Musculoskeletal tissues, including bone, cartilage, and tendon, are essential for body movement and function, yet they often face limited regenerative capacity following injury or degeneration. Bioprinting technologies offer a transformative approach to musculoskeletal tissue engineering and regenerative medicine, by enabling precise cell and biomaterial placement and replicating complex native structures, leading to more reproducible and effective outcomes. This Special Issue invites original contributions that explore the potential of bioprinting technologies to advance tissue engineering and regeneration of musculoskeletal tissues. We welcome studies on bioinks that replicate the extracellular matrix of these tissues, bioprinting techniques aimed at tissue repair and regeneration, and the development of bioprinted personalized implants or in vitro models for drug testing or disease modeling. This Special Issue aims to highlight both current advancements and future challenges in the field.