Bacteriorhodopsin is a promising photosensitive material with applications in energy conversion, biosensors, and optoelectronic devices due to its bio-sourced origin and photoelectrical properties. Despite advancements in recent years, the integration of bacteriorhodopsin-based devices necessitates the use of materials with little biocompatibility and fabrication techniques with restricted customizability, limiting potential applications such as optocontrol of cell behavior, implants with 3D patterning for retinal disease treatment, and light-sensitive cell robots. To solve this limitation, this study presents a novel approach by embedding bacteriorhodopsin into a hydrogel matrix utilizing an extrusion-based and 3D bioprinting technique, showcasing its light-sensitive characteristics within a fully biocompatible construct. The hydrogel comprising gelatin and sodium alginate offers excellent printability for generating structured designs with versatile patterns. Photoelectrical properties of the fabricated bacteriorhodopsin-embedded hydrogel construct, such as differential response, light intensity sensitivity, and bacteriorhodopsin concentration sensitivity, are identified through electrochemical characterization. The temporal and spatial pattern recognition ability, based on the photoelectrical characteristics, is demonstrated through modulated light illumination and different patterns of printed hydrogel construct. The pattern recognition ability was then applied for the reconstruction of images containing different Latin letters. This research presents a novel method for the fabrication of patterned hydrogel constructs with high biocompatibility and distinctive light-responsive properties, expanding the potential applications of bacteriorhodopsin in bio-related scenarios.