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ORIGINAL RESEARCH ARTICLE

Evaluation of antimicrobial, analgesic, and hypoglycemic activities of Commelina diffusa (Commelinaceae)

Tasnia Islam Anika1 ,  Md. Monirul Islam2 ,  Mohammad Shawkat Ali1*
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1 Department of Clinical Pharmacy & Pharmacology, University of Dhaka, Dhaka, Bangladesh
2 Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh
IMO, 025270030 https://doi.org/10.36922/IMO025270030
Received: 4 July 2025 | Revised: 19 July 2025 | Accepted: 1 August 2025 | Published online: 18 August 2025
© 2025 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC-by the license) ( https://creativecommons.org/licenses/by-nc/4.0/ )
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Abstract

Commelina diffusa, also known as the climbing dayflower or spreading dayflower, is an herbaceous plant from the Commelinaceae family, found throughout tropical regions, including Bangladesh. The crude methanol extract and different fractions of C. diffusa were evaluated for their antimicrobial, analgesic, and hypoglycemic activities. The whole plant was extracted with methanol by the cold extraction method. The concentrated extract was then partitioned into petroleum ether- and chloroform-soluble fractions. The antimicrobial test was performed using the disc diffusion method. The analgesic effects were evaluated through both writhing and tail-flick tests at doses of 100 and 200 mg/kg body weight. The oral glucose tolerance test (OGTT) was performed to observe the hypoglycemic effect. The chloroform and methanol soluble fractions showed potent antimicrobial activity against Gram-positive bacteria and fungi. Statistical evaluation of the tail-flick test confirmed that the chloroform soluble fraction (100 mg/kg body weight) of C. diffusa had a significant amount of central analgesic activity (p<0.001). The petroleum ether soluble fraction showed significant central analgesic activity only at higher doses (p <0.01; 200 mg/kg body weight). The acetic acid-induced writhing test also confirmed the peripheral analgesic activity of the samples. The maximum inhibition was noted for the chloroform soluble fraction (64.56%), followed by crude methanolic extract (56.96%) and petroleum ether soluble fraction (53.16%). However, all the extracts showed no significant hypoglycemic activity in the OGTT. This observation, derived from an acute model of non-diabetic animals, does not preclude the possibility of antidiabetic effects in disease-related conditions. Further investigation is warranted to explore the specific metabolites and their pharmacological activities in relevant disease models.

Keywords
Commelina diffusa
Methanol extract
Antimicrobial activity
Hypoglycemic activity
Analgesic activity
Funding
None.
Conflict of interest
The authors declare that they have no competing interests.
References
  1. Khan MAA, Islam MT, Sadhu SK. Evaluation of phytochemical and antimicrobial properties of Commelina diffusa Burm. f. Orient Pharm Exp Med. 2011;11(4):235-241. doi: 10.1007/S13596-011-0028-0

 

  1. Isaac P, Brathwaite R, Isaac W. Commelina Species -A Review of its Weed Status and Possibilities for Alternative Weed Management in the Tropics; 2007. Available from: AQ1 https:// www.semanticscholar.org/paper/commelina-species-a-review-of-its-weed-status-and-isaac-brathwaite/4ed0bef1c0 ff432684c9c7c9239d8aa1b46d8173 [Last accessed on 2025 May 14].

 

  1. Rahman MM, Mannan MA, Nijhu RS, Khatun A. Traditional uses, phytochemistry and pharmacology of Commelina diffusa Burm: An updated systematic review. J Pharmacogn Phytochem. 2021;10(4):53-59.

 

  1. Hasan M, Islam MM, Rahman MM. A review on structure - activity relationship of antimicrobial peptide magainin 2. Dhaka Univ J Pharm Sci. 2022;20(3):427-434. doi: 10.3329/dujps.v20i3.59806

 

  1. Hasan M, Islam M, Shorna M, Sarder M. Plant antimicrobial peptides in Bangladesh: Sources, extraction, purification and characterization. Jagannath Univ J Life Earth Sci. 2021;6(1):95-110.

 

  1. Rivière C. Natural products as antimicrobial agents: From extraction to therapeutic applications. Molecules. 2025;30(11):2393. doi: 10.3390/MOLECULES30112393

 

  1. Guglielmi P, Pontecorvi V, Rotondi G. Natural compounds and extracts as novel antimicrobial agents. Expert Opin Ther Pat. 2020;30(12):949-962. doi: 10.1080/13543776.2020.1853101

 

  1. Islam MM, Manik IN, Zobayed A, Noor F. A review on medicinal properties some commonly used culinary agents of Bangladesh. J Med Plants Stud. 2021;9(3):111-117.

 

  1. Hewitt DJ, Hargreaves RJ, Curtis SP, Michelson D. Challenges in analgesic drug development. Clin Pharmacol Ther. 2009;86(4):447-450. doi: 10.1038/CLPT.2009.161

 

  1. Chandel P, Rawal RK, Kaur R. Natural products and their derivatives as cyclooxygenase-2 inhibitors. Future Med Chem. 2018;10(20):2471-2492. doi: 10.4155/FMC-2018-0120

 

  1. Sapra A, Bhandari P. Diabetes. StatPearls -NCBI Bookshelf; 2023. Available from: https://www.ncbi.nlm.nih.gov/books/ NBK551501 [Last accessed on 2025 May 14].

 

  1. Salehi B, Ata A, Kumar NVA, et al. Antidiabetic potential of medicinal plants and their active components. Biomolecules. 2019;9(10):551. doi: 10.3390/biom9100551

 

  1. Prima AA, Ahmed R, Faruk A, Zafroon, Z, Dash PR. Pharmacological importance of Commelina diffusa (Commelinaceae): A review. Int J Pharm Sci Res. 2019;5(1):1-5.

 

  1. Vu DL, Van Anh Nguyen T, Nguyen TD, Dang VH, Le HD, Nguyen XT. Anti-diabetic effect of major compounds from Commelina diffusa. Rev Bras Farmacogn. 2023;33(3):657-661. doi: 10.1007/s43450-023-00394-7

 

  1. Emran T Bin, Rahman MA, Uddin MMN, et al. Effects of organic extracts and their different fractions of five Bangladeshi plants on in vitro thrombolysis. BMC Complement Altern Med. 2015;15(1):128. doi: 10.1186/S12906-015-0643-2

 

  1. Hindler JF, Munro S. Antimicrobial susceptibility testing. In: Clinical Microbiology Procedures Handbook. 3rd ed. United States: ASM Press; 2024. p. 2-3. doi: 10.1128/9781555817435.ch5

 

  1. Jain SN, Sampath A. Evaluation of in vitro synergy between ampicillin and kanamycin against Staphylococcus aureus evaluation of in vitro synergy between ampicillin and kanamycin against Staphylococcus aureus. J Drug Deliv Ther. 2012;2(4):144-146.

 

  1. Yemitan OK, Adeyemi OO. Mechanistic assessment of the analgesic, anti-inflammatory and antipyretic actions of Dalbergia saxatilis in animal models. Pharm Biol. 2017;55(1):898-905. doi: 10.1080/13880209.2017.1283706

 

  1. Casarrubea M, Faulisi F, Magnusson MS, Crescimanno G. The effects of morphine on the temporal structure of Wistar rat behavioral response to pain in hot-plate. Psychopharmacology (Berl). 2016;233(15-16):2891-2900. doi: 10.1007/s00213-016-4334-6

 

  1. Ojewole JAO. Antinociceptive, anti-inflammatory and antidiabetic effects of Bryophyllum pinnatum (Crassulaceae) leaf aqueous extract. J Ethnopharmacol. 2005;99(1):13-19. doi: 10.1016/j.jep.2005.01.025

 

  1. Ahmed M, Shikha HA, Sadhu SK, Rahman MT, Datta BK. Analgesic, diuretic, and anti-inflammatory principle from Scoparia dulcis. Pharmazie. 2001;56(8):657-660.

 

  1. Stumvoll M, Mitrakou A, Pimenta W, et al. Use of the oral glucose tolerance test to assess insulin release and insulin sensitivity. Diabetes Care. 2000;23(3):295-301. doi: 10.2337/diacare.23.3.295

 

  1. Tesfaye A, Makonnen E, Gedamu S. Hypoglycemic and antihyperglycemic activity of aqueous extract of Justicia schimperiana leaves in normal and streptozotocin-induced diabetic mice. Int J Pharma Sci Res. 2016;7(2):107-113.

 

  1. Chaimum-Aom N, Chomko S, Talubmook C. Toxicology and oral glucose tolerance test (OGTT) of Thai medicinal plant used for diabetes controls, Phyllanthus acidus L. (Euphorbiaceae). Pharmacogn J. 2017;9(1):58-61. doi: 10.5530/pj.2017.1.11

 

  1. Yahiya YS, Temeche AM, Delisho FD, Abrar KA. In vitro antibacterial, antioxidant and XRF analysis of Commelina diffusa burm. F. plant extracts. J Dis Med Plants. 2024;10(3):40-51. doi: 10.11648/J.JDMP.20241003.11

 

  1. Islam MM, Rahman M, Asaduzzaman M, Ali MS. Antioxidant, Analgesic and CNS depressant activities of Commelina diffusa Burm. f. Dhaka Univ J Pharm Sci. 2021;20(2):159-166. doi: 10.3329/DUJPS.V20I2.57166

 

  1. Ahmad I, Hasan M, Bhowmik DR, et al. Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ. Heliyon. 2025;11(2):e41949. doi: 10.1016/j.heliyon.2025.e41949

 

  1. Khan AA, Diogenes A. Pharmacological management of acute endodontic pain. Drugs. 2021;81(14):1627-1643. doi: 10.1007/S40265-021-01564-4

 

  1. Hasan SMR, Hossain MM, Akter R, et al. Analgesic activity of the different fractions of the aerial parts of Commelina benghalensis Linn. Int J Pharmacol. 2010;6(1):63-67. doi: 10.3923/ijp.2010.63.67

 

  1. Hossain F, Saha S, Nasrin S, Adhikari S. Analgesic and anti-infammatory activity of Commelina benghalensis Linn. Turk J Pharm Sci. 2014;11(1):25-32.

 

  1. Hisham AA, Islam MM, Shah SMT, Al Mamun MA, Hossain MS. Bioactive compounds from lactic acid bacterial (LAB) fermented products modulate metabolic parameters and mRNA expression of adipogenic and inflammatory genes in obese mice. ACS Food Sci Technol. 2025;5(4):1710- 1720. doi: 10.1021/acsfoodscitech.5c00087

 

  1. Gurjar HPS, Irchhaiya R, Vermas A. Antidiabetic activity and phytochemical investigation on the whole plant of Commelina benghalensis linn. in male albino rat. J Drug Deliv Ther. 2016;6(2):26-29. doi: 10.22270/jddt.v6i2.1200

 

  1. Ghani A. Medicinal plants of Bangladesh: Chemical Constituents and Uses. Dhaka: Asiatic Society of Bangladesh; 2003.

 

  1. Manik MIN, Ali MH, Ray MN, et al. A comprehensive review on medicinal values and health benefits of spices and condiments commonly used in the Indian sub-continent. Res J Pharmacogn Phytochem. 2022;14(1):11-18. doi: 10.52711/0975-4385.2022.00003

 

  1. Isaac WA, Gao Z, Li M. Managing Commelina species: Prospects and limitations. In: InTech eBooks. London: IntecOpen; 2013. doi: 10.5772/55842

 

  1. Nasrin M, Afroz F, Sharmin S, et al. Cytotoxic, antimicrobial and antioxidant properties of Commelina diffusa Burm. F. Pharmacol Pharm. 2019;10(2):82-93. doi: 10.4236/pp.2019.102007

 

  1. Li X, Zuo S, Wang B, Zhang K, Wang Y. Antimicrobial mechanisms and clinical application prospects of antimicrobial peptides. Molecules. 2022;27(9):2675. doi: 10.3390/molecules27092675

 

  1. Rozwandowicz M, Brouwer MSM, Fischer J, et al. Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae. J Antimicrob Chemother. 2017;73(5):1121-1137. doi: 10.1093/jac/dkx488

 

  1. Levy SB, Bonnie M. Antibacterial resistance worldwide: Causes, challenges and responses. Nat Med. 2004;10(12 Suppl):S122-S129. doi: 10.1038/NM1145

 

  1. Arnab MKH, Hasan M, Islam MM. An Insight into the structure-activity relationship of antimicrobial peptide brevinin. Jordan J Pharm Sci. 2023;16(4):815-829. doi: 10.35516/jjps.v16i4.1327

 

  1. Islam MM, Asif F, Zaman SU, Arnab MKH, Rahman MM, Hasan M. Effect of charge on the antimicrobial activity of alpha-helical amphibian antimicrobial peptide. Curr Res Microb Sci. 2023;4:100182. doi: 10.1016/j.crmicr.2023.100182

 

  1. Read AF, Woods RJ. Antibiotic resistance management. Evol Med Public Health. 2014;2014(1):147-147. doi: 10.1093/emph/eou024

 

  1. Larsson DGJ, Flach CF. Antibiotic resistance in the environment. Nat Rev Microbiol. 2022;20(5):257-269. doi: 10.1038/S41579-021-00649-x

 

  1. Darby EM, Trampari E, Siasat P, et al. Molecular mechanisms of antibiotic resistance revisited. Nat Rev Microbiol. 2023;21(5):280-295. doi: 10.1038/S41579-022-00820-Y

 

  1. Manik MIN, Ali MH, Islam MM, et al. In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the Ampelocissus barbata (Wall.) Leaves. Biomed Pharmacol J. 2022;15(2):911-923. doi: 10.13005/bpj/2426

 

  1. Gonçalves S, Fernandes L, Caramelo A, Martins M, Rodrigues T, Matos RS. Soothing the Itch: The role of medicinal plants in alleviating pruritus in palliative care. Plants. 2024;13(24):3515. doi: 10.3390/plants13243515

 

  1. Cui J, Jia J. Natural COX-2 inhibitors as promising anti-inflammatory agents: An update. Curr Med Chem. 2020;28(18):3622-3646. doi: 10.2174/0929867327999200917150939
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