AccScience Publishing / IJB / Volume 9 / Issue 6 / DOI: 10.36922/ijb.0985
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RESEARCH ARTICLE

3D bioprinting-based single liver tumor spheroid analysis for aflatoxin B1-induced drug-resistant cancer cell

Viet Phuong Cao1 ,  Sera Hong1 ,  Joon Myong Song1 *
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1 College of Pharmacy, Seoul National University, Seoul 08826, South Korea
IJB 2023, 9(6), 0985 https://doi.org/10.36922/ijb.0985
Submitted: 24 May 2023 | Accepted: 1 July 2023 | Published: 18 August 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1

Keywords
3D bioprinting
Single tumor spheroid imaging
Aflatoxin B1
Drug-resistant cancer cell
Funding
This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) (No. 2018M3A7B4071235, 2023R1A2C3006991, and RS-2023-00218543).
Conflict of interest
The authors declare no conflicts of interests.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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