AccScience Publishing / TD / Online First / DOI: 10.36922/td.3670
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ORIGINAL RESEARCH ARTICLE

Investigating long noncoding RNA HA117 and its possible regulatory network in osteosarcoma

Hongxia Chen1† ,  Zhiyong Cheng2† ,  Wei Wang3 ,  Zhenhua Zhuang4 ,  Xiaoping Huang3 ,  Ning Wang3*
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1 Department of Hematology, Chong Qing University Three Gorges Hospital, Chongqing, China
2 Orthopedic Trauma Unit, Chong Qing University Three Gorges Hospital, Chongqing, China
3 Department of Oncology, Chong Qing University Three Gorges Hospital, Chongqing, China
4 Chengdu Life Baseline Technology CO., LTD, Chengdu, Sichuan, China
Tumor Discovery, 3670 https://doi.org/10.36922/td.3670
Submitted: 15 May 2024 | Accepted: 3 July 2024 | Published: 7 August 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Osteosarcoma, a primary malignant bone tumor, is the third most common cancer in children and adolescents under 20 years old. In recent decades, many osteosarcomar elated molecular targets, including long noncoding RNA (lncRNA), have been discovered or confirmed. This study aims to elucidate the gene expression and possible regulatory mechanisms of lncRNA HA117 in osteosarcoma. To achieve this, we downloaded 51 whole-transcriptome osteosarcoma sequencing samples from the NCBI Sequence Read Archive database and performed bioinformatics analysis. Geneexpression analysis of HA117 revealed no significant difference between the tumor and adjacent tissues. However, HA117 exhibited significant down-regulation in bothfresh and formalin‐fixed paraffin-embedded (FFPE) samples after chemotherapy. Bycombining two target gene prediction methods, we identified 11 and 83 target genes for HA117 in fresh and FFPE samples, respectively. Functional analysis indicated that these target genes are mainly located in the cytoplasm and nucleus, with most related to protein binding. In addition, Reactome analysis demonstrated that these target genes participate in the regulation of multiple metabolic pathways, predominantly in cellular responses to stress. Our findings suggest that chemotherapy may regulate downstream target genes by altering the expression of HA117, thereby inducing cellular stress response. Overall, our results indicate that HA117 may not function as an oncogene but could serve as a therapeutic target in osteosarcoma.

Keywords
LncRNA
Osteosarcoma
Differentially expressed genes
Gene Ontology analysis
Reactome analysis
Target gene
Funding
This research was funded by the Natural Science Foundation of Chongqing, Project number cstc2020jcyjmsxmX0966.
Conflict of interest
The authors declare no competing interests.
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Tumor Discovery, Electronic ISSN: 2810-9775 Print ISSN: 3060-8597, Published by AccScience Publishing
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