AccScience Publishing / IJB / Volume 9 / Issue 5 / DOI: 10.18063/ijb.768

A sturgeon cartilage extracellular matrix-derived bioactive bioink for tissue engineering applications

Xiaolin Meng1 Zheng Zhou2* Xin Chen1 Feng Ren3* Wenxiang Zhu1 Shuai Zhu1 Hairong Liu1*
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1 College of Materials Science and Engineering, Hunan University, Changsha 410082, PR China
2 College of Biology, Hunan University, Changsha 410082, PR China
3 Department of Geriatric Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, PR China
Submitted: 28 March 2023 | Accepted: 10 April 2023 | Published: 6 June 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( )

Three-dimensional (3D) bioprinting provides a promising strategy for tissue and organ engineering, and extracellular matrix (ECM)-derived bioinks greatly facilitate its applications in these areas. Decellularized sturgeon cartilage ECM (dSC-ECM)- derived bioinks for cartilage tissue engineering were fabricated with methacrylatemodified dSC-ECM (dSC-ECMMA) and sericin methacrylate (SerMA), which optimizedthe mechanical properties of their solidified hydrogels.dSC-ECM induces chondrocytes to form cell clusters and subsequently reduces their proliferation, but the proliferation of encapsulated chondrocytes was normal in solidified dSCECM-5 bioink samples, which contain 5 mg/mL dSC-ECMMA. Hence, this bioink was selected for further investigation. Lyophilized dSC-ECM-5 hydrogels showed connected pore microstructure, which is suitable for cell migration and nutrients transportation. ThisdSC-ECM-5 bioink exhibited high fidelity and good printability by testing via a 3D bioprinting system, and the chondrocytes loaded in printed hydrogel products were viable and able to grow, following incubation, in the cell culture medium. Solidified dSC-ECM-5 and SerMA bioinks loaded with chondrocytes were subcutaneously implanted into nude mice for 4 weeks to test the suitability of the bioink for cartilage tissue engineering. Compared to the SerMA bioink, the dSCECM-5 bioink significantly enhanced cartilage tissue regeneration and maturation in vivo, suggesting the potential of this bioink to be applied in cartilage tissue engineering in the future.

3D bioprinting
ECM-derived bioink
Cartilage tissue engineering
Cartilage regeneration

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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing