AccScience Publishing / IJB / Volume 10 / Issue 6 / DOI: 10.36922/ijb.4692
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RESEARCH ARTICLE

Evaluation of 3D-bioprinted skin scaffolds in mice along with gold nanoparticle exposure

Yi Wang1,2 ,  Xin Ma1,3 ,  Xu Wu4 ,  Shuaideng Wang1 ,  Peng Peng1,2 ,  Guozhang Tang1,3 ,  Xinya Qin5,6 ,  Xinmeng Wang7* ,  Chenwei Wang1* ,  Jiangning Zhou5
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1 School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, China
2 First School of Clinical Medicine, Anhui Medical University, Hefei, Anhui, China
3 Second School of Clinical Medicine, Anhui Medical University, Hefei, Anhui, China
4 Chinese Academy of Sciences Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China
5 Institute of Brain Science, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China
6 Institute of Artificial Intelligence, Hefei Comprehensive National Science Center, Hefei, Anhui, China
7 Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China
IJB 2024, 10(6), 4692 https://doi.org/10.36922/ijb.4692
Submitted: 29 August 2024 | Accepted: 2 October 2024 | Published: 2 October 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

The progress in nanomedicine has sparked increasing concerns regarding its applications with biocompatible materials. Here, we assessed and optimized a three-dimensional (3D) bioprinting technique by testing various printing parameters with multiple cell types. Cell-laden scaffolds were designed, cultivated, imaged, and transplanted onto the dorsal skin of nude mice. The structure of bioprinted scaffolds retained its shape and dimensions with no cell migration between layers. Moreover, gold nanoparticles (GNPs) were intravenously administered to transplanted nude mice and aggregately deposited in the cell-laden scaffolds. Importantly, GNPs exhibited extensive accumulation in bioprinted scaffolds compared to natural skin and other organs in vivo. GNPs accumulated in the dermis of the transplanted scaffolds, while they stayed in the subcutaneous tissue of the natural skin with no permeation to the dermis, indicating a high absorption tendency of GNPs for artificial scaffolds. The results revealed a lack of similarity between the artificial skin scaffolds and natural skin, which may diminish their potential as artificial skin substitutes. Furthermore, the absorption property of 3D-bioprinted scaffolds suggests their potential as (i) a therapeutic method to absorb and excrete GNPs; and (ii) a strategy for targeted drug delivery of GNPs.  

Graphical abstract
Keywords
3D bioprinting
Skin scaffolds
Gold nanoparticles
Biodistribution
In vivo transplant
Funding
This work was supported by the STI2030-Major Projects (2022ZD0205202), the Anhui Province University Scientific Research Project (KJ2021A0212, 2022AH051169), the Anhui Medical University Research Funding (2021xkj008), the University Synergy Innovation Program of Anhui Province (GXXT-2022-030), and the College Students’ Innovation and Entrepreneurship Training Program of Anhui Province (S202210366019).
Conflict of interest
The authors declare no conflict of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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