AccScience Publishing / IJB / Volume 9 / Issue 2 / DOI: 10.18063/ijb.v9i2.671
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RESEARCH ARTICLE

Preparation and characterization of 3D-printed antibacterial hydrogel with benzyl isothiocyanate

Yunxia Liang1† Bimal Chitrakar2† Zhenbin Liu1 Xujia Ming1 Dan Xu1 Haizhen Mo1 Chunyang , Shi1 Xiaolin Zhu1 Liangbin Hu1* Hongbo Li1
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1 School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an, 710021, China
2 College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, Hebei, China
Submitted: 9 September 2022 | Accepted: 8 November 2022 | Published: 17 January 2023
(This article belongs to the Special Issue Related to 3D printing technology and materials)
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Benzyl isothiocyanate (BITC) is an isothiocyanate of plant origin, especially the mustard family, which has good antibacterial properties. However, its applications are challenging due to its poor water solubility and chemical instability. We used food hydrocolloids, including xanthan gum, locust bean gum, konjac glucomannan, and carrageenan as three-dimensional (3D)-printing food ink base and successfully prepared 3D-printed BITC antibacterial hydrogel (BITC-XLKC-Gel). The characterization and fabrication procedure of BITC-XLKC-Gel was studied. The results show that BITC-XLKC-Gel hydrogel has better mechanical properties by lowfield nuclear magnetic resonance (LF-NMR), mechanical properties, and rheometer analysis. The strain rate of BITC-XLKC-Gel hydrogel is 76.5%, which is better than that of human skin. Scanning electron microscope (SEM) analysis showed that BITC-XLKC-Gel has uniform pore size and provides a good carrier environment for BITC carriers. In addition, BITC-XLKC-Gel has good 3D-printing performance, and 3D printing can be used for customizing patterns. Finally, inhibition zone analysis showed that the BITC-XLKC-Gel added with 0.6% BITC had strong antibacterial activity against Staphylococcus aureus and the BITC-XLKC-Gel added with 0.4% BITC had strong antibacterial activity against Escherichia coli. Antibacterial wound dressing has always been considered essential in burn wound healing. In experiments that simulated burn infection, BITC-XLKC-Gel showed good antimicrobial activity against methicillin-resistant S. aureus. BITC-XLKC-Gel is a good 3D-printing food ink attributed to strong plasticity, high safety profile, and good antibacterial performance and has great application prospects.

Keywords
Antibacterial hydrogel
Benzyl isothiocyanate
3D printing
Rheological properties
Staphylococcus aureus
Escherichia coli
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing