AccScience Publishing / BH / Online First / DOI: 10.36922/bh.3996
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ORIGINAL RESEARCH ARTICLE

Human dental pulp MSCs attenuated motor neuron dysfunction and prolonged lifespan in ALS murine model

Shihe Jiang1† ,  Xiuchen Guan2† ,  Meng Shi3 ,  Ying Zhang1 ,  Xindi Li1 ,  Yingying Su3 ,  Hao Wang3 ,  Jian Zhou4 ,  Fu-Dong Shi1 ,  Songling Wang5 ,  Wei-Na Jin1*
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1 China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
2 Department of Orthodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China
3 Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
4 Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China
5 Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Capital Medical University, Beijing, China
Brain & Heart, 3996 https://doi.org/10.36922/bh.3996
Submitted: 20 June 2024 | Accepted: 4 September 2024 | Published: 10 October 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that causes skeletal muscle weakness and atrophy, resulting in respiratory failure and a short lifespan. Considering the lack of effective treatment, this study investigated the effects of human dental pulp stem cells (hDPSCs) on the clinical symptoms and potential mechanisms in a mouse model of ALS superoxide dismutase 1 (SOD1-G93A). Neurological assessments, including neurological scoring, rotarod testing, and 7-T magnetic resonance imaging, were conducted to evaluate neurological impairments. Survival rates and body weight of the mice were also recorded. Immunofluorescence staining and flow cytometry analyses were performed to investigate the number of neurons and infiltrated inflammatory cells in the spinal cord as well as the central nervous system. The results indicate that infusion of hDPSCs increased the body weight, mitigated motor neuron dysfunction, and extended the lifespan of SOD1-G93A mice by approximately 15 days. Moreover, hDPSCs infusion reduced the degree of spinal cord atrophy. Results suggested that the number of neurons in the central nervous system of SOD1-G93A mice was significantly decreased, but hDPSCs infusion resulted in an increase in these numbers. However, hDPSCs infusion had no obvious effect on microglia phenotypes in SOD1-G93A mice. This study emphasizes the potential of hDPSCs to mitigate neuronal loss in an ALS mouse model, suggesting a promising therapeutic avenue for ALS.

Keywords
Human dental pulp stem cells
Amyotrophic lateral sclerosis
Neurons
Inflammation
Murine model
Funding
This work was supported in part by the National Science Foundation of China (82122021).
Conflict of interest
Wei-Na Jin is an Editorial Board Member of this journal but was not in any way involved in the editorial and peer-review process conducted for this paper, directly or indirectly. Separately, other authors declared that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.
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Brain & Heart, Electronic ISSN: 2972-4139 Published by AccScience Publishing
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