AccScience Publishing / IJB / Volume 10 / Issue 3 / DOI: 10.36922/ijb.1673
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RESEARCH ARTICLE

Increased stiffness of extracellular matrix enhanced chemoresistance in 3D-bioprinted ovarian cancer model

Ying Shan1† ,  Mingchang Pang2† ,  Liqian Wang3† ,  Yixin Mao4† ,  Ruiyi Yan2 ,  Chang Zhou5 ,  Jingyuan Ji5 ,  Yilei Mao2 ,  Ying Jin1* ,  Huayu Yang2*
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1 Department of Obstetrics and Gynecology, National Clinical Research Center for Obstetric & Gynecologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
2 Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
3 Department of Gynecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China
4 Department of Geriatrics, Shanghai Eighth Peoples Hospital, Shanghai, China
5 Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing, China
IJB 2024, 10(3), 1673 https://doi.org/10.36922/ijb.1673
Submitted: 24 August 2023 | Accepted: 21 November 2023 | Published: 18 January 2024
(This article belongs to the Special Issue Bioprinting process for tumor model development)
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Ovarian cancer is a gynecological malignancy with a high mortality rate. The ovarian cancer microenvironment is a crucial factor affecting the overall and progression-free survival rates of patients with ovarian cancer. The biophysical factors of the tumor microenvironment, such as stiffness, can affect the gene expression and behavior of tumor cells. In this study, we utilized 3D bioprinting technology to construct ovarian cancer tumor models with varying levels of stiffness in vitro to investigate the effect of extracellular matrix stiffness on drug resistance of tumor cells. Our findings indicate that increasing the stiffness of extracellular matrix can attenuate the sensitivity of tumor cells to chemotherapeutic agents. Additionally, the increased stiffness of 3D tumor model may promote malignant phenotypes, such as tumor stemness and tumor progression.

Keywords
3D bioprinting technology
Ovarian cancer
Tumor microenvironment
Stiffness
Drug resistance
Funding
This work was supported by the National Natural Science Foundation of China (Grant No. 32271470), National High Level Hospital Clinical Research Funding (2022-PUMCH-C-045), Beijing Natural Science Foundation (7212077), and CAMS Innovation Fund for Medical Sciences (CIFMS) (No.2021-I2M-1-058).
Conflict of interest
The authors declare that the research was carried out without any commercial or financial relationships that could be perceived as a potential conflict of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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