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In situ bioprinting: Tailored printing strategies for regenerative medicine

Chengwei Hu1,2 ,  Chenmin Wang1 ,  Shaoquan Bian1 ,  Weichen Qi3,4 ,  Bo Liu1 ,  Liangliang Wang1 ,  Chunyi Wen5 ,  Jun Wu3,6* ,  William W. Lu1,3 ,  Xiaoli Zhao1,2*
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1 Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy Sciences, Shenzhen, Guangdong, China
2 University of Chinese Academy of Sciences, Beijing, China
3 Department of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Hong Kong, China
4 Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
5 Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China
6 Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, Guangdong Engineering Technology Research Center for Orthopaedic Trauma Repair, Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, China
IJB 2024, 10(5), 3366 https://doi.org/10.36922/ijb.3366
Submitted: 4 April 2024 | Accepted: 17 May 2024 | Published: 24 July 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

In recent years, three-dimensional (3D) bioprinting has emerged as a revolutionary biological manufacturing technology. Despite significant progress, current bioprinting technologies face critical barriers, such as the need for in vitro maturation of printed tissues before implantation and challenges of prefabricated structures not matching the defect shapes. In situ bioprinting has been introduced to address these challenges by printing customized structures to the wound shape via direct deposition of biological inks at the tissue interface. This paper reviews strategies to optimize printing performance for enhanced tissue repair and analyzes the advantages, challenges, and future directions of in situ bioprinting technologies.

Keywords
Bioprinting
In situ bioprinting
Tissue regeneration
Bioinks
Handheld bioprinter
Funding
This work was supported by the National Key Research and Development Program of China (2018YFA0703100), Guangdong Basic and Applied Basic Research Foundation (2021A1515110902, 2021A1515110794), Shenzhen Science and Technology Funding (JCYJ20220530142206014), and the Shanghai Municipal Health Commission Health Industry Clinical Research Program for Youth (20224Y0184).
Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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