AccScience Publishing / JCBP / Online First / DOI: 10.36922/JCBP025260046
BRIEF REPORT

Hourly assessments of single-item immune fitness demonstrate its consistency during the day

Emina Išerić1 Guusje A. Ulijn1 Pauline A. Hendriksen1 Aurora J. A. E. van de Loo1 Johan Garssen1,2 Joris C. Verster1,3,4*
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1 Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands
2 Danone Global Research and Innovation Center, Utrecht, the Netherlands
3 Cognitive Neurophysiology, Department of Child and Adolescent Psychiatry, Faculty of Medicine, Dresden University of Technology, Dresden, Saxony, Germany
4 Centre for Mental Health and Brain Sciences, Swinburne University, Melbourne, Victoria, Australia
Received: 23 June 2025 | Revised: 28 August 2025 | Accepted: 16 September 2025 | Published online: 10 October 2025
© 2025 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Immune fitness is the body’s capacity to respond to health challenges (such as infections) by activating an appropriate immune response. Adequate immune fitness is crucial to maintaining health and preventing and resolving disease. Control day data from an experimental study among 29 healthy volunteers were evaluated to determine the consistency of immune fitness ratings throughout the day. The single-item immune fitness scale was completed hourly, from 09:30 to 15:30. As a comparison, mood was assessed with the Profiles of Mood States-Short Form, including subscales for vigor, fatigue, tension, depression, and anger. The analyses revealed that single-item immune fitness scores were stable throughout the day. In contrast, as expected, assessments of fatigue and vigor varied throughout the day, showing more energy in the morning assessments and less energy in the afternoon assessments. Tension, depression, and anger scores also remained stable throughout the day. In conclusion, this study demonstrates that single-item immune fitness ratings remain consistent and stable throughout the day. Therefore, the time of day at which participants assess their immune fitness does not influence the outcomes in clinical trials. Future studies in larger, more diverse samples can be conducted to confirm these findings.

Keywords
Immune fitness
Immunity
Self-report
Mood; Anxiety
Depression
Tension
Anger
Vigor
Funding
None.
Conflict of interest
Over the past 36 months, Joris C. Verster has received research grants from Danone and Inbiose and has acted as a consultant/expert advisor to Eisai, KNMP, Med Solutions, Mozand, Red Bull, Sen-Jam Pharmaceutical, and Toast!. Joris C. Verster and Emina Išerić have received travel support from Sen-Jam Pharmaceutical. Joris C. Verster owns stock in Sen-Jam Pharmaceutical. Johan Garssen is a part-time employee of Nutricia Research and received research grants from Nutricia Research Foundation, Top Institute Pharma, Top Institute Food and Nutrition, GSK, STW, NWO, Friesland Campina, CCC, Raak-Pro, and the European Union. The other authors declare no conflicts of interest.
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Journal of Clinical and Basic Psychosomatics, Electronic ISSN: 2972-4414 Print ISSN: 3060-8562, Published by AccScience Publishing