AccScience Publishing / IJB / Volume 0 / Issue 0 / DOI: 10.36922/ijb.1152

Low-temperature deposition 3D printing biotin-doped PLGA/β-TCP scaffold for repair of bone defects in osteonecrosis of femoral head

Peng Xue1,2 Xiaoxue Tan3 Hongzhong Xi1,2 Hao Chen1,2 Shuai He1,2 Guangquan Sun1,2 Changyuan Gu1,2 Xiaohong Jiang3 Bin Du1,2* Xin Liu1,2*
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1 Department of Orthopedics, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China
2 Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China
3 International Chinese-Belorussian Scientific Laboratory on Vacuum-Plasma Technology, Nanjing University of Science and Technology, Nanjing 210094, China
Submitted: 26 June 2023 | Accepted: 10 August 2023 | Published: 12 September 2023
(This article belongs to the Special Issue Biomedical application of 3D Bioprinting)
© 2023 by the Author(s). Licensee AccScience Publishing, Singapore. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( )

The removal of necrotic bone and implantation of bone repair materials is an effective treatment for osteonecrosis of the femoral head (ONFH)-type bone defects, but there are currently no clinically applicable bone repair materials. In this study, a biotin-doped bone repair scaffold was created using low-temperature deposition (LTD) three-dimensional (3D) printing technology, and its ability to repair bone defects in ONFH was evaluated. The scaffold was characterized in vitro, and its cytotoxicity and osteogenic capacity were assessed by co-culturing the scaffold with rat bone marrow mesenchymal stem cells. The scaffolds were implanted in an animal model of ONFH-type bone defects, and the effect of scaffolds on promoting bone repair was evaluated by means of radiology and histopathology. LTD 3D-printed biotin-doped scaffolds showed cancellous bone-like structures without inducing cytotoxicity, whereas high-biotin β-TCP scaffolds (HBPT; containing 2% biotin) promoted osteogenic differentiation more effectively. Experiments on animals revealed that the effect of HBPT on bone repair was significantly superior to that of other groups. The in vivo biocompatibility of HBPT was confirmed by blood analysis and hematoxylin and eosin staining of the main organs. In conclusion, biotin-doped scaffolds can be used to treat ONFH-type bone defects by virtue of their ability in promoting bone regeneration.

Low-temperature deposition
Bone repair
Osteonecrosis of the femoral head
Bone tissue engineering scaffold
This research was funded by National Natural Science Foundation of China (No. 82074471), Jiangsu Provincial Commission of Health and Family Planning (No. K2019027), Priority Academic Program Development of Jiangsu Higher Education Institutions (No. 035062005001), and Jiangsu Graduate Practice and Innovation Plan (No. SJCX22_0769).
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Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing