds showed good biocompatibility. BC–Pso–PCL scaffold effectively inhibited Staphylococcus aureus growth. The ALP staining and ARS following osteogenic induction indicated that the BC–Pso–PCL group exhibited superior ALP activity and mineralized nodule formation compared to other groups. Subsequent immunofluorescence, qRT-PCR, and Western blot further confirmed the superior osteogenic performance of the BC–Pso–PCL scaffold. In vivo experiments demonstrated that the BC–Pso–PCL scaffold achieved the highest level of new bone formation in rat cranial defects. In conclusion, the BC–Pso–PCL scaffold demonstrated superior biocompatibility in cytotoxicity, cell migration, live/dead staining, and proliferation assays, while also promoting bone regeneration and angiogenesis. The in vivo study further confirmed its superior potential in bone formation. These findings highlight the BC–Pso– PCL hybrid scaffold as a promising implantable scaffold for treating open bone defects, with potential for future clinical applications.

Graphical abstract
Keywords
Composite scaffold
Bone infection
Bone regeneration
Drug delivery
Psoralen
Funding
This study was supported by the National Key Research and Development Program of China (2023YFB3813000), the Science and Technology Project of Jiangsu Province (BE2022718), Nanjing International Joint Research and Development Project (202201028), and the National Natural Science Foundation of China (82102567).
Conflict of interest
The authors declare they have no competing interests
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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