AccScience Publishing / ITPS / Volume 7 / Issue 1 / DOI: 10.36922/itps.1343
Cite this article
81
Download
702
Views
Journal Browser
Volume | Year
Issue
Search
News and Announcements
View All
ORIGINAL RESEARCH ARTICLE

Anti-ulcer activity of aqueous ethanol extract of Rheum spiciforme and its fractions in animal model

Hafiz Muhammad Irfan1* Maham Idrees1 Kainat Jabeen1
Show Less
1 Department of Pharmacology, University of Sargodha, 40100, Sargodha, Punjab, Pakistan
INNOSC Theranostics and Pharmacological Sciences 2024, 7(1), 1343 https://doi.org/10.36922/itps.1343
Submitted: 20 July 2023 | Accepted: 29 August 2023 | Published: 13 October 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC BY-NC 4.0) ( https://creativecommons.org/licenses/by-nc/4.0/ )
Abstract

Helicobacter pylori infection, pepsin, ischemia, hypoxia, smoking, alcohol intake, hydrochloric acid, and nonsteroidal anti-inflammatory drugs are the causal factors of ulceration. In third-world countries, approximately one in two individuals has signs of gastric ulceration. Extensive literature suggested that plant-derived drugs hold the potential in the treatment of peptic ulcer, and Rheum spiciforme (family: Polygonaceae) has been known in the folk medicine for possessing medicinal effect on ulceration. To explore further along this line, the current study was designed to investigate the effects of aqueous ethanol extract of R. spiciforme roots and its fractions on peptic ulcer and to identify bioactive compounds responsible for mitigating this pathological condition. Animal model of ethanol-induced gastric ulcer was utilized in this study. The animals were pre-treated with crude plant extract, given at doses 125, 250, and 500 mg/kg, and then orally administered with butanol, aqueous, and dichloromethane fractions at doses of 250 mg/kg for 7 days. Following the treatments, a significant decrease in ulcerative lesions, ulcer index, ulcer severity score, volume, free acidity, and total acidity of gastric juice as well as a marked increase of gastric pH in the groups treated with plant extract at doses of 250 and 500 mg/kg were noted. Moreover, butanol fraction has been shown to produce effects equipotent to that of the reference drug omeprazole. Liquid chromatography-mass spectrometry analyses show that the plant extract contains emodin, aloe-emodin, and quercetin. In conclusion, this study demonstrated that the aqueous ethanol extract of R. spiciforme and its butanol fraction exhibited gastro-protective effect.

Keywords
Rheum spiciforme
Protective role
Ulcer
Ethanol
Fractions
Funding
College of Pharmacy, University of Sargodha
References
  1. Sung JJ, Kuipers EJ, El‐Serag HB, 2009, Systematic review: The global incidence and prevalence of peptic ulcer disease. Aliment Pharmacol Ther, 29: 938–946.

 

  1. Najm WI, 2011, Peptic ulcer disease. Prim Care, 38: 383– 394, vii.

 

  1. Rao SP, Amrit I, Singh V, et al., 2015, Antiulcer activity of natural compounds: A review. Res J Pharmacogn Phytochem, 7: 124.

 

  1. Yuan Y, Padol IT, Hunt RH, 2006, Peptic ulcer disease today. Nat Clin Pract Gastroenterol Hepatol, 3: 80–89.

 

  1. Ayaz M, Junaid M, Ahmed J, et al., 2014, Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L. BMC Complement Altern Med, 14: 1–9.

 

  1. Khan SW, Khatoon S, 2008, Ethnobotanical studies on some useful herbs of Haramosh and Bugrote valleys in Gilgit, northern areas of Pakistan. Pak J Bot, 40: 43.

 

  1. Tabin S, Kaur K, Singh V, et al., 2016, Meiotic and ethnobotanical studies on rheum species from Kashmir Himalaya. Cytologia, 81: 295–300.

 

  1. Boesi A, 2014, Traditional knowledge of wild food plants in a few Tibetan communities. J Ethnobiol Ethnomed, 10: 1–19.

 

  1. Sofidiya MO, Agunbiade FO, Koorbanally NA, et al., 2014, Antiulcer activity of the ethanolic extract and ethyl acetate fraction of the leaves of Markhamia tomentosa in rats. J Ethnopharmacol, 157: 1–6.

 

  1. National Research Council, 2010, Guide for the Care and Use of Laboratory Animals. Washington, DC: The National Academies Press.

 

  1. Shah NC, 1982, Herbal folk medicines in northern India. J Ethnopharmacol, 6: 293–301.

 

  1. Irfan HM, Asmawi MZ, Khan NA, et al., 2016, Effect of ethanolic extract of Moringa oleifera Lam. Leaves on body weight and hyperglycemia of diabetic rats. Pak J Nut, 15: 112–117.

 

  1. Irfan HM, Asmawi MZ, Khan NA, et al., 2017, Anti-diabetic activity-guided screening of aqueous-ethanol Moringa oleifera extracts and fractions: Identification of marker compounds. Trop J Pharm Res, 16: 543–552.

 

  1. Sahoo SK, Sahoo HB, Priyadarshini D, et al., 2016, Antiulcer activity of ethanolic extract of Salvadora indica (W.) leaves on albino rats. J Clin Diagn Res, 10: FF07.

 

  1. Al-Sayed E, El-Naga RN, 2015, Protective role of ellagitannins from Eucalyptus citriodora against ethanol-induced gastric ulcer in rats: Impact on oxidative stress, inflammation and calcitonin-gene related peptide. Phytomedicine, 22: 5–15.

 

  1. Abd El-Hady FK, El Awdan SA, Ibrahim AM, 2013, Anti-ulcerative potential of Egyptian propolis against oxidative gastric injury induced by indomethacin in rats. Asian J Med Pharm Res, 3: 35–42.

 

  1. Reddy VP, Sudheshna G, Afsar S, et al., 2012, Evaluation of anti-ulcer activity of Citrullus colocynthis fruit against pylorus ligation induced ulcers in male wistar rats. Int J Pharm Pharm Sci, 4: 446–451.

 

  1. Rasve VR, Paithankar VV, Shirsat MK, et al., 2017, Evaluation of antiulcer activity of Aconitum heterophyllum on experimental animal. World J Pharm Pharm Sci, 7: 819–839.

 

  1. Dashputre N, Naikwade N, 2011, Evaluation of anti-ulcer activity of methanolic extract of Abutilon indicum Linn leaves in experimental rats. Int J Pharm Sci Drug Res, 3: 97–100.

 

  1. Amr AR, Maysa ME, 2010, Anti-ulcer effect of cinnamon and chamomile aqueous extracts in rat models. J Am Sci, 6: 209–216.

 

  1. Vijayalakshmi M, Kiruthika R, Bharathi K, et al., 2015, Phytochemical screening by LC-MS analysis and in vitro anti-inflammatory activity of Marsilea quadrifolia plant extract. Int J Pharmtech Res, 8: 148–157.

 

  1. Abdoulrahman K, 2023, Anti-ulcer effect of Ranunculus millefoliatus on absolute alcohol-induced stomach ulceration. Saudi J Biol Sci, 30: 103711.

 

  1. Borra SK, Lagisetty RK, Mallela GR, 2011, Anti-ulcer effect of Aloe vera in non-steroidal anti-inflammatory drug induced peptic ulcers in rats. Afr J Pharm Pharmacol, 5: 1867–1871.

 

  1. Lee EB, Kim OJ, Kang SS, et al., 2005, Araloside A, an antiulcer constituent from the root bark of Aralia elata. Biol Pharm Bull, 28: 523–526.

 

  1. Ahmed O, Nedi T, Yimer EM, 2022, Evaluation of anti-gastric ulcer activity of aqueous and 80% methanol leaf extracts of Urtica simensis in rats. Metabol Open, 14: 100172.

 

  1. Lemos M, De Barros MP, Sousa JP, et al., 2007, Baccharis dracunculifolia, the main botanical source of Brazilian green propolis, displays antiulcer activity. J Pharm Pharmacol, 59: 603–608.

 

  1. Seitimova GA, Shokan AK, Tolstikova TG, et al., 2023, Antiulcer activity of anthraquinone-flavonoid complex of Rumex tianschanicus Losinsk. Molecules, 28: 2347.

 

  1. Borrelli F, Izzo AA., 2000, The plant kingdom as a source of anti‐ulcer remedies. Phytother Res, 14: 581–591.
Conflict of interest
The authors have no conflict of interest.
Share
Back to top
INNOSC Theranostics and Pharmacological Sciences, Electronic ISSN: 2705-0823 Published by AccScience Publishing