AccScience Publishing / IJB / Volume 10 / Issue 1 / DOI: 10.36922/ijb.1342
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RESEARCH ARTICLE

An in vitro model of meningioma constructed by 3D coaxial bioprinting

Peng Chen1,2 ,  Yongan Jiang1,2 ,  Hengyi Fan1 ,  Jianwei Chen3 ,  Raorao Yuan1 ,  Fan Xu4 ,  Shiqi Cheng1* ,  Yan Zhang1*
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1 Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China
2 School of Medicine, Nanchang University, Nanchang 330006, Jiangxi, China
3 Bio-intelligent Manufacturing and Living Matter Bioprinting Center, Research Institute of Tsinghua University in Shenzhen, Tsinghua University, Shenzhen 518000, China
4 Department of Neurosurgery, Nanchang People’s Hospital, Nanchang County 330200, Jiangxi, China
IJB 2024, 10(1), 1342 https://doi.org/10.36922/ijb.1342
Submitted: 20 July 2023 | Accepted: 7 August 2023 | Published: 1 September 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Meningioma, a type of brain tumor, has a high incidence rate and requires a comprehensive treatment approach due to its invasive nature and varying malignancy. In vitro models for studying the molecular mechanisms of malignant meningiomas and drug screening are urgently needed. However, two-dimensional (2D) culture of meningioma cells has limitations and challenges. Three-dimensional (3D) printing technology can provide a more realistic in vitro research platform for tumor research. In this study, 3D coaxial bioprinting was used to fabricate an in vitro 3D model of meningioma that faithfully recapitulates the biological characteristics and microenvironment of this malignancy. The bioprinted construct features a fibrous core-shell structure that allows cell clustering and fusion into cell fibers, ultimately forming a complex 3D structure resembling meningioma. Our findings suggest that the 3D model of meningioma generated by coaxial bioprinting closely resembles the in vivo morphology and growth pattern. Compared with 2D culture, 3D culture conditions better simulated the tumor microenvironment and exhibited higher invasiveness and tumorigenicity. Comparative analysis of biomarker expression further demonstrated that 3D culture provides a more accurate reflection of the biological characteristics of tumors. Our research affirms that microtissue models produced by 3D coaxial bioprinting can replicate the in vivo environment of cancer cells, producing survival conditions that are nearly realistic and more conducive to the restoration of cancer cell traits in vitro. 

Keywords
Bioprinting
Coaxial
Meningioma
Self-assembling
In vitro model
Funding
This research was supported by the National Natural Science Foundation (Grant Nos. 82260378 and 82260388), Natural Science Foundation of Jiangxi Province (Grant Nos. 20201BBG71007 and 20212BAB216053), and Traditional Chinese Medicine Foundation of Jiangxi Province (Grant No. 2022Z017).
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Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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