AccScience Publishing / MI / Volume 1 / Issue 2 / DOI: 10.36922/mi.3896
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ORIGINAL RESEARCH ARTICLE

Hydrogen alleviates non-alcoholic fatty liver disease in mice by regulating intestinal flora

Yu Wang1,2† ,  Fan Zhang1† ,  Yan Tian2 ,  Yunxi Chen2 ,  Jianjun Zhou2* ,  Youzhen Wei2,3,4,5*
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1 Department of Microbiology and Immunology, Shanxi Medical University, Taiyuan, Shanxi, China
2 Research Center for Translational Medicine, Tongji University Affiliated East Hospital, Shanghai, China
3 Hydrogen Medicine Center, the Affiliated Hospital of Qingdao University Taian City Central Hospital, Taian, Shandong, China
4 Research Center for Translational Medicine, Jinan People’s Hospital, Shandong First Medical University, Jinan, Shandong, China
5 Department of Rehabilitation Medicine, Jinan Hospital (Jinan City Rehabilitation Hospital), Jinan, Shandong, China
MI 2024, 1(2), 70–80; https://doi.org/10.36922/mi.3896
Submitted: 8 June 2024 | Accepted: 29 August 2024 | Published: 29 October 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Despite being the most common form of chronic liver disease, there are still no approved drugs for thetreatment of non-alcoholic fatty liver disease (NAFLD). The aim of this study was to elucidate the therapeutic effects and possible mechanisms of hydrogen (H2) inhalation in mice with NAFLD. Male C57BL/6 mice (6 weeks old) were fed either a 60% fat diet (high-fat diet [HFD]) or a 10% fat diet (normal diet) for 11 weeks. Then, H2 was administered to random HFD-fed mice for another 11 weeks before they were euthanized. Biochemical analysis of serum samples, histological analysis of liver and ileum samples, 16S rRNA sequencing analysis of stool samples, and analysis of the expression levels of related factors by enzyme-linked immunosorbent assay were conducted to determine the effect of H2 intervention on NAFLD. H2 inhalation alleviated hyperglycemia and impaired glucose tolerance; decreased the serum concentrations of triglycerides, cholesterol, alanine aminotransferase, aspartate aminotransferase, lipopolysaccharide, and tumor necrosis factor-alpha; and ameliorated liver injury by a HFD, although no weight loss was observed. Interestingly, H2 inhalation increased the relative abundance of Akkermansia muciniphila and decreased the Firmicutes-to-Bacteroidia ratio in the intestinal tract of NAFLD mice. These data indicate that H2 alleviated the symptoms of NAFLD by increasing the abundance of A. muciniphila in the intestine. Thus, H2 may be a new potential treatment strategy for patients with NAFLD.

Keywords
Hydrogen
Non-alcoholic fatty liver disease
Intestinal flora
High-fat diet
Funding
This study was supported by the Small and Micro Science and Technology Project grant (No. 20004) of the National Health Commission, and the Shanxi Provincial Natural Fund Project (202203021211239).
Conflict of interest
The authors declare they have no competing interests.
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Microbes & Immunity, Electronic ISSN: 3029-2883 Print ISSN: 3041-0886, Published by AccScience Publishing
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