This Special Issue focuses on the genetic and molecular mechanisms that drive ovarian cancer development and progression. Ovarian cancer remains a leading cause of gynecologic cancer mortality, largely due to late diagnosis, tumor heterogeneity, and therapy resistance. This issue invites studies that investigate how genetic alterations, epigenetic changes, tumor–stroma interactions, and microenvironmental factors influence tumor initiation, growth, metastasis, and response to therapy. By integrating genomics, transcriptomics, proteomics, and spatial profiling, researchers can uncover actionable molecular signatures and identify novel therapeutic targets. The goal of this collection is to advance understanding of ovarian cancer biology, inform precision oncology strategies, and ultimately improve patient outcomes through targeted interventions.