Bioprinting employs advanced strategies to replicate the complex structures and functions of tissues and organs. Researchers have modeled various tissues, organs, and physiological/pathological processes through interdisciplinary collaboration spanning engineering, radiology, pathology, biophysics, and developmental biology. These efforts have driven the development of biotechnologies and materials currently undergoing preclinical and clinical examinations or further study. However, challenges remain, including the limited functionality and selections of biomaterials, insufficient print resolution, and the complexity of regulating biological processes in tissue maturation and disease progression. This special issue, Multidisciplinary Efforts in Bioprinting, highlights recent advancements in 3D bioprinting and their role in overcoming these obstacles.