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RESEARCH ARTICLE

Treatment effects of 3D-printed PCL/Fe3O4@ ZIF-8 magnetic nanocomposite on infected bone defect

Yun Xiao1,2 ,  Yi Ding3 ,  Jun Qiu4 ,  Xiaonan Zhang5 ,  Yanzhen Zheng5 ,  Chong Huang2 ,  Lu Zhao2 ,  Zihao Tang2 ,  Yuanli Chen2 ,  Yiwen Liu2 ,  Kezhen Zhao2 ,  Kai Guo2 ,  Li Jing2 ,  Mingchao Ding2 ,  Chunlin Zong2* ,  Jiankang He3* ,  Lei Tian2*
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1 School of Stomatology, Jiamusi University, Jiamusi, Heilongjiang, China
2 State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, China
3 State Key Laboratory for Manufacturing Systems Engineering, National Medical Products Administration (NMPA) Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi’an Jiaotong University, Xi’an, Shaanxi, China
4 State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, China
5 Frontier Institute of Science and Technology (FIST), State Key Laboratory of Mechanical Behavior for Materials, MOE Key Laboratory for Nonequilibrium Synthesis of Condensed Matter, Xi’an Key Laboratory of Sustainable Energy and Materials Chemistry, School of Chemistry, Xi’an Jiaotong University, Xi’an, Shaanxi, China
IJB 2024, 10(4), 2271 https://doi.org/10.36922/ijb.2271
Submitted: 17 November 2023 | Accepted: 5 February 2024 | Published: 27 March 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

 Treatment of large bone defects remains a clinical challenge, especially for defects compounded by infection. It is essential to develop dual functional therapeutic systems that inhibit bacterial growth and promote bone regeneration for infected bone defects treatment. However, the ideal bone substitute biomaterials to repair infected bone defects remain scarce. In this study, we fabricated a novel magnetic nanocomposite with polycaprolactone (PCL)/Fe3O4@zinc-based imidazole zeolite framework-8 (ZIF-8) through three-dimensional (3D) printing technology. The biomaterial characterization, biocompatibility, antibacterial activity, and osteogenic ability of the 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite were systematically investigated in vivo and in vitro. The 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite scaffolds showed a square porous grid structure with rough surface, thermal stability, superparamagnetic character, and slow release of Zn2+. The elevation of Fe3O4@ZIF-8 concentration increased surface roughness and porosity, improved the mechanical properties, and enhanced the saturation magnetization of PCL/Fe3O4@ZIF-8 scaffolds. The PCL/Fe3O4@ZIF-8 scaffolds possessed good biocompatibility and promoted the proliferation and adhesion of rat bone marrow mesenchymal stem cells (BMSCs). The PCL/Fe3O4@ZIF-8 scaffolds also upregulated the expression of osteogenic-related genes and proteins and promoted the osteogenic differentiation of BMSCs by activating the Wnt/β-catenin signaling pathway. Furthermore, the scaffolds showed excellent antibacterial activities, which increased with increasing Fe3O4@ ZIF-8 nanoparticle concentration. In vivo experiments proved that the scaffolds eliminated infection and promoted new bone formation in infected bone defect. Given excellent osteogenic and antibacterial activities, the 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite scaffolds could serve as novel materials for the treatment of infected bone defects.

Keywords
Infected bone defect
3D printing
Fe3O4 nanoparticles
Zinc-based imidazole zeolite framework-8
Anti¬bacterial activity
Bone regeneration
Funding
This study was supported by the National Natural Science Foundation of China (grant number: 81903249), the Natural Science Basic Research Program of Shaanxi province (grant number: 2024SF-YBXM-264, 2022JZ- 50, 2023-YBSF-291), and Xi’an Science and Technology Program (grant number: 22YXYJ0156). The funders had no role in the design or outcome of this study.
Conflict of interest
The authors declare no conflicts of interest.
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