AccScience Publishing / JCBP / Volume 2 / Issue 3 / DOI: 10.36922/jcbp.2381
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ORIGINAL RESEARCH ARTICLE

Unraveling the biomolecular effects of Mongolian mind-body interactive psychotherapy on psoriasis: An exosome proteomic analysis

Haorile Chagan-Yasutan1,2* ,  Nagongbilige He1,3* ,  Sarnai Arlud1 ,  Siriguleng Wuyun1 ,  Ruiming Gao1 ,  Wenfeng Bao3 ,  Rihan Wu4 ,  Qiqige Gerile4 ,  Jiletu Xin4 ,  Toshio Hattori2,5
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1 Department of Mongolian Psychosomatic Medicine, Inner Mongolia International Mongolian Medicine Hospital, Hohhot, China
2 Research Institute of Health and Welfare, Kibi International University, 8-Iga-machi, Takahashi, Okayama, Japan
3 The Inner Mongolia Institute of Chinese and Mongolian Medicine, Hohhot, China
4 Ordos Mongolian Medical Hospital, Kangbashi District, Ordos, Inner Mongolia Autonomous Region, China
5 Shizuoka Graduate University of Public Health, 4-27-2 Kita Ando Aoi-ku, Shizuoka City, Japan
JCBP 2024, 2(3), 2381 https://doi.org/10.36922/jcbp.2381
Submitted: 6 December 2023 | Accepted: 24 April 2024 | Published: 24 May 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Psoriasis is a chronic inflammatory skin disease etiologically related to stress. Mongolian mind-body interactive psychotherapy (MMIP) is a group psychotherapy that integrates traditional Mongolian medicine theory with modern psychology. Exosomes are nanovesicles that carry biomolecules such as proteins, and the lipid bilayer protects cargo from degeneration. The present study was conducted to discern the effects of MMIP on psoriasis from a biomolecular perspective, particularly by performing an exosome proteomic analysis. Plasma exosomes were extracted from 15 samples, derived from psoriasis patients (n = 5, before and after MMIP) and healthy controls (HCs; n = 5). All patients had spent 60 days engaging with MMIP. Proteomic analysis was conducted using high-throughput mass spectrometry, and the differentially expressed proteins (DEPs) were analyzed. Further, validation test was done in serum samples of psoriasis (n = 56) and HC (n = 29). After the MMIP, the psoriasis area and severity index improved by 75%. Compared to HCs, psoriasis patients demonstrated alterations in 41 DEPs, with significant involvement of ribosome and apelin/APJ pathway. The recovery of psoriasis following the therapy was found to be associated with significant alterations in 16 DEPs, involving pathways of Fc gamma-mediated receptor phagocytosis, tight junction, and vascular smooth muscle contraction. Notably, one of the immunoglobulins that were reduced in psoriasis was significantly elevated after the MMIP. Validation results showed that the levels of serum elafin were higher in psoriasis than in HC but significantly decreased after MMIP. In conclusion, this study demonstrated that MMIP has a significant influence on the profiles of immunoglobulins and inflammatory molecules, as well as several pathways participating in the psoriasis recovery, providing insights into the pathophysiology of psoriasis and possible potential therapeutic targets of the disease.

Keywords
Psoriasis
Plasma exosome
Proteomic analysis
Mongolian mind-body interactive psychotherapy
Stress
Group psychotherapy
Biomarker
Inflammation
Funding
This research was supported by the Natural Science Foundation of Inner Mongolia Autonomous Region (2021MS08101), and partly supported by the Science and Technology Major Project of the Inner Mongolia Autonomous Region (NMGZDZX2017) and Mongolian Psychosomatic Medicine Innovation Talent Team Project of the Ordos City (2021).
Conflict of interest
The authors declare no conflicts of interest.
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