AccScience Publishing / IJB / Volume 5 / Issue 1 / DOI: 10.18063/ijb.v5i2.210
Submit to IJB
Cite this article
16
Download
663
Views
Journal Browser
Volume | Year
Issue
Search
Forthcoming Issue
Current Issue
View All
News and Announcements
View All
RESEARCH ARTICLE

Application of piezoelectric cells printing on three-dimensional porous bioceramic scaffold for bone regeneration

Ming-You Shie1,2,3† ,  Hsin-Yuan Fang4† ,  Yen-Hong Lin2,5 ,  Alvin Kai-Xing Lee2,4 ,  Joyce Yu2,4 ,  Yi-Wen Chen6,7*
Show Less
1 School of Dentistry, China Medical University, Taichung City, Taiwan
2 Three-dimensional Printing Medical Research Center, China Medical University Hospital, Taichung, Taiwan
3 Department of Bioinformatics and Medical Engineering, Asia University, Taichung City, Taiwan
4 School of Medicine, China Medical University, Taichung City, Taiwan
5 5 The Ph.D. Program for Medical Engineering and Rehabilitation Science, China Medical University, Taichung, Taiwan
6 Graduate Institute of Biomedical Sciences, China Medical University, Taichung City, Taiwan
7 Three-dimensional Printing Medical Research Institute, Asia University, Taichung City, Taiwan
IJB 2019, 5(1), 210 https://doi.org/10.18063/ijb.v5i2.210
(This article belongs to the Special Issue Bioprinting in Asia)
© Invalid date by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Download PDF
Cite
XML
Abstract

In recent years, the additive manufacture was popularly used in tissue engineering, as the various technologies for this field of research can be used. The most common method is extrusion, which is commonly used in many bioprinting applications, such as skin. In this study, we combined the two printing techniques; first, we use the extrusion technology to form the ceramic scaffold. Then, the stem cells were printed directly on the surface of the ceramic scaffold through a piezoelectric nozzle. We also evaluated the effects of polydopamine (PDA)-coated ceramic scaffolds for cell attachment after printing on the surface of the scaffold. In addition, we used fluorescein isothiocyanate to simulate the cell adhered on the scaffold surface after ejected by a piezoelectric nozzle. Finally, the attachment, growth, and differentiation behaviors of stem cell after printing on calcium silicate/polycaprolactone (CS/PCL) and PDACS/PCL surfaces were also evaluated. The PDACS/PCL scaffold is more hydrophilic than the original CS/PCL scaffold that provided for better cellular adhesion and proliferation. Moreover, the cell printing technology using the piezoelectric nozzle, the different cells can be accurately printed on the surface of the scaffold that provided and analyzed more information of the interaction between different cells on the material. We believe that this method may serve as a useful and effective approach for the regeneration of defective complex hard tissues in deep bone structures.

Keywords
Piezoelectric printing
Polydopamine
Bone tissue engineering
Calcium silicate
Polycaprolactone
Drop-on-demand
References

1. Placone JK, Engler AJ, 2018, Recent Advances in Extrusion-based 3D Printing for Biomedical Applications. Adv Healthc Mater, 7:1701161. DOI 10.1002/adhm.201701161. 
2. Chiu YC, Fang HY, Hsu TT, et al., 2017, The Characteristics of Mineral Trioxide Aggregate/Polycaprolactone 3-dimensional Scaffold with Osteogenesis Properties for Tissue Regeneration. J Endod, 43:923-9. DOI 10.1016/j. joen.2017.01.009. 
3. Sultana A, Ghosh SK, Sencadas V, et al., 2017, Human Skin Interactive Self-powered Wearable Piezoelectric Bio-e-skin by Electrospun Poly-l-lactic Acid Nanofibers for Non-invasive Physiological Signal Monitoring. J Mater Chem B, 5:7352-9. DOI 10.1039/c7tb01439b. 
4. Wu Y, Wong YS, Fuh YHJ, 2017, Degradation Behaviors of Geometric Cues and Mechanical Properties in a 3D Scaffold for Tendon Repair. J Biomed Mater Res Part A, 105:1138-49. DOI 10.1002/jbm.a.35966. 
5. Vijayavenkataraman S, Zhang S, Thaharah S, et al., 2018, Electrohydrodynamic Jet 3D Printed Nerve Guide Conduits (NGCs) for Peripheral Nerve Injury Repair. Polymers, 10:753. DOI 10.3390/polym10070753. 
6. Choi M, Heo J, Yang M, et al., 2017, Inkjet Printing-based Patchable Multilayered Biomolecule-containing Nanofilms for Biomedical Applications. ACS Biomater Sci Eng, 3:870- 4. DOI 10.1021/acsbiomaterials.7b00138. 
7. Yang J, Katagiri D, Mao S, et al., 2016, Inkjet Printing Based Assembly of Thermoresponsive Core–shell Polymer Microcapsules for Controlled Drug Release. J Mater Chem B, 4:4156-63. DOI 10.1039/c6tb00424e. 
8. Chen YW, Shen YF, Ho CC, et al., 2018, Osteogenic and Angiogenic Potentials of the Cell-laden Hydrogel/Mussel-inspired Calcium Silicate Complex Hierarchical Porous Scaffold Fabricated by 3D Bioprinting. Mater Sci Eng C Mater Biol Appl, 91:679-87. DOI 10.1016/j.msec.2018.06.005. 
9. Xu T, Zhao W, Zhu JM, et al., 2013, Complex Heterogeneous Tissue Constructs Containing Multiple Cell Types Prepared by Inkjet Printing Technology. Biomaterials, 34:130-9. DOI 10.1016/j.biomaterials.2012.09.035. 
10. Arai K, Iwanaga S, Toda H, et al., 2011, Three-dimensional Inkjet Biofabrication Based on Designed Images. Biofabrication, 3:034113. DOI 10.1088/1758- 5082/3/3/034113. 
11. Huang KH, Chen YW, Wang CY, et al., 2018, Enhanced Capability of BMP-2-loaded Mesoporous Calcium Silicate Scaffolds to Induce Odontogenic Differentiation of Human Dental Pulp Cells. J Endod, 44:1677-85. DOI 10.1016/j. joen.2018.08.008. 
12. Hench LL, 1991, Bioceramics: From Concept to Clinic. J Am Ceram Soc, 74:1457-510. 
13. Wang W, Yeung KWK, 2017, Bone Grafts and Biomaterials Substitutes for Bone Defect Repair: A Review. Bioact Mater, 2:224-47. DOI 10.1016/j.bioactmat.2017.05.007. 
14. Chen YW, Hsu TT, Wang K, et al., 2016, Preparation of the Fast Setting and Degrading Ca-Si-Mg Cement with both Odontogenesis and Angiogenesis Differentiation of Human Periodontal Ligament Cells. Mater Sci Eng C Mater Biol Appl, 60:374-83. DOI 10.1016/j.msec.2015.11.064. 
15. Wu YH, Chiu YC, Lin YH, et al., 2019, 3D-printed Bioactive Calcium Silicate/Poly-ε-caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration. Int J Mol Sci, 20:942. DOI 10.3390/ijms20040942. 
16. Ma H, Feng C, Chang J, et al., 2018, 3D-printed Bioceramic Scaffolds: From Bone Tissue Engineering to Tumor Therapy. Acta Biomater, 79:37-59. DOI 10.1016/j.actbio.2018.08.026. 
17. Carlisle EM, 1970, Silicon: A Possible Factor in Bone Calcification. Science, 167:279-80. 
18. Shie MY, Ding SJ, Chang HC, 2011, The Role of Silicon in Osteoblast-like Cell Proliferation and Apoptosis. Acta Biomater, 7:2604-14. DOI 10.1016/j.actbio.2011.02.023. 
19. Chen YW, Ho CC, Huang TH, et al., 2016, The Ionic Products from Mineral Trioxide Aggregate-induced Odontogenic Differentiation of Dental Pulp Cells via Activation of the Wnt/β-catenin Signaling Pathway. J Endod, 42:1062-9. DOI 10.1016/j.joen.2016.04.019. 
20. Ge L, Li QL, Huang Y, et al., 2014, Polydopamine-coated Paper-stack Nanofibrous Membranes Enhancing Adipose Stem Cells’ Adhesion and Osteogenic Differentiation. J Mater Chem B, 2:6917-23. DOI 10.1039/c4tb00570h. 
21. Ku SH, Ryu J, Hong SK, et al., 2010, General Functionalization Route for Cell Adhesion on Non-wetting Surfaces. Biomaterials, 31:2535-41. DOI 10.1016/j. biomaterials.2009.12.020. 
22. Lee YB, Shin YM, Kim EM, et al., 2016, Mussel Adhesive Protein Inspired Coatings on Temperature-responsive Hydrogels for Cell Sheet Engineering. J Mater Chem B, 4:6012-22. DOI 10.1039/c6tb01057a. 
23. Rim NG, Kim SJ, Shin YM, et al., 2012, Mussel-inspired Surface Modification of Poly(L-lactide) Electrospun Fibers for Modulation of Osteogenic Differentiation of Human Mesenchymal Stem Cells. Colloids Surf B, 91:189-97. DOI 10.1016/j.colsurfb.2011.10.057. 
24. Sun X, Cheng L, Zhao J, et al., 2014, bFGF-grafted Electrospun Fibrous Scaffolds via Poly(dopamine) for Skin Wound Healing. J Mater Chem B, 2:3636-45. DOI 10.1039/ c3tb21814g. 
25. Lee H, Rho J, Messersmith PB, 2009, Facile Conjugation of Biomolecules Onto Surfaces via Mussel Adhesive Protein Inspired Coatings. Adv Mater, 21:431-4. DOI 10.1002/ adma.200801222. 
26. Jo S, Kang SM, Park SA, et al., 2013, Enhanced Adhesion of Preosteoblasts Inside 3D PCL Scaffolds by Polydopamine Coating and Mineralization. Macromol Biosci, 13:1389-95. DOI 10.1002/mabi.201300203. 
27. Chen X, Wu Y, Ranjan VD, et al., 2018, Three-dimensional Electrical Conductive Scaffold from Biomaterial-based Carbon Microfiber Sponge with Bioinspired Coating for Cell Proliferation and Differentiation. Carbon, 134:174-82. DOI 10.1016/j.carbon.2018.03.064. 
28. Shie MY, Chiang WH, Chen IWP, et al., 2017, Synergistic Acceleration in the Osteogenic and Angiogenic Differentiation of Human Mesenchymal Stem Cells by Calcium Silicate-graphene Composites. Mater Sci Eng C Mater Biol Appl, 73:726-35. DOI 10.1016/j.msec.2019.01.059. 
29. Liu H, Li W, Luo B, et al., 2017, Icariin Immobilized Electrospinning Poly(l-lactide) Fibrous Membranes via Polydopamine Adhesive Coating with Enhanced Cytocompatibility and Osteogenic Activity. Mater Sci Eng C Mater Biol Appl, 79:399-409. DOI 10.1016/j. msec.2017.05.077. 
30. Shie MY, Ding SJ, 2013, Integrin Binding and MAPK Signal Pathways in Primary Cell Responses to Surface Chemistry of Calcium Silicate Cements. Biomaterials, 34:6589-606. DOI 10.1016/j.biomaterials.2013.05.075. 
31. Steeves AJ, Atwal A, Schock SC, et al., 2016, Evaluation of the Direct Effects of Poly(dopamine) on the in vitro Response of Human Osteoblastic Cells. J Mater Chem B, 4:3145-56. DOI 10.1039/c5tb02510a. 
32. Liu H, Li W, Wen W, et al., 2017, Mechanical Properties and Osteogenic Activity of Poly(l-lactide) Fibrous Membrane Synergistically Enhanced by Chitosan Nanofibers and Polydopamine Layer. Mater Sci Eng C Mater Biol Appl, 81:280-90. DOI 10.1016/j.msec.2017.08.010. 
33. Lee YB, Shin YM, Lee JH, et al., 2012, Polydopamine-mediated Immobilization of Multiple Bioactive Molecules for the Development of Functional Vascular Graft Materials. Biomaterials, 33:8343-52. DOI10.1016/j.biomaterials.2012.08.011. 
34. Tsai CH, Hung CH, Kuo CN, et al., 2019, Improved Bioactivity of 3D Printed Porous Titanium Alloy Scaffold with Chitosan/Magnesium-calcium Silicate Composite for Orthopaedic Applications. Materials, 12:203. DOI 10.3390/ ma12020203. 
35. Kao CT, Chen YJ, Ng HY, et al., 2018, Surface Modification of Calcium Silicate via Mussel-inspired Polydopamine and Effective Adsorption of Extracellular Matrix to Promote Osteogenesis Differentiation for Bone Tissue Engineering. Materials, 11:1664. DOI 10.3390/ma11091664. 
36. Wu C, Zhou YZ, Lin C, et al., 2012, Strontium-containing Mesoporous Bioactive Glass Scaffolds with Improved Osteogenic/Cementogenic Differentiation of Periodontal Ligament Cells for Periodontal Tissue Engineering. Acta Biomater, 8:3805-15. DOI 10.1016/j.actbio.2012.06.023. 
37. Chen YW, Yeh CH, Shie MY, 2015, Stimulatory Effects of the Fast Setting and Degradable Ca–Si–Mg Cement on Both Cementogenesis and Angiogenesis Differentiation of Human Periodontal Ligament Cells. J Mater Chem B, 3:7099-108. DOI 10.1039/c5tb00713e. 
38. Chou MY, Kao CT, Hung CJ, et al., 2014, Role of the p38 Pathway in Calcium Silicate Cement-induced Cell Viability and Angiogenesis-related Proteins of Human Dental Pulp Cell in vitro. J Endod, 40:818-24. DOI 10.1016/j. joen.2013.09.041. 
39. Cheng CH, Chen YW, Lee KX, et al., 2019, Development of Mussel-inspired 3D-printed Poly (lactic acid) Scaffold Grafted with Bone Morphogenetic Protein-2 for Stimulating Osteogenesis. J Mater Sci Mater Med, 30:78. DOI 10.1007/ s10856-019-6279-x. 
40. Poh CK, Shi Z, Lim TY, et al., 2010, The Effect of VEGF Functionalization of Titanium on Endothelial Cells In vitro. Biomaterials, 31:1578-85. DOI 10.1016/j. biomaterials.2009.11.042.

Share
Back to top
International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here
Accept