Basic life values are known to be stable over a lifetime and are predictive of a wide range of attitudes and behaviors. However, they may fluctuate in response to environmental changes or challenges. This study, conducted in two large representative cohorts, aimed to investigate whether basic life values across different age groups remained consistent before and after the COVID-19 pandemic. Two large representative cohorts from the German general population were surveyed about their basic life values using the Short Schwartz Values Scale in 2018 (N = 2412) and 2022 (N = 2499). A comparison was made across age groups and time points (pre- and post-pandemic) using multivariate analysis of variance (MANOVA). Our findings revealed a slight discrepancy in values post-global health crisis compared to pre-crisis levels, with lower scores observed for achievement and benevolence and higher scores for hedonism and self-direction following the crisis. However, benevolence, universalism, stability, and self-direction maintained high mean scores across both time points. Younger individuals were more oriented toward hedonism and self-direction than older individuals. Differences in values between age groups were evident (partial eta²: 0.004–0.110), whereas differences before and after the pandemic were minor (partial eta² <0.004). Basic life values, as observed on a societal level, did not change significantly despite a short-term external stressor such as a pandemic. In conclusion, patients’ individual life values should be considered in psychosomatic counseling and treatment, as these values impact health behavior and may lead to mental health problems, such as embitterment, when compromised.