AccScience Publishing / ITPS / Volume 6 / Issue 1 / DOI: 10.36922/itps.273
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RESEARCH ARTICLE

Orally Administered Aqueous Extract of Pleurotus ostreatus Ameliorates Hyperglycemia in Streptozotocin-Induced Diabetic Rats

Pratima Vishwakarma1* Nijendra Nath Tripathi1 Veena B. Kushwaha2 Siddhartha Kumar Mishra3*
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1 Bacteriology and Natural Pesticide Laboratory, Department of Botany, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India
2 Department of Zoology, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India
3 Department of Biochemistry, University of Lucknow, Lucknow, India
INNOSC Theranostics and Pharmacological Sciences 2023, 6(1), 1–14; https://doi.org/10.36922/itps.273
Submitted: 22 November 2022 | Accepted: 15 March 2023 | Published: 28 March 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC BY-NC 4.0) ( https://creativecommons.org/licenses/by-nc/4.0/ )
Abstract

Pleurotus ostreatus (Jacq.) P. Kumm. (Family: Pleurotaceae), commonly known as oyster mushroom, has been widely used to treat various ailments from simple headache to serious ones like diabetes. The mushroom has been shown to exert anti-inflammatory and antioxidant activities. In the present study, aqueous extract of P. ostreatus (AEPO) was examined for its antidiabetic activity in streptozotocin (STZ)-induced diabetic rats. STZ was administered to rats at 50 mg/kg body weight (i.p.), AEPO was orally administered at 100 and 200 mg/kg body weight, and metformin (500 mg/kg) was administered as positive control. The hypoglycemic effects of the AEPO were analyzed by assessing the blood glucose levels (oral glucose tolerance test, acute and postprandial antihyperglycemic activity), lipid parameters, and other hematological studies in comparison to standard drug (metformin). Results showed that AEPO caused a 26% reduction in blood glucose during fasting while 45% reduction in blood glucose during postprandial antihyperglycemic test in STZ-diabetic rats. It also helped in the normalizations of various complications associated with diabetes mellitus in rats. Observations from the current experiments indicate that P. ostreatus help in reduction of blood glucose level, thus confirming its antidiabetic activity in STZ-induced diabetes in rats. This study further advocates that supplementation of edible mushroom P. ostreatus could be a preventive approach in diabetes as well as in obesity management.

Keywords
Antidiabetic
Pleurotus ostreatus
Streptozotocin
Metformin
Funding
None.
References
[1]

Wadkar, K.A.; Magdum, C.S.; Patil, S.S.; Naikwade, N.S. Anti- Diabetic Potential and Indian Medicinal Plants. J. Herb. Med. Toxicol., 2008, 2, 45–50.

[2]

Oluba, O.M.; Onyeneke, E.C.; Ojieh, G.C.; Idonije, B.O. Evaluation of the Hypoglycemic Effect of Aqueous Extract of Ganoderma Lucidum on STZ-Induced Diabetic Wistar Rats. Ann. Biol. Res., 2010, 1, 41–9.

[3]

Van Dyke, M.E.; Crawford, N.D.; Lewis, T.T. Van Dyke et al. Respond to Methodological Considerations in Investigating Discrimination. Am. J. Epidemiol., 2022, 191, 384–5.

[4]

Meares, G.P.; Fontanilla, D.; Broniowska, K.A.; Andreone, T.; Lancaster, J.R. Jr.; Corbett, J.A. Differential Responses of Pancreatic β-Cells to ROS and RNS. Am. J. Physiol. Endocrinol. Metab., 2013, 304, E614–22.

[5]

Adam, Z.; Ismail, A.; Khamis, S.;Mokhtar, M.H.; Hamid, M. Antihyperglycemic Activity of F. Deltoidea Ethanolic Extract in Normal Rats. Sains. Malays., 2011, 40, 489–95.

[6]

Pullaiah, T.; Naidu, K.C. Antidiabetic Plants in India and Herbal Based Antidiabetic Research. Regency Publications, New Delhi, 2003. p1–10. 

[7]

Rushita, S.; Vijayakumar, M.; Noorlidah, A.; Abdulla, M.A.; Vikineswary, S. Effect of Pleurotus Citrinopileatus on Blood Glucose, Insulin and Catalase of Streptozotocin-Induced Type 2 Diabetes Mellitus Rats. J. Anim. Plant Sci., 2013, 23, 1566–71.

[8]

Alam, N.; Amin, R.; Khan, A.; Ara, I.; Shim, M.J.; Lee, M.W.; Lee, T.S. Nutritional Analysis of Cultivated Mushrooms in Bangladesh-Pleurotus Ostreatus, Pleurotus Sajor-Caju, Pleurotus Florida and Calocybe Indica. Mycobiology, 2008, 36, 228–32.

[9]

Jin, Z.; Li, Y.; Ren, J.; Qin, N. Yield, Nutritional Content, and Antioxidant Activity of Pleurotus Ostreatus on Corncobs Supplemented with Herb Residues. Mycobiology, 2018, 46, 24–32.

[10]

Gąsecka, M.; Mleczek, M.; Siwulski, M.; Niedzielski, P. Phenolic Composition and Antioxidant Properties of Pleurotus Ostreatus and Pleurotus Eryngii Enriched with Selenium and Zinc. Eur. Food Res. Technol., 2016, 242, 723–32. 

[11]

Ito, M.; Kondo, Y.; Nakatani, A.; Hayashi, K.; Naruse, A. Characterization of Low Dose Streptozotocin-Induced Progressive Diabetes in Mice. Environ. Toxicol. Pharmacol., 2001, 9, 71–8.

[12]

Atri, N.S.; Sharma, S.K.; Joshi, R.; Gulati, A.; Gulati, A. Amino Acid Composition of Five Wild Pleurotus Species Chosen from North West India. Eur. J. Biol. Sci., 2012, 4, 31–4.

[13]

Gallagher, A.; Flatt, P.; Duffy, G.; Abdel-Wahab, Y.H. The Effects of Traditional Antidiabetic Plants on in Vitro Glucose Diffusion. Nutr. Res., 2003, 23, 413–24.

[14]

Kim, D.H.; Yang, B.K.; Jeong, S.C.; Park, J.B.; Cho, S.P.; Das, S.; Yun, J.W.; Song, C.H. Production of a Hypoglycemic, Extracellular Polysaccharide from the Submerged Culture of the Mushroom, Phellinus Linteus. Biotechnol. Lett., 2001, 23, 513–7.

[15]

Jayasuriya, W.J.; Handunnetti, S.M.; Wanigatunge, C.A.; Fernando, G.H.; Abeytunga, D.T.; Suresh, T.S. Anti-Inflammatory Activity of Pleurotus Ostreatus, a Culinary Medicinal Mushroom, in Wistar Rats. Evid. Based Comp. Alternat. Med., 2020, 2020, 6845383.

[16]

Dkhil, M.A.; Diab, M.S.; Lokman, M.S.; El-Sayed, H.; Bauom, A.A.; Al-Shaebi, E.M.; Al-Quraishy, S. Nephroprotective Effect of Pleurotus Ostreatus Extract Against Cadmium Chloride Toxicity in Rats. An. Acad. Bras. Ciênc., 2020, 92, e20191121.

[17]

Jayakumar, T.; Ramesh, E.; Geraldine, P. Antioxidant Activity of the Oyster Mushroom, Pleurotus Ostreatus, on CCl(4)-Induced Liver Injury in Rats. Food Chem. Toxicol., 2006, 44, 1989–96.

[18]

Ishfaq, P.M.; Mishra, A.; Mishra, S.; Mishra, S.K.; Ahmad, Z.; Gayen, S.; Subodh, K.; Tripathi, S. Inonotus Obliquus Aqueous Extract Suppresses Carbon Tetrachloride-Induced Hepatic Injury through Modulation of Antioxidant Enzyme System and Anti- Inflammatory Mechanism. Clin. Cancer Drugs, 2021, 8, 122–36.

[19]

Ishfaq, P.M.; Mishra, S.; Mishra, A.; Mishra, S.K.; Ahmad, Z.; Gayen, S.; Jain, S.K.; Tripathi, S. Inonotus Obliquus Aqueous Extract Prevents Histopathological Alterations in Liver Induced by Environmental Toxicant Microcystin. Curr. Res. Pharmacol. Drug Discov., 2022, 3, 100118.

[20]

Kang, J.H.; Jang, J.E.; Mishra, S.K.; Lee, H.J.; Nho, C.W.; Shin, D.; Jin, M.; Kim, M.K.; Choi, C.; Oh, S.H. Ergosterol Peroxide from Chaga Mushroom (Inonotus Obliquus) Exhibits Anti-Cancer Activity by Down-Regulation of the β-Catenin Pathway in Colorectal Cancer. J. Ethnopharmacol., 2015, 173, 303–12.

[21]

Mishra, S.K.; Kang, J.H.; Kim, D.K.; Oh, S.H.; Kim, M.K. Orally Administered Aqueous Extract of Inonotus Obliquus Ameliorates Acute Inflammation in Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice. J. Ethnopharmacol., 2012, 143,524–32.

[22]

Mishra, S.K.; Kang, J.H.; Song, K.H.; Park, M.S.; Kim, D.K.; Park, Y.J.; Chop, C.; Kim, H.M.; Kim, M.K.; Oh, S.H. Inonotus Obliquus Suppresses Proliferation of Colorectal Cancer Cells and Tumor Growth in Mice Models by Downregulation of β-Catenin/ NF-κB-Signaling Pathways. Eur. J. Inflamm., 2013, 11, 615–29.

[23]

American Diabetes Association. Postprandial Blood Glucose. American Diabetes Association. Diabetes Care, 2001, 24, 775–8.

[24]

Ernsberger, P. The Glucose Tolerance Test as a Laboratory Tool with Clinical Implications. In: Glucose Tolerance. InTech, London, United Kingdom, 2012. p1–10.

[25]

Milosevic, D.; Panin, V.L. Relationship Between Hematological Parameters and Glycemic Control in Type 2 Diabetes Mellitus Patients. J. Med. Biochem., 2019, 38, 164–71.

[26]

Galicia-Garcia, U.; Benito-Vicente, A.; Jebari, S.; Larrea-Sebal, A.; Siddiqi, H.; Uribe, K.B.; Ostolaza, H.; Martin, C. Pathophysiology of Type 2 Diabetes Mellitus. Int. J. Mol. Sci., 2020, 21, 6275.

[27]

Rahimah, S.B.; Djunaedi, D.D.; Soeroto, A.Y.; Bisri, T. The Phytochemical Screening, Total Phenolic Contents and Antioxidant Activities in Vitro of White Oyster Mushroom (Pleurotus Ostreatus) Preparations. Open Access Maced J. Med. Sci., 2019, 7, 2404–12.

[28]

Anitha, M.; Sakthidevi, G.; Muthukumarasamy, S.; Mohan, V.R. Effect of Cynoglossum zeylanicum (Vehl ex Hornem) Thunb. Ex Lehm on Oral Glucose Tolerance in Rats. J. Appl. Pharm. Sci., 2012, 2, 75–8. 

[29]

Badole, S.L.; Bodhankar, S.L. Interaction Study of Aqueous Extract of Pleurotus Pulmonarius (Fr.) Quel.-Champ with Acarbose in Alloxan Induced Diabetic Mice. J. Appl. Biomed., 2007, 5, 157–66. 

[30]

Cha, W.S.; Ding, J.L.; Shin, H.J.; Kim, J.S.; Kim, Y.S.; Choi, D.; Lee, C.W. Effect of Fomitopsis Pinicola Extract on Blood Glucose and Lipid Metabolism in Diabetic Rats. Korean J. Chem. Eng., 2009, 26, 1696–9.

[31]

Agrawal, R.P.; Chopra, A.; Lavekar, G.S.; Padhi, M.M.; Srikanth, N.; Ota, S.; Jain, S. Effect of Oyster Mushroom on Glycemia, Lipid Profile and Quality of Life in Type 2 Diabetic patients. Aust. J. Med. Herbal., 2010, 22, 50–4.

[32]

Hwang, H.J.; Kim, S.W.; Baek, Y.M.; Lee, S.H.; Hwang, H.S.; Kumar, S.G.; Rahman, M.A.; Yun, J.W. Differential Expression of Liver Proteins in Streptozotocin-Induced Diabetic Rats in Response to Hypoglycemic Mushroom Polysaccharides. Korean J. Chem. Eng., 2008, 25, 308–22.

[33]

Jeong, S.C.; Jeong, Y.T.; Yang, B.K.; Islam, R.; Koyyalamudi, S.R.; Pang, G.; Cho, K.Y.; Song, C.H. White Button Mushroom (Agaricus Bisporus) Lowers Blood Glucose and Cholesterol Levels in Diabetic and Hypercholesterolemic Rats. Nutr. Res., 2010, 30, 49–56.

[34]

Li, T.H.; Hou, C.C.; Chang, C.L.; Yang, W.C. Anti- Hyperglycemic Properties of Crude Extract and Triterpenes from Poria Cocos. Evid. Based Complement. Alternat. Med., 2011, 2011, 128402. 

[35]

Rajeswari, P.; Krishnakumari, S. Potent Antihyperglycaemic Activity of Calocybe Indica in Streptozotocin Induced Diabetic Rats Antihyperglycemic Activity of Calocybe Indica. Int. J. Pharm. Sci., 2013, 5, 512–5.

[36]

Yang, U.K.; Jeong, S.C.; Lee, H.J.; Sohn, D.H.; Song, C.H. Antidiabetic and Hypolipidemic Effects of Collybia Confluens Mycelia Produced by Submerged Culture in Streptozotocin- Diabetic Rats. Arch. Pharm. Res., 2006, 29, 73–9.

Conflict of interest
The authors declare no conflict of interest related to this publication.
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INNOSC Theranostics and Pharmacological Sciences, Electronic ISSN: 2705-0823 Published by AccScience Publishing