This Special Issue aims at highlighting the latest advances in the field of 3D bioprinting across research groups in Australia and beyond. Despite being geographically distant from the rest of the world, Australia has been at the centre of 3D bioprinting thanks to the extensive research in hydrogel development, novel vascularization strategies and other approaches with a particular focus on translation of findings from the bench to the bedside. This is possible thanks to the multidisciplinary teams across Australian research groups which bring experts from several key areas, including biomaterials, bioengineering and in-vitro pathophysiology modelling. This collection will serve as an example of the variety of research in 3D bioprinting that is presented at the Sixth Australian Bioprinting Workshop (AusBioprint^TM) in Sydney and welcomes submissions outside what presented during the event.