AccScience Publishing / AN / Volume 2 / Issue 3 / DOI: 10.36922/an.0872

Distinct behavioral effects of short-term voluntary running in phosphatase and tensin homolog deleted on chromosome 10 neuronal haploinsufficient mice

Diana Zukas Andreotti1 Natália Prudente de Mello1 Amanda Galvão Paixão1 Cristoforo Scavone2 Ana Maria Orellana1,2 Elisa Mitiko Kawamoto1*
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1 Laboratory of Molecular and Functional Neurobiology, Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
2 Laboratory of Molecular Neuropharmacology, Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
Advanced Neurology 2023, 2(3), 0872
Submitted: 27 April 2023 | Accepted: 10 July 2023 | Published: 2 August 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( )

Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a tumor suppressor with functions related to its phosphatase activity. PTEN plays various roles, such as cell proliferation, survival, and migration and is involved in neurogenesis and synaptic plasticity in the central nervous system. It has been reported that running could have protective effects against the aging process and neurodegenerative diseases. Therefore, we aimed to evaluate the effects of physical exercise on behavioral and biochemical aspects of PTEN-conditioned knockout female mice. We observed that 10 days of voluntary running positively affected fear memory but caused no changes in anxiety-like behavior. However, it was unable to counteract the social recognition memory deficit in PTEN neuronal haploinsufficient mice. In terms of biochemical aspects, we observed that short-term running reduced S6 phosphorylation in PTEN heterozygous mice and PTEN protein expression independent of the genotype. In addition, PTEN heterozygous mice presented reduced N-methyl-D-aspartate subunit NR1 protein expression. Our results regarding decreased S6 phosphorylation in HT mice suggest that short-term voluntary running could have induced a protective effect in reducing dysregulated cell growth, possibly related to the downregulation of tumor suppressor expression/activity such as PTEN. Moreover, running induced distinct behavioral effects in PTEN-conditioned knockout mice.

Fundação de Amparo à Pesquisa do Estado de São Paulo
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
MSc fellowship from FAPESP
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Conflict of interest
The authors declare no competing interests.
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