AccScience Publishing / IJB / Volume 10 / Issue 4 / DOI: 10.36922/ijb.3784
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RESEARCH ARTICLE

3D-printed variable stiffness tissue scaffolds for potential meniscus repair

Caroline A. Murphy1 ,  Aleksandra Serafin1,2 ,  Ibrahim Fatih Cengiz3,4 ,  Rui L. Reis3,4 ,  Joaquim Miguel Oliveira3,4 ,  Maurice N. Collins1,2,5*
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1 Stokes Laboratories, Bernal Institute, School of Engineering, University of Limerick, Limerick, Ireland
2 Health Research Institute, University of Limerick, Limerick, V94 T9PX, Ireland
3 3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal
4 ICVS/3B’s–PT Government Associate Laboratory, Guimarães, Portugal
5 SFI Centre for Advanced Materials and BioEngineering Research, D02 PN40 Dublin, Ireland
IJB 2024, 10(4), 3784 https://doi.org/10.36922/ijb.3784
Submitted: 29 May 2024 | Accepted: 9 July 2024 | Published: 12 August 2024
(This article belongs to the Special Issue Biomimetic and Bioinspired Printed Structures)
© 2024 by the Author(s).. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

The treatment of meniscus injuries has recently shifted toward the field of tissue engineering (TE). In this work, bovine menisci were characterized, and the regionally-dependent mechanical properties were analyzed. Three-dimensional (3D) printing technology was employed to produce a scaffold that mimicked the mechanical properties of the meniscus. A polycaprolactone (PCL) meniscus scaffold was 3D printed, allowing for the deposition of fibers mimicking the internal architecture of the native meniscus, while achieving regional and variable mechanical stiffness, varying from 2.74 to 0.88 MPa. The PCL scaffold was infiltrated with extracellular matrix (ECM)-like hydrogels composed of gelatin methacrylate (GelMA) and glycosaminoglycans (GAGs), such as hyaluronic acid (HA) and chondroitin sulfate (CS), and subsequently freeze-dried. Human mesenchymal stem cells were seeded onto the scaffolds, and the infiltrated cells were observed to produce ECM components of the native meniscus. Collagen and GAGs production was successfully established. The synthesis of a new matrix reportedly enhances the mechanical properties of the hydrogel over time. Additionally, the circumferential PCL fibers within the scaffold guided the newly synthesized matrix, facilitating replication of the native tissue structure. These results indicate that the ECM-infiltrated 3D-printed PCL scaffold is a promising solution for meniscus repair.

Graphical abstract
Keywords
3D printing
Biomaterials
Biomechanics
Hydrogels
Meniscus
Repair
Scaffolds
Funding
The authors would like to thank the funding provided by the Irish Research Council (Project ID: EPSPG/2015/93) in partnership with Johnson & Johnson and the Government of Ireland (Postdoctoral Fellowship Project ID: GOIPD/2023/1431). I.F.C. thanks the Portuguese Foundation for Science and Technology (FCT) and acknowledges the FCT distinction attributed to I.F.C. under the Estímulo ao Emprego Científico program (2021.01969. CEECIND) (https://doi.org/10.54499/2021.01969. CEECIND/CP1664/CT0017). This study received financial support from the European Commission-funded Oncoscreen project (Grant agreement ID: 101097036). The authors also thank the FEDER program for funds provided under the JUSTHera project (NORTE-01-0145- FEDER-000055) and the 0624_2IQBIONEURO_6_E project (inter-regional cooperation program VA Spain- Portugal POCTEP 2014–2020). The authors thank the financial support provided under the project “HEALTH-UNORTE: Setting-up biobanks and regenerative medicine strategies to boost research in cardiovascular, musculoskeletal, neurological, oncological, immunological and infectious diseases” (NORTE-01- 0145-FEDER-000039), funded by the NORTE2020 Program, Norte Portugal Regional Coordination and Development Commission (CCDR-N). The authors also thank FCT for funding LA ICVS/3B: (i) “Base” funding (https://doi.org/10.54499/UIDB/50026/2020); (ii) “Programático” funding: (https://doi.org/10.54499/ UIDP/50026/2020); and iii) “Complementar - LA” funding: (https://doi.org/10.54499/LA/P/0050/2020).
Conflict of interest
The authors declare no conflicts of interest.
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