This special issue aims to showcase the latest advancements in bioprinting technologies that address critical and persistent challenges in tissue engineering and regenerative medicine. With a focus on functional materials that enhance cell viability, promote vascularization, and improve tissue growth within 3D-printed scaffolds, the issue delves into cutting-edge approaches for regenerating complex tissues and organs. By incorporating biofunctional properties such as mechanical strength, controlled degradation, oxygenation, and biocompatibility, these materials offer innovative solutions to long-standing issues like nutrient diffusion, oxygen supply, and mechanical stability, particularly in large or avascular tissue constructs. This collection of original research articles and comprehensive reviews will highlight innovative biomaterial designs, synthesis techniques, and advanced bioprinting methods. It demonstrates how functional biomaterials are revolutionizing tissue engineering outcomes and paving the way for future advancements in regenerative medicine and clinical applications.