AccScience Publishing / IJB / Volume 9 / Issue 5 / DOI: 10.18063/ijb.759
Cite this article
143
Download
1643
Views
Journal Browser
Volume | Year
Issue
Search
News and Announcements
View All
REVIEW

Hydrogels for 3D bioprinting in tissue engineering and regenerative medicine: Current progress and challenges

Wenzhuo Fang1† Ming Yang1† Liyang Wang2† Wenyao Li2 Meng Liu1 Yangwang Jin1 Yuhui Wang1 Ranxing Yang1 Ying Wang1 Kaile Zhang1* Qiang Fu1*
Show Less
1 The Department of Urology, Affiliated Sixth People’s Hospital, Shanghai JiaoTong University, Shanghai 200233, China
2 School of Materials Science and Engineering, Shanghai University of Engineering Science, Shanghai 201620, China
Submitted: 30 December 2022 | Accepted: 30 March 2023 | Published: 23 May 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Three-dimensional (3D) bioprinting is a promising and innovative biomanufacturing technology, which can achieve precise position controlling of cells and extracellular matrix components, and further create complex and functional multi-cellular tissues or organs in a 3D environment. Bioink in the form of the cell-loaded hydrogel is most commonly used in bioprinting, and it is vital to the process of bioprinting. The bionic scaffold should possess suitable mechanical strength, biocompatibility, cell proliferation, survival, and other biological characteristics. The disadvantages of natural polymer hydrogel materials include poor mechanical properties as well as low printing performance and shape fidelity. Over the past years, a series of synthetic, modified, and nanocomposite hydrogels have been developed, which can interact through physical interactions, chemical covalent bond crosslinking, and bioconjugation reactions to change the characteristics to satisfy the requirements. In this review, a comprehensive summary is provided on recent research regarding the unique properties of hydrogel bioinks for bioprinting, with optimized methods and technologies highlighted, which have both high-value research significance and potential clinical applications. A critical analysis of the strengths and weaknesses of each hydrogel-based biomaterial ink is presented at the beginning or end of each section, alongside the latest improvement strategies employed by current researchers to address their respective shortcomings. Furthermore, we propose potential repair sites for each hydrogel-based ink based on their distinctive repair features, while reflecting on current research limitations. Finally, we synthesize and analyze expert opinions on the future of these hydrogel-based bioinks in the broader context of tissue engineering and regenerative medicine, offering valuable insights for future investigations.

Keywords
3D bioprinting
Hydrogel
Bioink
Tissue engineering
Bionic scaffold
References

Hosseini V, Maroufi N F, Saghati S, et al., 2019, Current progress in hepatic tissue regeneration by tissue engineering. J Transl Med, 17(1): 383. https://doi.org/10.1186/s12967-019-02137-6

Beheshtizadeh N, Lotfibakhshaiesh N, Pazhouhnia Z, et al., 2019, A review of 3D bio-printing for bone and skin tissue engineering: A commercial approach. J Mater Sci, 55(9): 3729–3749. https://doi.org/10.1007/s10853-019-04259-0

Ozbolat IT, Peng W, Ozbolat V, 2016, Application areas of 3D bioprinting. Drug Discov Today, 21(8): 1257–1271. https://doi.org/10.1016/j.drudis.2016.04.006

Magalhaes RS, Williams JK, Yoo KW, et al., 2020, A tissue-engineered uterus supports live births in rabbits. Nat Biotechnol, 38(11): 1280–1287. https://doi.org/10.1038/s41587-020-0547-7

Hellström M, Bandstein S, Brännström M, 2016, Uterine tissue engineering and the future of uterus transplantation. Ann Biomed Eng, 45(7): 1718–1730. https://doi.org/10.1007/s10439-016-1776-2

Campo H, Cervelló I, Simón C, 2016, Bioengineering the uterus: An overview of recent advances and future perspectives in reproductive medicine. Ann Biomed Eng, 45(7): 1710–1717. https://doi.org/10.1007/s10439-016-1783-3

 

Sridhar R, Lakshminarayanan R, Madhaiyan K, et al., 2015, Electrosprayed nanoparticles and electrospun nanofibers based on natural materials: Applications in tissue regeneration, drug delivery and pharmaceuticals. Chem Soc Rev, 44(3): 790–814. https://doi.org/10.1039/c4cs00226a

Chen W, Xu Y, Li Y, et al., 2020, 3D printing electrospinning fiber-reinforced decellularized extracellular matrix for cartilage regeneration. Chem Eng J, 382: 122986. https://doi.org/10.1016/j.cej.2019.122986

 

Lai Y, Li Y, Cao H, et al., 2019, Osteogenic magnesium incorporated into PLGA/TCP porous scaffold by 3D printing for repairing challenging bone defect. Biomaterials, 197: 207–219. https://doi.org/10.1016/j.biomaterials.2019.01.013

Golzar H, Mohammadrezaei D, Yadegari A, et al., 2020, Incorporation of functionalized reduced graphene oxide/ magnesium nanohybrid to enhance the osteoinductivity capability of 3D printed calcium phosphate-based scaffolds. Compos Part B Eng, 185: 107749. https://doi.org/10.1016/j.compositesb.2020.107749

Zhang J, Wu G, Qiu J, 2021, Interactions between cells and biomaterials in tissue engineering: A review. Sheng Wu Gong Cheng Xue Bao, 37(8): 2668–2677.

Hassan M, Dave K, Chandrawati R, et al., 2019, 3D printing of biopolymer nanocomposites for tissue engineering: Nanomaterials, processing and structure-function relation. Eur Poly J, 121: 109340. https://doi.org/10.1016/j.eurpolymj.2019.109340

Aljohani W, Ullah MW, Zhang X, et al., 2018, Bioprinting and its applications in tissue engineering and regenerative medicine. Int J Biol Macromol, 107(Pt A): 261–275. https://doi.org/10.1016/j.ijbiomac.2017.08.171

Groll J, Burdick JA, Cho D W, et al., 2018, A definition of bioinks and their distinction from biomaterial inks. Biofabrication, 11(1): 013001. https://doi.org/10.1088/1758-5090/aaec52

 

Hospodiuk M, Dey M, Sosnoski D, et al., 2017, The bioink: A comprehensive review on bioprintable materials. Biotechnol Adv, 35(2): 217–239. https://doi.org/10.1016/j.biotechadv.2016.12.006

Kabirian F, Mozafari M, 2020, Decellularized ECM-derived bioinks: Prospects for the future. Methods, 171: 108–118. https://doi.org/10.1016/j.ymeth.2019.04.019

Yue K, Trujillo-De Santiago G, Alvarez MM, et al., 2015, Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials, 73: 254–271. https://doi.org/10.1016/j.biomaterials.2015.08.045

Blaeser A, Duarte Campos DF, Puster U, et al., 2016, Controlling shear stress in 3D bioprinting is a key factor to balance printing resolution and stem cell integrity. Adv Healthc Mater, 5(3): 326–333. https://doi.org/10.1002/adhm.201500677

Gungor-Ozkerim PS, Inci I, Zhang Y , et al., 2018, Bioinks for 3D bioprinting: An overview. Biomater Sci, 6(5): 915–946. https://doi.org/10.1039/c7bm00765e

Griffanti G, Rezabeigi E, Li J, et al., 2019, Rapid biofabrication of printable dense collagen bioinks of tunable properties. Adv Funct Mater, 30(4): 1903874. https://doi.org/10.1002/adfm.201903874

Ahlfeld T, Cidonio G, Kilian D, et al., 2017, Development of a clay based bioink for 3D cell printing for skeletal application. Biofabrication, 9(3): 034103. https://doi.org/10.1088/1758-5090/aa7e96

Rastin H, Ormsby RT, Atkins GJ, et al., 2020, 3D bioprinting of methylcellulose/gelatin-methacryloyl (MC/GelMA) bioink with high shape integrity. ACS Appl Bio Mater, 3(3): 1815–1826. https://doi.org/10.1021/acsabm.0c00169

Williams D, Thayer P, Martinez H, et al., 2018, A perspective on the physical, mechanical and biological specifications of bioinks and the development of functional tissues in 3D bioprinting. Bioprinting, 9: 19–36. https://doi.org/10.1016/j.bprint.2018.02.003

Garreta E, Oria R, Tarantino C, et al., 2017, Tissue engineering by decellularization and 3D bioprinting. Mater Today, 20(4): 166–178. https://doi.org/10.1016/j.mattod.2016.12.005

Deo KA, Singh KA, Peak CW, et al., 2020, Bioprinting 101: Design, fabrication, and evaluation of cell-laden 3D bioprinted scaffolds. Tissue Eng Part A, 26(5–6): 318–338. https://doi.org/10.1089/ten.TEA.2019.0298

Malda J, Visser J, Melchels FP, et al., 2013, 25th anniversary article: Engineering hydrogels for biofabrication. Adv Mater, 25(36): 5011–5028. https://doi.org/10.1002/adma.201302042

Matai I, Kaur G, Seyedsalehi A, et al., 2020, Progress in 3D bioprinting technology for tissue/organ regenerative engineering. Biomaterials, 226: 119536.

https://doi.org/10.1016/j.biomaterials.2019.119536

Jessop ZM, Al-Sabah A, Gardiner MD, et al., 2017, 3D bioprinting for reconstructive surgery: Principles, applications and challenges. J Plast Reconstr Aesthet Surg, 70(9): 1155–1170. https://doi.org/10.1016/j.bjps.2017.06.001

Jentsch S, Nasehi R, Kuckelkorn C, et al., 2021, Multiscale 3D bioprinting by nozzle-free acoustic droplet ejection. Small Methods, 5(6): e2000971.

Adine C, Ng KK, Rungarunlert S, et al., 2018, Engineering innervated secretory epithelial organoids by magnetic three-dimensional bioprinting for stimulating epithelial growth in salivary glands. Biomaterials, 180: 52–66.

Jessop ZM, Al-Sabah A, Gao N, et al., 2019, Printability of pulp derived crystal, fibril and blend nanocellulose-alginate bioinks for extrusion 3D bioprinting. Biofabrication, 11(4): 045006. https://doi.org/10.1088/1758-5090/ab0631

Pan W, Wallin TJ, Odent J, et al., 2019, Optical stereolithography of antifouling zwitterionic hydrogels. J Mater Chem B, 7(17): 2855–2864. https://doi.org/10.1039/c9tb00278b

You S, Li J, Zhu W, et al., 2018, Nanoscale 3D printing of hydrogels for cellular tissue engineering. J Mater Chem B, 6(15): 2187–2197. https://doi.org/10.1039/C8TB00301G

Hong H, Seo YB, Kim DY, et al., 2020, Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering. Biomaterials, 232: 119679. https://doi.org/10.1016/j.biomaterials.2019.119679

Ying GL, Jiang N, Maharjan S, et al., 2018, Aqueous two-phase emulsion bioink-enabled 3D bioprinting of porous hydrogels. Adv Mater, 30(50): e1805460. https://doi.org/10.1002/adma.201805460

Heo DN, Castro NJ, Lee SJ, et al., 2017, Enhanced bone tissue regeneration using a 3D printed microstructure incorporated with a hybrid nano hydrogel. Nanoscale, 9(16): 5055–5062. https://doi.org/10.1039/c6nr09652b

Miri AK, Nieto D, Iglesias L, et al., 2018, Microfluidics-enabled multimaterial maskless stereolithographic bioprinting. Adv Mater, 30(27): e1800242.

Kelly BE, Bhattacharya I, Heidari H, et al., 2019, Volumetric additive manufacturing via tomographic reconstruction. Science, 363(6431): 1045–1079.

Morris VB, Nimbalkar S, Younesi M, et al., 2017, Mechanical properties, cytocompatibility and manufacturability of chitosan:PEGDA hybrid-gel scaffolds by stereolithography. Ann Biomed Eng, 45(1): 286–296. https://doi.org/10.1007/s10439-016-1643-1

Shen Y, Tang H, Huang X, et al., 2020, DLP printing photocurable chitosan to build bio-constructs for tissue engineering. Carbohydr Polym, 235: 115970. https://doi.org/10.1016/j.carbpol.2020.115970

Ouyang L, Armstrong JPK, Lin Y, et al., 2020, Expanding and optimizing 3D bioprinting capabilities using complementary network bioinks. Sci Adv, 6(38).

 

Kesti M, Muller M, Becher J, et al., 2015, A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation. Acta Biomater, 11: 162–172. https://doi.org/10.1016/j.actbio.2014.09.033

 

Kim W, Kim G, 2019, Collagen/bioceramic-based composite bioink to fabricate a porous 3D hASCs-laden structure for bone tissue regeneration. Biofabrication, 12(1): 015007. https://doi.org/10.1088/1758-5090/ab436d

Alexander FA, Jr., Johnson L, Williams K, et al., 2019, A parameter study for 3D-printing organized nanofibrous collagen scaffolds using direct-write electrospinning. Materials (Basel), 12(24): 4131. https://doi.org/10.3390/ma12244131

Axpe E, Oyen M, 2016, Applications of alginate-based bioinks in 3D bioprinting. Int J Mol Sci, 17(12): 1976. https://doi.org/10.3390/ijms17121976

Kyle S, Jessop ZM, Al-Sabah A, et al., 2017, ‘Printability’ of candidate biomaterials for extrusion based 3D printing: State-of-the-art. Adv Healthc Mater, 6(16). https://doi.org/10.1002/adhm.201700264

 

Cattelan G, Guerrero Gerbolés A, Foresti R, et al., 2020, Alginate formulations: Current developments in the race for hydrogel-based cardiac regeneration. Front Bioeng Biotechnol, 8: 00414. https://doi.org/10.3389/fbioe.2020.00414

Freeman FE, Kelly DJ, 2017, Tuning alginate bioink stiffness and composition for controlled growth factor delivery and to spatially direct MSC fate within bioprinted tissues. Sci Rep, 7(1): 17042. https://doi.org/10.1038/s41598-017-17286-1

Trachsel L, Johnbosco C, Lang T, et al., 2019, Double-network hydrogels including enzymatically crosslinked poly-(2-alkyl-2-oxazoline)s for 3D bioprinting of cartilage-engineering constructs. Biomacromolecules, 20(12): 4502–4511. https://doi.org/10.1021/acs.biomac.9b01266

Liu C, Qin W, Wang Y, et al., 2021, 3D printed gelatin/ sodium alginate hydrogel scaffolds doped with nano-attapulgite for bone tissue repair. Int J Nanomedicine, 16: 8417–8432.

Chen Q, Tian X, Fan J, et al., 2020, An interpenetrating alginate/gelatin network for three-dimensional (3D) cell cultures and organ bioprinting. Molecules, 25(3): 756. https://doi.org/10.3390/molecules25030756

Yang X, Lu Z, Wu H, et al., 2018, Collagen-alginate as bioink for three-dimensional (3D) cell printing based cartilage tissue engineering. Mater Sci Eng C Mater Biol Appl, 83: 195–201. https://doi.org/10.1016/j.msec.2017.09.002

Hong S, Sycks D, Chan HF, et al., 2015, 3D printing of highly stretchable and tough hydrogels into complex, cellularized structures. Adv Mater, 27(27): 4035–4040. https://doi.org/10.1002/adma.201501099

Rg A, Lts A, Ab A, et al., 2022, Development and evaluation of a multicomponent bioink consisting of alginate, gelatin, diethylaminoethyl cellulose and collagen peptide for 3D bioprinting of tissue construct for drug screening application. Int J Biol Macromol, 207: 278–288.

Gritsch L, Motta FL, Contessi Negrini N, et al., 2018, Crosslinked gelatin hydrogels as carriers for controlled heparin release. Mater Lett, 228: 375–378. https://doi.org/10.1016/j.matlet.2018.06.047

Contessi Negrini N, Celikkin N, Tarsini P, et al., 2020, Three-dimensional printing of chemically crosslinked gelatin hydrogels for adipose tissue engineering. Biofabrication, 12(2): 025001. https://doi.org/10.1088/1758-5090/ab56f9

Yang G, Xiao Z, Long H, et al., 2018, Assessment of the characteristics and biocompatibility of gelatin sponge scaffolds prepared by various crosslinking methods. Sci Rep, 8(1): 1616. https://doi.org/10.1038/s41598-018-20006-y

Contessi Negrini N, Tarsini P, Tanzi MC, et al., 2018, Chemically crosslinked gelatin hydrogels as scaffolding materials for adipose tissue engineering. J Appl Polym Sci, 136(8): 47104. https://doi.org/10.1002/app.47104

Chouhan D, Mandal BB, 2020, Silk biomaterials in wound healing and skin regeneration therapeutics: From bench to bedside. Acta Biomater, 103: 24–51. https://doi.org/10.1016/j.actbio.2019.11.050

Xiong S, Zhang X, Lu P, et al., 2017, A gelatin-sulfonated silk composite scaffold based on 3D printing technology enhances skin regeneration by stimulating epidermal growth and dermal neovascularization. Sci Rep, 7(1): 4288. https://doi.org/10.1038/s41598-017-04149-y

Costa JB, Park J, Jorgensen AM, et al., 2020, 3D bioprinted highly elastic hybrid constructs for advanced fibrocartilaginous tissue regeneration. Chem Mater, 32(19): 8733–8746. https://doi.org/10.1021/acs.chemmater.0c03556

Castilho MA-O, Levato RA-O, Bernal PN, et al., 2021, Hydrogel-based bioinks for cell electrowriting of well-organized living structures with micrometer-scale resolution. Biomacromolecules, 22(2): 855–866.

Pati F, Jang J, Ha DH, et al., 2014, Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink. Nat Commun, 5: 3935.

Choi YJ, Park HA-O, Ha DH, et al., 2021, 3D bioprinting of in vitro models using hydrogel-based bioinks. Polymers (Basel), 13(3): 366.

Zhuang T, Li X, Deng Q, et al., 2020, A GelMA/DECM/ nanoclay composite biomaterial ink for printing 3D scaffolds for primary hepatocytes cultivation. Mater Lett, 274: 128034. https://doi.org/10.1016/j.matlet.2020.128034

Khati V, Ramachandraiah H, Pati FA-O, et al., 2022, 3D bioprinting of multi-material decellularized liver matrix hydrogel at physiological temperatures. Biosensors (Basel),12(7): 521.

Veiga A, Silva IV, Duarte MA-O, et al., 2021, Current trends on protein driven bioinks for 3D printing. Pharmaceutics, 13(9): 1444.

Shim JH, Kim JY, Park M, et al., 2011, Development of a hybrid scaffold with synthetic biomaterials and hydrogel using solid freeform fabrication technology. Biofabrication, 3(3): 034102.

 

Casali DM, Yost MJ, Matthews MA, 2018, Eliminating glutaraldehyde from crosslinked collagen films using supercritical CO2. J Biomed Mater Res Part A, 106(1): 86–94. https://doi.org/10.1002/jbm.a.36209

 

Hwang Y-J, Larsen J, Krasieva TB, et al., 2011, Effect of genipin crosslinking on the optical spectral properties and structures of collagen hydrogels. ACS Appl Mater Interfaces, 3(7): 2579–2584. https://doi.org/10.1021/am200416h

Bax DV, Davidenko N, Gullberg D, et al., 2017, Fundamental insight into the effect of carbodiimide crosslinking on cellular recognition of collagen-based scaffolds. Acta Biomater, 49: 218–234. https://doi.org/10.1016/j.actbio.2016.11.059

Bax DV, Davidenko N, Hamaia SW, et al., 2019, Impact of UV- and carbodiimide-based crosslinking on the integrin-binding properties of collagen-based materials. Acta Biomater, 100: 280–291. https://doi.org/10.1016/j.actbio.2019.09.046

Serna JA-O, Florez SA-O, Talero VA, et al., 2019, Formulation and characterization of a SIS-based photocrosslinkable bioink. Polymers (Basel), 11(3): 569.

Madaghiele M, Calò E, Salvatore L, et al., 2016, Assessment of collagen crosslinking and denaturation for the design of regenerative scaffolds. J Biomed Mater Res Part A, 104(1): 186–194. https://doi.org/10.1002/jbm.a.35554

Clark CC, Yoo KM, Sivakumar H, et al., 2022, Immersion bioprinting of hyaluronan and collagen bioink-supported 3D patient-derived brain tumor organoids. Biomed Mater, 18(1).

Zhou Q, Yang K, He J, et al., 2019, A novel 3D-printable hydrogel with high mechanical strength and shape memory properties. J Mater Chem C, 7(47): 14913–14922. https://doi.org/10.1039/c9tc04945b

 

Chen H, Cheng R, Zhao X, et al., 2019, An injectable self-healing coordinative hydrogel with antibacterial and angiogenic properties for diabetic skin wound repair. NPG Asia Mater, 11(1). https://doi.org/10.1038/s41427-018-0103-9

Jia W, Gungor-Ozkerim PS, Zhang YS, et al., 2016, Direct 3D bioprinting of perfusable vascular constructs using a blend bioink. Biomaterials, 106: 66. https://doi.org/10.1016/j.biomaterials.2016.07.038

 

Kolesky DB, Truby RL, Gladman AS, et al., 2014, 3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs. Adv Mater, 26(19): 3124–3130. https://doi.org/10.1002/adma.201305506

Muller M, Becher J, Schnabelrauch M, et al., 2015, Nanostructured pluronic hydrogels as bioinks for 3D bioprinting. Biofabrication, 7(3): 035006. https://doi.org/10.1088/1758-5090/7/3/035006

Yu YH, Lee D, Hsu YH, et al., 2020, A three-dimensional printed polycaprolactone scaffold combined with co-axially electrospun vancomycin/ceftazidime/bone morphological protein-2 sheath-core nanofibers for the repair of segmental bone defects during the masquelet procedure. Int J Nanomed, 15: 913–925. https://doi.org/10.2147/ijn.S238478

Zhang YS, Davoudi F, Walch P, et al., 2016, Bioprinted thrombosis-on-a-chip. Lab Chip, 16(21): 4097–4105. https://doi.org/10.1039/c6lc00380j

Xu Y, Hu Y, Liu C, et al., 2018, A novel strategy for creating tissue-engineered biomimetic blood vessels using 3D bioprinting technology. Materials, 11(9): 1581. https://doi.org/10.3390/ma11091581

Karyappa RA-O, Goh WH, Hashimoto MA-O, 2022, Embedded core-shell 3D printing (eCS3DP) with low-viscosity polysiloxanes. ACS Appl Mater Interfaces, 14(36): 41520–41530.

 

Liu W, Heinrich MA, Zhou Y, et al., 2017, Extrusion bioprinting of shear-thinning gelatin methacryloyl bioinks. Adv Healthc Mater, 6(12). https://doi.org/10.1002/adhm.201601451

Zhao X, Lang Q, Yildirimer L, et al., 2016, Photocrosslinkable gelatin hydrogel for epidermal tissue engineering. Adv Healthc Mater, 5(1): 108–118. https://doi.org/10.1002/adhm.201500005

Bertassoni LE, Cardoso JC, Manoharan V, et al., 2014, Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels. Biofabrication, 6(2): 024105. https://doi.org/10.1088/1758-5082/6/2/024105

Zhang H, Cong Y, Osi AR, et al., 2020, Direct 3D printed biomimetic scaffolds based on hydrogel microparticles for cell spheroid growth. Adv Funct Mater, 30(13): 1910573. https://doi.org/10.1002/adfm.201910573

 

Sahranavard M, Zamanian A, Ghorbani F, et al., 2020, A critical review on three dimensional-printed chitosan hydrogels for development of tissue engineering. Bioprinting, 17: e00063. https://doi.org/10.1016/j.bprint.2019.e00063

Intini C, Elviri L, Cabral J, et al., 2018, 3D-printed chitosan-based scaffolds: An in vitro study of human skin cell growth and an in-vivo wound healing evaluation in experimental diabetes in rats. Carbohydr Polym, 199: 593–602. https://doi.org/10.1016/j.carbpol.2018.07.057

Chang HK, Yang DH, Ha MY, et al., 2022, 3D printing of cell-laden visible light curable glycol chitosan bioink for bone tissue engineering. Carbohydr Polym, 287: 1879–1344.

Kim SH, Yeon YK, Lee JM, et al., 2018, Precisely printable and biocompatible silk fibroin bioink for digital light processing 3D printing. Nat Commun, 9(1). https://doi.org/10.1038/s41467-018-03759-y

Carrow JK, Gaharwar AK, 2015, Bioinspired polymeric nanocomposites for regenerative medicine. Macromol Chem Phys, 216(3): 248–264. https://doi.org/10.1002/macp.201400427

Zhu K, Shin SR, Van Kempen T, et al., 2017, Gold nanocomposite bioink for printing 3D cardiac constructs. Adv Funct Mater, 27(12): 1605352. https://doi.org/10.1002/adfm.201605352

Navaei A, Saini H, Christenson W, et al., 2016, Gold nanorod-incorporated gelatin-based conductive hydrogels for engineering cardiac tissue constructs. Acta Biomater, 41: 133–146. https://doi.org/10.1016/j.actbio.2016.05.027

Gao L, Zhou Y, Peng J, et al., 2019, A novel dual-adhesive and bioactive hydrogel activated by bioglass for wound healing. NPG Asia Mater, 11(1). https://doi.org/10.1038/s41427-019-0168-0

Gonçalves EM, Oliveira FJ, Silva RF, et al., 2016, Three-dimensional printed PCL-hydroxyapatite scaffolds filled with CNTs for bone cell growth stimulation. J Biomed Mater Res Part B Appl Biomater, 104(6): 1210–1219. https://doi.org/10.1002/jbm.b.33432

Zheng X, Zhang X, Wang Y, et al., 2021, Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration. Bioact Mater, 6(10): 3485–3495.

Ding Y, Liu X, Zhang J, et al., 2022, 3D printing polylactic acid polymer-bioactive glass loaded with bone cement for bone defect in weight-bearing area. Front Bioeng Biotechnol, 10: 947521.

 

Gao F, Xu Z, Liang Q, et al., 2019, Osteochondral regeneration with 3D-printed biodegradable high-strength supramolecular polymer reinforced-gelatin hydrogel scaffolds. Adv Sci (Weinh), 6(15): 1900867. https://doi.org/10.1002/advs.201900867

Aihemaiti P, Jiang H, Aiyiti W, et al., Optimization of 3D printing parameters of biodegradable polylactic acid/ hydroxyapatite composite bone plates. Int J Bioprint, 8(1): 490.

Ergul NM, Unal S, Kartal I, et al., 2019, 3D printing of chitosan/ poly(vinyl alcohol) hydrogel containing synthesized hydroxyapatite scaffolds for hard-tissue engineering. Polymer Testing, 79: 106006. https://doi.org/10.1016/j.polymertesting.2019.106006

Tomás H, Alves CS, Rodrigues J, 2018, Laponite®: A key nanoplatform for biomedical applications? Nanomedicine, 14(7): 2407–2420. https://doi.org/10.1016/j.nano.2017.04.016

 

Afewerki S, Magalhaes L, Silva A D R, et al., 2019, Bioprinting a synthetic smectic clay for orthopedic applications. Adv Healthc Mater, 8(13): e1900158. https://doi.org/10.1002/adhm.201900158

Cheng Z, Landish B, Chi Z, et al., 2018, 3D printing hydrogel with graphene oxide is functional in cartilage protection by influencing the signal pathway of Rank/Rankl/OPG. Mater Sci Eng C Mater Biol Appl, 82: 244–252.

Li L, Qin S, Peng J, et al., 2020, Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs. Int J Biol Macromol, 145: 262–271.

Dasari Shareena TP, Mcshan D, Dasmahapatra AK, et al., 2018, A review on graphene-based nanomaterials in biomedical applications and risks in environment and health. Nanomicro Lett, 10(3): 53. https://doi.org/10.1007/s40820-018-0206-4

Shin SR, Li YC, Jang HL, et al., 2016, Graphene-based materials for tissue engineering. Adv Drug Deliv Rev, 105(Pt B): 255–274. https://doi.org/10.1016/j.addr.2016.03.007

Syama S, Mohanan PV, 2019, Comprehensive application of graphene: Emphasis on biomedical concerns. Nano-Micro Lett, 11(1): 6. https://doi.org/10.1007/s40820-019-0237-5

Nie C, Ma L, Li S, et al., 2019, Recent progresses in graphene based bio-functional nanostructures for advanced biological and cellular interfaces. Nano Today, 26: 57–97. https://doi.org/10.1016/j.nantod.2019.03.003

Jo H, Sim M, Kim S, et al., 2017, Electrically conductive graphene/polyacrylamide hydrogels produced by mild chemical reduction for enhanced myoblast growth and differentiation. Acta Biomater, 48: 100–109. https://doi.org/10.1016/j.actbio.2016.10.035

Park J, Choi JH, Kim S, et al., 2019, Micropatterned conductive hydrogels as multifunctional muscle-mimicking biomaterials: Graphene-incorporated hydrogels directly patterned with femtosecond laser ablation. Acta Biomater, 97: 141–153. https://doi.org/10.1016/j.actbio.2019.07.044

Hu Y, Han W, Huang G, et al., 2016, Highly stretchable, mechanically strong, tough, and self-recoverable nanocomposite hydrogels by introducing strong ionic coordination interactions. Macromol Chem Phys, 217(24): 2717–2725. https://doi.org/10.1002/macp.201600398

Li J, Liu X, Crook J M, et al., 2022, Development of 3D printable graphene oxide based bio-ink for cell support and tissue engineering. Front Bioeng Biotechnol, 10: 994776.

Cheng Z, Landish B, Chi Z, et al., 2018, 3D printing hydrogel with graphene oxide is functional in cartilage protection by influencing the signal pathway of Rank/Rankl/OPG. Mater Sci Eng C Mater Biol Appl, 82: 244–252. https://doi.org/10.1016/j.msec.2017.08.069

Choe G, Oh S, Seok JM, et al., 2019, Graphene oxide/ alginate composites as novel bioinks for three-dimensional mesenchymal stem cell printing and bone regeneration applications. Nanoscale, 11(48): 23275–23285. https://doi.org/10.1039/c9nr07643c

Huang CT, Kumar Shrestha L, Ariga K, et al., 2017, A graphene-polyurethane composite hydrogel as a potential bioink for 3D bioprinting and differentiation of neural stem cells. J Mater Chem B, 5(44): 8854–8864. https://doi.org/10.1039/c7tb01594a

Lee SJ, Zhu W, Nowicki M, et al., 2018, 3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration. J Neural Eng, 15(1): 016018. https://doi.org/10.1088/1741-2552/aa95a5

Sanjuan-Alberte P, Whitehead C, Jones J N, et al., 2022, Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs. iScience, 25(7): 104552.

Li L, Qin S, Peng J, et al., 2020, Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs. Int J Biol Macromol, 145: 262–271. https://doi.org/10.1016/j.ijbiomac.2019.12.174

Ho CM, Mishra A, Lin P T, et al., 2017, 3D printed polycaprolactone carbon nanotube composite scaffolds for cardiac tissue engineering. Macromol Biosci, 17(4). https://doi.org/10.1002/mabi.201600250

Ergul NM, Unal S, Kartal I, et al., 2019, 3D printing of chitosan/ poly(vinyl alcohol) hydrogel containing synthesized hydroxyapatite scaffolds for hard-tissue engineering. Polym Test, 79: 106006. https://doi.org/10.1016/j.polymertesting.2019.106006

Cheng Z, Landish B, Chi Z, et al., 2018, 3D printing hydrogel with graphene oxide is functional in cartilage protection by influencing the signal pathway of Rank/Rankl/OPG. Mater Sci Eng C, 82: 244–252. https://doi.org/10.1016/j.msec.2017.08.069

Navaei A, Saini H, Christenson W, et al., 2016, Gold nanorod-incorporated gelatin-based conductive hydrogels for engineering cardiac tissue constructs. Acta Biomater, 41: 133–146. https://doi.org/10.1016/j.actbio.2016.05.027

Khalili Fard J, Jafari S, Eghbal MA, 2015, A review of molecular mechanisms involved in toxicity of nanoparticles. Adv Pharm Bull, 5(4): 447–454.

Wan B, Wang ZX, Lv QY, et al., 2013, Single-walled carbon nanotubes and graphene oxides induce autophagosome accumulation and lysosome impairment in primarily cultured murine peritoneal macrophages. Toxicol Lett, 221(2): 118–127.

Yuan X, Zhang X, Sun L, et al., 2019, Cellular toxicity and immunological effects of carbon-based nanomaterials. Part Fibre Toxicol, 16: 1743–8977.

Mohammadinejad R, Kumar A, Ranjbar-Mohammadi M, et al., 2020, Recent advances in natural gum-based biomaterials for tissue engineering and regenerative medicine: A review. Polymers (Basel), 12(1): 176. https://doi.org/10.3390/polym12010176

Chen W, Xu Y, Liu Y, et al., 2019, Three-dimensional printed electrospun fiber-based scaffold for cartilage regeneration. Mater Des, 179: 107886. https://doi.org/10.1016/j.matdes.2019.107886

Dufresne A, 2013, Nanocellulose: A new ageless bionanomaterial. Mater Today, 16(6): 220–227. https://doi.org/10.1016/j.mattod.2013.06.004

Piras CC, Fernández-Prieto S, De Borggraeve WM, 2017, Nanocellulosic materials as bioinks for 3D bioprinting. Biomater Sci, 5(10): 1988–1992. https://doi.org/10.1039/c7bm00510e

Leppiniemi J, Lahtinen P, Paajanen A, et al., 2017, 3D printable bioactivated nanocellulose-alginate hydrogels. ACS Appl Mater Interfaces, 9(26): 21959–21970.

Sultan S, Mathew AP, 2018, 3D printed scaffolds with gradient porosity based on a cellulose nanocrystal hydrogel. Nanoscale, 10(9): 4421–4431. https://doi.org/10.1039/c7nr08966j

Alcala-Orozco CR, Mutreja I, Cui X, et al., 2020, Design and characterisation of multi-functional strontium-gelatin nanocomposite bioinks with improved print fidelity and osteogenic capacity. Bioprinting, 18: e00073. https://doi.org/10.1016/j.bprint.2019.e00073

Annabi N, Shin SR, Tamayol A, et al., 2016, Highly elastic and conductive human-based protein hybrid hydrogels. Adv Mater, 28(1): 40–49: 40–49. https://doi.org/10.1002/adma.201503255

Hribar KC, Meggs K, Liu J, et al., 2015, Three-dimensional direct cell patterning in collagen hydrogels with near-infrared femtosecond laser. Sci Rep, 5: 17203. https://doi.org/10.1038/srep17203

Vijayavenkataraman S, Lu WF, Fuh JY, 2016, 3D bioprinting of skin: A state-of-the-art review on modelling, materials, and processes. Biofabrication, 8(3): 032001. https://doi.org/10.1088/1758-5090/8/3/032001

Sheikholeslam M, Wright MEE, Jeschke MG, et al., 2018, Biomaterials for skin substitutes. Adv Healthc Mater, 7(5). https://doi.org/10.1002/adhm.201700897

Ng WL, Wang S, Yeong WY, et al., 2016, Skin bioprinting: Impending reality or fantasy? Trends Biotechnol, 34(9): 689–699. https://doi.org/10.1016/j.tibtech.2016.04.006

Shi Y, Xing TL, Zhang HB, et al., 2018, Tyrosinase-doped bioink for 3D bioprinting of living skin constructs. Biomed Mater, 13(3): 035008. https://doi.org/10.1088/1748-605X/aaa5b6

Zhou F, Hong Y, Liang R, et al., 2020, Rapid printing of bio-inspired 3D tissue constructs for skin regeneration. Biomaterials, 258: 120287. https://doi.org/10.1016/j.biomaterials.2020.120287

Ozbolat IT, 2015, Bioprinting scale-up tissue and organ constructs for transplantation. Trends Biotechnol, 33(7): 395–400. https://doi.org/10.1016/j.tibtech.2015.04.005

Urciuolo A, Poli I, Brandolino L, et al., 2020, Intravital three-dimensional bioprinting. Nat Biomed Eng, 4(9): 901–915. https://doi.org/10.1038/s41551-020-0568-z

Albanna M, Binder KW, Murphy SV, et al., 2019, In situ bioprinting of autologous skin cells accelerates wound healing of extensive excisional full-thickness wounds. Sci Rep, 9(1): 1856. https://doi.org/10.1038/s41598-018-38366-w

Homan KA, Kolesky DB, Skylar-Scott MA, et al., 2016, Bioprinting of 3D convoluted renal proximal tubules on perfusable chips. Sci Rep, 6(1). https://doi.org/10.1038/srep34845

Zhang K, Fu Q, Yoo J, et al., 2017, 3D bioprinting of urethra with PCL/PLCL blend and dual autologous cells in fibrin hydrogel: An in vitro evaluation of biomimetic mechanical property and cell growth environment. Acta Biomater, 50: 154–164. https://doi.org/10.1016/j.actbio.2016.12.008

Ke D, Yi H, Est-Witte S, et al., 2019, Bioprinted trachea constructs with patient-matched design, mechanical and biological properties. Biofabrication, 12(1): 015022. https://doi.org/10.1088/1758-5090/ab5354

 

Ha D-H, Chae S, Lee JY, et al., 2021, Therapeutic effect of decellularized extracellular matrix-based hydrogel for radiation esophagitis by 3D printed esophageal stent. Biomaterials, 266: 120477. https://doi.org/10.1016/j.biomaterials.2020.120477

Zhu W, Qu X, Zhu J, et al., 2017, Direct 3D bioprinting of prevascularized tissue constructs with complex microarchitecture. Biomaterials, 124: 106–115. https://doi.org/10.1016/j.biomaterials.2017.01.042

Góra A, Pliszka D, Mukherjee S, et al., 2016, Tubular tissues and organs of human body—challenges in regenerative medicine. J Nanosci Nanotechnol, 16(1): 19–39. https://doi.org/10.1166/jnn.2016.11604

Virk JS, Zhang H, Nouraei R, et al., 2017, Prosthetic reconstruction of the trachea: A historical perspective. World J Clin Cases, 5(4): 128–133. https://doi.org/10.12998/wjcc.v5.i4.128

Lei D, Luo B, Guo Y, et al., 2019, 4-Axis printing microfibrous tubular scaffold and tracheal cartilage application. Sci China Mater, 62(12): 1910–1920. https://doi.org/10.1007/s40843-019-9498-5

Huo Y, Xu Y, Wu X, et al., 2022, Functional trachea reconstruction using 3D-bioprinted native-like tissue architecture based on designable tissue-specific bioinks. Adv Sci (Weinh), 9(29): e2202181.

Benjamin EJ, Blaha MJ, Chiuve SE, et al., 2017, Heart disease and stroke statistics—2017 update: A report from the American Heart Association. Circulation, 135(10): https://doi.org/10.1161/cir.0000000000000485

Noor N, Shapira A, Edri R, et al., 2019, 3D printing of personalized thick and perfusable cardiac patches and hearts. Adv Sci, 6(11): 1900344. https://doi.org/10.1002/advs.201900344

Shapira A, Noor N, Asulin M, et al., 2018, Stabilization strategies in extrusion-based 3D bioprinting for tissue engineering. Appl Phys Rev, 5(4): 041112. https://doi.org/10.1063/1.5055659

Adams F, Qiu T, Mark A, et al., 2017, Soft 3D-printed phantom of the human kidney with collecting system. Ann Biomed Eng, 45(4): 963–972. https://doi.org/10.1007/s10439-016-1757-5

Albanna M, Binder KW, Murphy SA-O, et al., 2019, In situ bioprinting of autologous skin cells accelerates wound healing of extensive excisional full-thickness wounds. Sci Rep, 9(1): 1856.

Noor N, Shapira A, Edri R, et al., 2019, 3D printing of personalized thick and perfusable cardiac patches and hearts. Adv Sci (Weinh), 6(11): 1900344.

Adams F, Qiu T, Mark A, et al., 2017, Soft 3D-printed phantom of the human kidney with collecting system. Ann Biomed Eng, 45(4): 963–972.

He Y, Yang F, Zhao H, et al., 2016, Research on the printability of hydrogels in 3D bioprinting. Sci Rep, 6(1): 29977. https://doi.org/10.1038/srep29977

Datta P, Ayan B, Ozbolat IT, 2017, Bioprinting for vascular and vascularized tissue biofabrication. Acta Biomater, 51: 1–20. https://doi.org/10.1016/j.actbio.2017.01.035

Ouyang L, Yao R, Zhao Y, et al., 2016, Effect of bioink properties on printability and cell viability for 3D bioplotting of embryonic stem cells. Biofabrication, 8(3): 035020. https://doi.org/10.1088/1758-5090/8/3/035020

Lee JM, Yeong WY, 2016, Design and printing strategies in 3D bioprinting of cell-hydrogels: A review. Adv Healthc Mater, 5(22): 2856–2865. https://doi.org/10.1002/adhm.201600435

Holzl K, Lin S, Tytgat L, et al., 2016, Bioink properties before, during and after 3D bioprinting. Biofabrication, 8(3): 032002. https://doi.org/10.1088/1758-5090/8/3/032002

 

Murphy SV, Atala A, 2014, 3D bioprinting of tissues and organs. Nat Biotechnol, 32(8): 773–785. https://doi.org/10.1038/nbt.2958

Chimene D, Kaunas R, Gaharwar AK, 2020, Hydrogel bioink reinforcement for additive manufacturing: A focused review of emerging strategies. Adv Mater, 32(1): e1902026. https://doi.org/10.1002/adma.201902026

Rana D, Ramasamy K, Leena M, et al., 2016, Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine. Biotechnol Prog, 32(3): 554–567. https://doi.org/10.1002/btpr.2262

Share
Back to top
International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing