AccScience Publishing / IJB / Online First / DOI: 10.36922/ijb.2021
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RESEARCH ARTICLE

A new solution for in situ monitoring of shape fidelity in extrusion-based bioprinting via thermal imaging

Simone Giovanni Gugliandolo1,2 ,  Egon Prioglio1 ,  Davide Moscatelli2 ,  Bianca Maria Colosimo1*
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1 Department of Mechanical Engineering, Politecnico di Milano, Via La Masa, 1, 20156, Milano, Italy
2 Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, Piazza Leonardo da Vinci, 32, 20133, Milano, Italy
IJB 2024, 10(3), 2021 https://doi.org/10.36922/ijb.2021
Submitted: 12 October 2023 | Accepted: 28 December 2023 | Published: 22 March 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Bioprinting is an interdisciplinary study field, where additive manufacturing is combined with tissue engineering and material sciences. The ever-increasing need for personalized medicine fueled interest in the possibility of using this technique to reproduce biological tissues, allowing bioprinting to establish itself as one of the most promising approach in biomedical research. Producing bioconstructs that resemble living tissues is a very complex and multi-step procedure. Given the complexity of the processes involved, the literature still lacks robust solutions for monitoring the bioprinted construct quality, especially in situ and in-line. Here, a novel non-destructive approach for monitoring the geometries of bioprinted constructs based on infrared (IR) imaging is proposed. Besides the intuitive use of IR information to gain insight on the temperature signature, we propose IR video imaging as a viable solution to overcome traditional problems of visible-range imaging for geometry reconstruction with transparent bioinks, especially when precise information on the last printed layer only is required. The results obtained show a significant new direction for in-line monitoring of bioprinting processes.

Keywords
Additive manufacturing
3D bioprinting
Thermal imaging
Monitoring
Funding
This research was partially funded by the European Commission under the “HORIZON-CL4-2021-DIGITAL-EMERGING-01 project BioProS - Biointelligent Production Sensor to Measure Viral Activity” (grant agreement no. 101070120, 2022–2026).
Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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