A Novel Monoclonal Antibody PC322 Inhibit Prostate Cancer Cell Autophagy
Autophagy is an important element in tumor progression. The clinical and commercial success of monoclonal antibodies (MAbs) for cancer has emerged as the fastest growing therapy. Prostate cancer is well appropriately suited for antibody therapy. PC322 is our newly prepared prostate cancer MAbs. In our current study, we described the impacts of PC322 on autophagy and biological behaviors in vitro and explored the possible mechanism of PC322 in prostate cancer cell autophagy. Role of PC322 on autophagy of LNCaP and C4-2 cells was observed with fluorescence microscopy, western blotting, and flow cytometry. Further, the function of PC322 on biological behaviors of prostate cancer cells was detected by Cell Counting Kit-8 kit, Hoechst 33342, wound healing assay, and cell adhesion assay. Finally, we found that autophagy-related genes3 (ATG3) was upregulated with stimulation of rapamycin, while PC322 inhibited ATG3 expression. In summary, in vitro experiments demonstrated that the new MAb PC322 inhibits cell autophagy, proliferation, and metastasis of LNCaP and C4-2 cells, and promote their apoptosis. PC322 inhibits LC3 conversion and suppresses the formation of autophagosomes by reducing ATG3 expression.
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