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RESEARCH ARTICLE

3D-Printed mesoporous bioglass/ polycaprolactone scaffolds induce macrophage polarization toward M2 phenotype and immunomodulates osteogenic differentiation of BMSCs

Weihua Huang1,2,3,4 ,  Shuai Huang1 ,  Xitao Linghu3 ,  Wei-Chih Chen1 ,  Yang Wang1 ,  Jingjie Li1 ,  Huinan Yin1 ,  Hang Zhang1 ,  Weikang Xu2,5,6* ,  Qingde Wa3*
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1 Department of Orthopaedic Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Haizhu District, Guangzhou, Guangdong, China
2 Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Jianghai Avenue Central, Haizhu District, Guangzhou, Guangdong, China
3 Department of Orthopaedic Surgery, the Second Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China
4 Department of Orthopaedic Surgery, The Affiliated Qingyuan Hospital (Qingyuan People’s Hospital), Guangzhou Medical University, Qingcheng District, Qingyuan, Guangdong, China
5 National Engineering Research Center for Healthcare Devices, Guangdong Provincial Key Laboratory of Medical Electronic Instruments and Materials, Guangdong Institute of Medical Instruments, Tianhe District, Guangzhou, Guangdong, China
6 Guangdong Chinese Medicine Intelligent Diagnosis and Treatment Engineering Technology Research center, Jianghai Avenue Central, Haizhu District, Guangzhou, Guangdong, China
IJB, 3551 https://doi.org/10.36922/ijb.3551
Submitted: 30 April 2024 | Accepted: 19 June 2024 | Published: 31 July 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Bioceramic composite polycaprolactone (PCL) scaffolds are widely used in bone defect repair studies. Among them, bioactive glass (BG) is considered an excellent bone-based repair material due to its unique inorganic amorphous structure, bioactivity, and osseointegration properties. However, the dense pores and low specific surface area of ordinary BGs and mesoporous BGs limit the mechanical properties and bioactivity of the overall scaffolds, and it is often necessary to increase the proportion of BGs to offset these shortcomings. Here, we prepared highly active dendritic mesoporous structured bioactive glass (MBG) with a high specific surface area (457.14 m2/g) and pore volume (1.38 cm3/g) by sol-gel method. PCL scaffolds containing different percentages of MBG were prepared by three-dimensional printing technology, and the physicochemical and immunomodulatory osteogenic properties were investigated. The results showed that the low-concentration MBG/ PCL scaffolds with 10% content (10MBG/PCL) possessed the highest compressive strength (about two times that of pure PCL scaffolds) and excellent in vitro immunomodulatory osteogenic properties. Finally, 10MBG/PCL was selected for further exploration to investigate the effects of different fiber diameters (F300, F500, F800) and pore sizes (P200, P500, P800) on the scaffolds performance. Ultimately, we found that the 10MBG/PCL scaffolds with fiber diameter and pore size of 500 μm had high osteogenic potential, significantly induced macrophage polarization toward the M2 phenotype, and downregulated the expression of inflammatory genes and that this group was the most capable of mediating macrophage polarization and thus inducing the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) to form an immune microenvironment conducive to osteogenesis. This study is a step forward in the exploration of the performance of BG composite PCL scaffolds and provides a new idea for the development of bone graft materials. 

Keywords
3D printing
Dendritic mesoporous structured bioactive glass
Macrophages
Bone marrow mesenchymal stem cells
Immunoregulation
Osteogenic differentiation
Funding
This research was supported by the National Natural Science Foundation of China (32000964, 82160577), the Guangdong Province Science and Technology Plan Project (2024A1515012265, 2020B1111560001, and 2022A1515140193), the Program for Science and Technology Project of Guizhou Province, Qiankehe Platform Talents (No. [2021] 5613), the Key Program for Science and Technology Project of Guizhou Province (No. ZK [2021] 007), and the GDAS’ Project of Science and Technology Development (2022GDASZH-2022020402-01, 2 0 2 2 G D A S Z H - 2 0 2 2 0 1 0 1 1 0 , a n d 2020GDASZH-2022030604-01).
Conflict of interest
The authors declare no conflict of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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