AccScience Publishing / IJB / Online First / DOI: 10.36922/ijb.5659
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RESEARCH ARTICLE

Prevascularized cell sheets enhance therapeutic effect of extracellular matrix scaffolds in a one-stage skin grafting rat model

Fei Zhou1,2 ,  Ning Liu1,3 ,  Shuying Chen1,2 ,  Yahui Xiong1,2 ,  Zhaoqiang Zhang4 ,  Lei Chen1, 2* ,  Yingbin Xu1,2*
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1 Department of Burn, Wound Repair & Reconstruction, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China
2 Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China
3 Department of the Second Plastic and Aesthetic Surgery, The First People’s Hospital of Foshan, Foshan, Guangdong, China
4 Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong, China
IJB, 5659 https://doi.org/10.36922/ijb.5659
Submitted: 28 October 2024 | Accepted: 20 December 2024 | Published: 20 December 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Dermal substitutes (DS) have been widely used in wound repair, but the low-survival rate of skin grafts with a DS remains an important challenge in regenerative medicine. This study aims to investigate the efficacy of combining prevascularized human mesenchymal stem cell sheets (PHCS) with DS in improving full-thickness wound healing. We used porcine dermis-derived decellularized extracellular matrix to construct an optimized 3D-printed DS, which exhibited the desired structure, good mechanical properties, and biocompatibility. Meanwhile, we constructed PHCS with a certain microstructure, which could enhance microvessel formation and maturation and upregulate the expression of angiogenic growth factors. An immunocompetent one-stage skin graft model in vivo demonstrated that the combination of PHCS and DS, along with split-thickness meshed skin autografting, could improve the repair effect of skin grafts, reduce graft fibrosis and later scar formation, and increase blood perfusion of skin grafts through early angiogenesis and rapid microvessel maturation. Our results suggest that the combination of PHCS and DS improves the repair effect of skin grafts through early angiogenesis for full-thickness skin defect repair.

Graphical abstract
Keywords
Angiogenesis
Decellularized extracellular matrix
Dermal substitutes
Prevascularized human mesenchymal stem cell sheets
Skin grafts
Funding
This study was supported by the Natural Science Foundation of Guangdong Province (2020A1515110694).
Conflict of interest
The authors declare they have no competing interests.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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