AccScience Publishing / ARNM / Volume 2 / Issue 1 / DOI: 10.36922/arnm.2822
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ORIGINAL RESEARCH ARTICLE

Evaluation of multidrug resistance in tumors using 99mTc-doxorubicin

Bianca Gutfilen1* Sergio Augusto Lopes de Souza1 Thiago Barboza1 Bruna Fortunato Novis2 Claudia Lopes Rodrigues Chagas1 Maria Verônica Fonseca Torres de Oliveira1 Vivian M. Rumjanek2
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1 Laboratory of Labeling Cells and Molecules, Department of Radiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
2 Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Submitted: 25 January 2024 | Accepted: 28 March 2024 | Published: 3 April 2024
© 2024 by the Author (s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Doxorubicin (DOX) is a potent chemotherapeutic agent used as the first-choice drug in treating different types of cancer, such as leukemia, lymphoma, and breast cancer, among others. However, the development of multidrug resistance represents a significant obstacle to the successful treatment of tumors. In this study, we assessed whether labeled DOX could be uptaken by susceptible and multidrug-resistant cell lines and by tumors in vivo in animals. The efficiency of DOX labeling was maintained at around 90% in this work. To evaluate the effect of 99mTc-DOX on cells expressing active P-glycoprotein (Pgp), we utilized cultured human chronic myeloid leukemia K562 cells and the resistant counterpart Lucena 1 cells. Lucena 1 cell line showed overexpression of Pgp that was not observed in K562. Our results suggested that the resistance to labeled or unlabeled DOX can be reversed by Pgp inhibition and can be easily detected. In conclusion, administering 99mTc-DOX into mice enables the differentiation between multidrug-resistant tumors in a non-invasive way and the evaluation of tumor resistance and possible chemosensitizers.

Keywords
Doxorubicin
Multidrug resistance
Tc-99m
99mTc-Doxorubicin
Funding
This study was supported by grants from Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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Conflict of interest
The authors declare they have no competing interests.
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