AccScience Publishing / IJB / Online First / DOI: 10.36922/ijb.2057
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RESEARCH ARTICLE

Bioprinting with adipose stem cells and hydrogel modified with bioactive glass

Krishna C.R. Kolan1 ,  Apurv Saxena2 ,  Bradley A. Bromet3 ,  Lesa B. Steen4 ,  August T. Bindbeutel1 ,  Julie A. Semon3* ,  Delbert E. Day4 ,  Ming C. Leu1
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1 Department of Mechanical and Aerospace Engineering, Missouri University of Science and Technology, Rolla, Missouri, United States of America
2 Department of Chemistry, Missouri University of Science and Technology, Rolla, Missouri, United States of America
3 Department of Biological Sciences, Missouri University of Science and Technology, Rolla, Missouri, United States of America
4 Department of Material Science and Engineering, Missouri University of Science and Technology, Rolla, Missouri, United States of America
IJB 2024, 10(2), 2057 https://doi.org/10.36922/ijb.2057
Submitted: 18 October 2023 | Accepted: 31 January 2024 | Published: 1 March 2024
© 2024 by the Author(s).. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Bioprinting research is focused on utilizing growth factors and multiple cell types to create clinically relevant three-dimensional (3D) tissue models using hydrogels. Rheological and biological challenges are two main factors that limit the creation of extrudable bioactive hydrogels. In this study, we investigate incorporation of fast dissolving and bioactive borate glass in different weight to volume percentages (0.075 to 0.6%) to alginate-gelatin (1:1) hydrogel to improve rheological properties and enable bioprinting with bioactive glass. The addition of glass improved the stiffness of the hydrogel. Human adipose-derived mesenchymal stem cells (ASCs) were uniformly mixed in this bioink at 1 × 106 cells/mL concentration, and spheroid specimens were cultured in both static and dynamic culture conditions. Grid-shaped scaffolds measuring ~18 × 18 × 1 mm3 were fabricated with the viable glass concentrations, and ASC viability was evaluated using Live/Dead assay. Despite immediate toxicity, an increased viability after 7 days with 0.15 w/v % or less borate glass content demonstrated the potential in utilizing highly resorbable calcium-releasing biomaterials such as bioactive glasses to modify hydrogels suitable for bioprinting cellularized 3D structures.

Keywords
Bioink
Bioactive glass
Ceramics
Adipose stem cells
Alginate
Gelatin
Funding
This research was funded by the Intelligent Systems Center and the Center for Biomedical Research at the Missouri University of Science and Technology
Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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