Prior spaceflight and simulated low gravity studies have provided unique insights into cellular changes that are induced in the space environment. Microgravity enables the biofabrication of three-dimensional (3D) structures in more complex geometries without the need for structural support and enables the use of less viscous bioinks. Manuscripts are requested that deals with the application of 3D bioprinting techniques in microgravity, focusing on tissue chips and organoids, choice of biomaterials, and their implications for biomedical research in space. Research gaps in this area include the need to understand the fundamental properties of cells and proteins in microgravity and how to better design bioprinting techniques and biomaterials for higher quality and function for clinical applications.