AccScience Publishing / AJWEP / Online First / DOI: 10.36922/AJWEP025160123
REVIEW ARTICLE

Nano-biofertilizers: A promising technology for sustainable soil fertility, soil health, and environmental protection

Hayyawi W. A. Al-Juthery1 Rand A. H. G. Al-Taee2 Ali S. Alhasan1 Diaa F. Hassan3* Nisreen A. A. Al-Jassani4 Raid Shaalan Jarallah1
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1 Department of Soil and Water Resources, College of Agriculture, University of Al-Qadisiyah, Al Diwaniyah, Qadisiyyah, Iraq
2 Department of Soil and Water Resources, College of Agriculture and Forestry, University of Mosul, Mosul, Nineveh, Iraq
3 Department of Civil Engineering, College of Engineering, Al-Qasim Green University, Al-Qasim, Babylon, Iraq
4 Department of Soil and Water Resources, College of Education for Girls, University of Kufa, Kufa, Najaf, Iraq
Received: 19 April 2025 | Revised: 26 May 2025 | Accepted: 28 May 2025 | Published online: 24 June 2025
© 2025 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

The rapidly increasing global demand for food security urgently calls for agricultural technologies that can boost productivity without contributing to environmental degradation. A group of fertilizers that combines nanotechnology with beneficial microorganisms – known as nano-biofertilizers – has shown tremendous potential to enhance plant growth, increase yields, and improve soil health. Controlled nutrient release, enhanced microbial activities, and improved nutrient-use efficiency are key benefits of these new fertilizers, collectively leading to higher crop productivity with reduced environmental impact. These advantages contribute to sustainable agriculture by reducing the use of chemical fertilizers, surface soil degradation, nutrient leaching, and greenhouse gas emissions. Recent research findings have also highlighted their role in increasing plant resilience to abiotic stress factors, enhancing soil microbial diversity, and supporting ecological balance. This paper presents a comprehensive analysis of the multifaceted roles of nano-biofertilizers in modern agriculture, focusing on their contributions to plant physiology, soil health, environmental sustainability, and long-term agricultural productivity. Through empirical integration, we argue that nano-biofertilizers could be a transformative tool in advancing sustainable farming and achieving global environmental conservation goals.

Keywords
Fertilizer innovation
Microbial bioinoculant
Plant growth and yield
Soil health and sustainability
Plant-microbe interaction
Nutrient efficiency
Microbial activity
Funding
None.
Conflict of interest
The authors declare that they have no competing interests.
References
  1. Garg D, Sridhar K, Stephen Inbaraj B, Chawla P, Tripathi M, Sharma M. Nano-biofertilizer formulations for agriculture: A systematic review on recent advances and prospective applications. Bioengineering (Basel). 2023;10(9):1010. doi: 10.3390/bioengineering10091010

 

  1. Sambangi P, Gopalakrishnan S, Pebam M, Rengan AK. Nano-biofertilizers on soil health, chemistry, and microbial community: Benefits and risks. Proc Indian Natl Sci Acad. 2022;88(3):357-368. doi: 10.1007/s43538-022-00094-1

 

  1. Miranda-Villagómez E, Trejo-Téllez LI, Gómez-Merino FC, Sandoval-Villa M, Sánchez-García P, Aguilar-Méndez MÁ. Nanophosphorus fertilizer stimulates growth and photosynthetic activity and improves P status in rice. J Nanomater. 2019;2019(1):5368027. doi: 10.1155/2019/5368027

 

  1. Madlala NC, Khanyile N. Examining the correlation between the inorganic nano-fertilizer physical properties and their impact on crop performance and nutrient uptake efficiency. Nanomaterials (Basel). 2024;14(8):1263. doi: 10.3390/nano14151263

 

  1. Liu R, Lal R. Nano-enhanced fertilizers for improved crop productivity and soil sustainability. Crit Rev Plant Sci. 2021;40(1):1-24.

 

  1. Badiri ZK, Al-Juthery HW. Effect of spraying some bio and nano stimulants fortified with potassium on some qualitative traits and active substances in rice grain. AIP Conf Proc. 2024;3079(1):020013. doi: 10.1063/5.0202277

 

  1. Mishra S, Tripathi DK, Singh VP. Microbial synergy with nano-biofertilizers in sustainable agriculture. Plant Soil Sci Rev. 2020;52(1):89-104.

 

  1. Singh S, Prasad R. Nano-biofertilizers: A sustainable approach for soil fertility. Agric Adv. 2020;9(2):178-198.

 

  1. Kumar R, Ghosh D, Sharma S. Microbial interactions in nano-biofertilizer treated soils. Soil Biol Rep. 2021;89:219-232.

 

  1. Subramanian KS, Rahale CS. Nano-encapsulated nitrogen fertilizers for sustainable crop production. J Agric Sci. 2015;42(1):45-58.

 

  1. Parikh SJ, Mukherjee S. Advances in nano-fertilizers for soil nutrient management. Annu Rev Plant Biol. 2020;71(1):405-432.

 

  1. Mukherjee S, Singh R. Advances in nano-biofertilizers for enhanced soil microbial activity. Microbiol Today. 2021;28(2):212-226.

 

  1. Mishra S, Tripathi DK, Singh VP. Synergistic effects of nanoparticles and biofertilizers. Environ Sci Biotechnol. 2017;31(4):405-417.

 

  1. Liu X, Feng Z, Chen L. Nano-fertilizers and their potential in precision agriculture. Adv Soil Res. 2019;35(2):198-213.

 

  1. Ditta A, Arshad M, Ibrahim M. Zinc oxide nano-biofertilizers and their effect on soil fertility. J Agron Soil Sci. 2018;65(3):423-437.

 

  1. Raliya R, Tarafdar JC, Biswas P. Nano-P biofertilizers for sustainable agriculture. Soil Biol Rep. 2013;52(2):119-132.

 

  1. Shang J, Wang D, Xiang H. Impact of nano-silica biofertilizers on soil organic matter and microbial activity. Environ Sci J. 2014;45(2):112-125.

 

  1. Singh B, Choudhary R, Verma N. Nano-biofertilizers and their role in mitigating climate change impacts. Clim Resil Agric J. 2022;17(3):112-130.

 

  1. Verma A, Srivastava R, Gupta R. Carbon sequestration and soil sustainability with nano-fertilizers. Soil Environ Res. 2022;12(3):98-114.

 

  1. Chen H, Liu L, Zhang Z. Advances in Nano-Biofertilizers for Sustainable Agriculture. Vol. 12. London: Springer Nature; 2020. p. 45-60.

 

  1. Al-Juthery HW, Lahmoud NR, Alhasan AS, Al-Jassani NA, Houria A. Nano-fertilizers as a novel technique for maximum yield in wheat biofortification (article review). IOP Conf Ser Earth Environ Sci. 2022;1060(1):012043. doi: 10.1088/1755-1315/1060/1/012043

 

  1. Rai S, Verma A, Choudhary P. Nano-biofertilizers and their role in sustainable farming. J Nano Agric. 2021;10(2):55-72.

 

  1. Drebee HA, Abdul Razak NA, Mohsen AA. Maize production forecasting in Iraq: A Box-Jenkins approach for the period of 2022-2026. IOP Conf Ser Earth Environ Sci. 2023;1259(1):012128. doi: 10.1088/1755-1315/1259/1/012128

 

  1. Sharma T, Singh B. Advancing soil fertility using nano-biofertilizers. Nat Rev Soil Sci. 2022;5(4):301-320.

 

  1. Hussain S, Ahmad N, Khan R. Impact of nano-biofertilizers on enzymatic activities in soil. Soil Biol Biochem. 2021;32(1):88-101.

 

  1. Jafaar AA, Mohammed RJ, Hassan DF. Effect of phosphorus fertilizer and irrigation level on desert soil management and potato yield. Int J Agric Stat Sci. 2022;18(2):1-11.

 

  1. Patel M, Joshi R, Shah S. Enhancing soil health through bio-nanotechnology. Sustain Agric Rev. 2020;15(1):210-225.

 

  1. Hamid MQ. Mycorrhiza and Trichoderma fungi role in improving soil physical properties planted with maize (Zea mays L.). SABRAO J Breed Genet. 2025;57(1):260-269. doi: 10.54910/sabrao2025.57.1.25

 

  1. Kumar V, Yadav P, Thakur R. Microbial interactions in soil: Role of nano-biofertilizers. Agric Microbiol J. 2023;29(3):120-135.

 

  1. Ghosh S, Mukherjee A. Effect of nano-biofertilizers on soil microbiota and plant health. J Agric Sci. 2021;18(4):233-245.

 

  1. Al-Juthery HW, Yousif SAA, Lahmod NR, Alhasan AS, Tiamooz SH, Musa RF. Response wheat to spray some of synthetic nano fertilizers. IOP Conf Ser Earth Environ Sci. 2022;1060:012030.

 

  1. Singh R, Kumar P, Gupta A. Soil organic matter improvement using nano-biofertilizers. Int J Soil Sci. 2021;14(3):189-203.

 

  1. Hundi HK, Hamid MQ, Noori AA. Role of ochrobactrum bacteria and organic matter in plant growth and the content of N, P, and K under soil salinity stress. J Environ Earth Sci. 2025;7(5):130-139. doi: 10.30564/jees.v7i5.8777

 

  1. Singh J, Rathore A, Gupta R. Nano-biofertilizers: An eco-friendly solution for sustainable agriculture. Int J Agric Innov. 2020;12(3):178-195.

 

  1. Rai M, Ribeiro C, Mattoso L, Duran N. Nanotechnology applications in agriculture: An emerging trend. Nanosci Agric. 2021;14(5):269-288.

 

  1. Akol AM, Nassif N, Jaddoa KA, Radhi K, Hassan DF. Effect of irrigation methods, tillage systems and seeding rate on water consumption, biological yield and harvest index of wheat (Triticum aestivum L.). Int J Agric Stat Sci. 2021;17(3):230-242.

 

  1. Sharma R, Kumar S, Bhardwaj D. Nanoparticles and their role in plant growth promotion. J Plant Physiol Growth. 2019;11(4):299-314.

 

  1. Kumar P, Sharma S, Singh N. Biofertilizers and their role in sustainable agriculture. Curr Adv Agric Sci. 2020;8(4):311-328.

 

  1. Akol AM, Hassan DF, Mohammed RJ, et al. Optimizing wheat yield and water use efficiency using AquaCrop model calibration and validation in various irrigation and tillage systems under climate change. Soil Sci Ann. 2024;75(3):1-12. doi: 10.37501/soilsa/195823

 

  1. Mukherjee A, Ghosh A, Saha B. Environmental benefits of nano-biofertilizers in modern agriculture. Agric Sci Technol Rev. 2022;20(1):85-102.

 

  1. Drebee HA, Razak NAA, Shaybth RT. Understanding the causes of the decline in the iraqi agricultural sector’s contribution to the GDP. IOP Conf Ser Earth Environ Sci. 2022;1060(1):012146. doi: 10.1088/1755-1315/1060/1/012146

 

  1. Patra JK, Mishra RR, Thatoi H. Microbial biofertilizers and their role in soil fertility management. Microb Biotechnol Adv. 2020;6(2):198-210.

 

  1. Salman EE, Akol AM, Abdel Hamza JS, Naje AS. Implementation of the AquaCrop model for forecasting the effects of climate change on water consumption and potato yield under various irrigation techniques. Nat Environ Pollut Technol. 2024;23(2):1201-1207. doi: 10.46488/NEPT.2024.v23i02.056

 

  1. Meena H, Prasad R, Yadav S. Application of nano-biofertilizers in wheat for enhancing productivity. J Crop Improv. 2020;15(2):145-167.

 

  1. Rajput VD, Minkina T, Fedorenko A. Nano-fertilizers for sustainable agriculture: An overview of recent developments. J Agric Nanotechnol. 2021;7(1):33-57. doi: 10.3390/nano12010173

 

  1. Hassan DF, Ati AS, Neima AS. Calibration and evaluation of aquacrop for maize (Zea mays L.) under different irrigation and cultivation methods. J Ecol Eng. 2021;22(10):192-204.

 

  1. Karunakaran A, Fathima Y, Singh P, Beniwal R, Singh J, Ramakrishna W. Next-generation biofertilizers: nanoparticle-coated plant growth-promoting bacteria biofertilizers for enhancing nutrient uptake and wheat growth. Agriculture. 2024;14(4):517. doi: 10.3390/agriculture14040517

 

  1. Singh RR, Saurabh S, Prakash V, et al. Enhancing sustainability in agriculture with nanofertilizers. SN Appl Sci. 2024;6(1):267. doi: 10.1007/s42452-024-06267-5

 

  1. González M, Jodeh S, Alkowni R. Nano bio fertilizer capsules for sustainable agriculture. Sci Rep. 2024;14:62973. doi: 10.1038/s41598-024-62973-5

 

  1. Maaz TM, Dobermann A, Lyons SE, Thomson AM. Review of research and innovation on novel fertilizers for crop nutrition. NPJ Sustain Agric. 2025;3:25. doi: 10.1038/s44264-025-00066-0

 

  1. Liu R, Lal R. Synthetic apatite nanoparticles as a phosphorus fertilizer for soybean (Glycine max). Sci Rep. 2014;4(1):5686. doi: 10.1038/srep05686

 

  1. Yadav AN, Kour D, Yadav N. Nano-biofertilizers for agricultural sustainability. J Appl Biol Biotechnol. 2023;11(5):1-4. doi: 10.7324/JABB.2023.16266

 

  1. Patel C, Singh J, Karunakaran A, Ramakrishna W. Evolution of nano-biofertilizer as a green technology for agriculture. Agriculture. 2023;13(10):1865. doi: 10.3390/agriculture13101865

 

  1. Verma R, Pathak D, Rajput VD. Nanocarrier-based encapsulated fertilizers: Recent advances and field application prospects. Environ Technol Innov. 2023;30:103093. doi: 10.1016/j.eti.2022.103093

 

  1. Isabel JB, Balamurugan A, Devi PR, Periyasamy S. Chitosan-encapsulated microbial biofertilizer: A breakthrough for enhanced tomato crop productivity. Int J Biol Macromol. 2024;260:129462. doi: 10.1016/j.ijbiomac.2024.12946

 

  1. Ingle PU, Shende SS, Shingote PR, et al. Chitosan nanoparticles (ChNPs): A versatile growth promoter in modern agricultural production. Heliyon. 2022;8(11):e11893. doi: 10.1016/j.heliyon.2022.e11893

 

  1. Psathas P, Zindrou A, Papachristodoulou C, Boukos N, Deligiannakis Y. Tandem control of la-doping and CuO-heterojunction on SrTiO3 perovskite by double-nozzle flame spray pyrolysis: Selective H2 vs. CH4 photocatalytic production from H2O/CH3OH. Nanomaterials. 2023;13(3):482. doi: 10.3390/nano13030482

 

  1. Kottegoda N, Sandaruwan C, Priyadarshana G, et al. Urea-hydroxyapatite nanohybrids for slow release of nitrogen. ACS Nano. 2017;11(2):1214-1221. doi: 10.1021/acsnano.6b07781

 

  1. Yin N, Yang P, Liu S, Pan S, Zhang Z. AI for tribology: Present and future. Friction. 2024;12(6):1060-1097. doi: 10.1007/s40544-024-0879-2

 

  1. Kalhapure R, Patil A. Economic feasibility of nano-biofertilizer production: A comparative assessment of synthesis methods. Curr Res Environ Sustain. 2022;4:100147. doi: 10.1016/j.crsust.2022.100147

 

  1. Drebee HA, Razak NAA, Brisam AA. What are the determinants of investment in the Iraqi agricultural sector? IOP Conf Ser Earth Environ Sci. 2021;735(1):012038. doi: 10.1088/1755-1315/735/1/012038

 

  1. Hamid MQ. Response of physical properties of sandy soil treated with different levels of natural soil conditioners zeolite and perlite. Sarhad J Agric. 2025;41(2):591-599. doi: 10.17582/journal.sja/2025/41.2.591.599

 

  1. Ghormade V, Deshpande MV, Paknikar KM. Perspectives for nano-biotechnology enabled protection and nutrition of plants. Biotechnol Adv. 2011;29(6):792-803. doi: 10.1016/j.biotechadv.2011.06.007

 

  1. Jatav RS, Singh N, Tiwari P. Nano-biofertilizers for sustainable agriculture and soil resilience. Front Plant Sci. 2023;14:112-134.

 

  1. Lateef A, Nazir R, Javed S. Silica nanoparticles as plant growth enhancers. Plant Biotechnol Rep. 2021;15(4):456-472.

 

  1. Adisa IO, Reddy DHK, Arslan Z. Nanotechnology in fertilizer development: Current trends and future perspectives. Environ Sci Pollut Res. 2020;27(11):12459-12476.

 

  1. Shang Y, Yang S, Wang Y. Nanocarriers for controlled fertilizer release: Advances and applications. Adv Mater Sustain Agric. 2021;39(1):25-38.

 

  1. Romanovski V, Zhang Z, Akbarisehat A. Nanobiosensors for precision farming and sustainable agriculture. In: Agricultural Sustainability through Nanotechnology. USA: CRC Press; 2025. p. 156-174.

 

  1. Upadhyay PK, Dey A, Singh VK, et al. Conjoint application of nano-urea with conventional fertilizers: An energy efficient and environmentally robust approach for sustainable crop production. Plos one. 2023;18(7):e0284009. doi: 10.1371/journal.pone.0284009

 

  1. Atanda SA, Shaibu RO, Agunbiade FO. Nanoparticles in agriculture: Balancing food security and environmental sustainability. Discov Agric. 2025;3(1):26. doi: 10.1007/s44279-025-00159-x

 

  1. Arora PK, Tripathi S, Omar RA, et al. Next-generation fertilizers: The impact of bionanofertilizers on sustainable agriculture. Microb Cell Fact. 2024;23:254. doi: 10.1186/s12934-024-02528-5

 

  1. Upadhyay PK, Dey A, Singh VK, et al. Changes in microbial community structure and yield responses with the use of nano-fertilizers of nitrogen and zinc in wheat-maize system. Sci Rep. 2024;14:1100. doi: 10.1038/s41598-023-48951-3

 

  1. Chen S, Teng Y, Luo Y, Kuramae E, Ren W. Threats to the soil microbiome from nanomaterials: A global meta and machine-learning analysis. Soil Biol Biochem. 2024;188:109248. doi: 10.1016/j.soilbio.2023.109248

 

  1. Rai M, Acharya R, Singh P. Nanotechnology-based biofertilizers for sustainable agriculture. J Nanosci Nanotechnol. 2022;22(6):4512-4524.

 

  1. Usman K, Ahmed A, Alam M. Precision agriculture and nanotechnology: The future of farming. Precision Agric Rev. 2023;28(5):189-205.

 

  1. Sarkar A, Rakshit A. Bio-nanocomposites in sustainable agriculture. Environ Sci Adv. 2020;4(2):56-72.

 

  1. Bhagat S, Kumar R, Sharma S. Sustainable agriculture through nano-biofertilizers: A review. J Agric Environ Res. 2021;45(2):78-89.

 

  1. Khan S, Malik A, Hussain M. Role of nanotechnology in plant stress tolerance: An overview. J Plant Sci Technol. 2021;12(4):234-248.

 

  1. Mishra V, Tripathi RD, Srivastava S. Environmental and safety concerns of nano-fertilizers: A critical review. Environ Nanotechnol Monit Manage. 2023;18(3):100481.

 

  1. Verma R, Gupta S, Mehta D. Policy and regulatory challenges in nano-agriculture. J Agric Policy Sustain. 2022;13(1):45-63.

 

  1. Sakhno Y, Miletto I, Paul G, Jaisi DP. A novel route to enhance the dissolution of apatite: Structural incorporation of hydrogen phosphate. NanoImpact. 2022;28:100422. doi: 10.1016/j.impact.2022.100422

 

  1. Singh D, Gurjar BR. Nanotechnology for agricultural applications: Facts, issues, knowledge gaps, and challenges in environmental risk assessment. J Environ Manag. 2022;322:116033. doi: 10.1016/j.jenvman.2022.116033

 

  1. Noguera-Oviedo K, Aga DS. Nanofertilizers and U.S. Regulations: What we know and what we don’t. Nano Today. 2020;35:100950. doi: 10.1016/j.nantod.2020.100950

 

  1. Wang K, Yin D, Sun Z, Wang Z, You S. Distribution, horizontal transfer and influencing factors of antibiotic resistance genes and antimicrobial mechanism of compost tea. J Hazard Mater. 2022;438:129395. doi: 10.1016/j.jhazmat.2022.129395

 

  1. Sharma P, Pal N, Kumawat M, et al. Antibiotic and non-antibiotic determinants of antimicrobial resistance: Insights from water ecosystems. ACS EST Water. 2024;4(11):4671-4689. doi: 10.1021/acsestwater.4c00305

 

  1. Howe JA, McDonald MD, Burke J, et al. Influence of fertilizer and manure inputs on soil health: A review. Soil Security. 2024;16(3):100155. doi: 10.1016/j.soisec.2024.100155

 

  1. Pahalvi HN, Rafiya L, Rashid S, Nisar B, Kamili AN. Chemical fertilizers and their impact on soil health. In: Dar GH, Bhat RA, Mehmood MA, Hakeem KR, editors. Microbiota and Biofertilizers. Vol. 2. Cham: Springer; 2021. doi: 10.1007/978-3-030-61010-4_1

 

  1. Kumar Meena A, Rupesh T, Siddartha Naik B, et al. Impact of nitrogen fertilizers on soil health. Int J Curr Microbiol Appl Sci. 2020;11:993-1008.

 

  1. Hassan DF, Ati AS, Naima AS. Evaluation of the performance of the aquacrop model under different irrigation and cultivation methods and their effect on water consumption. Iraqi J Agric Sci. 2023;54(2):478-490.

 

  1. Mohammed RJ, Suliman AA. Land suitability assessment for wheat production using analytical hierarchy process and parametric method in babylon province. J Ecol Eng. 2023;24(7):75-87.
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