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ORIGINAL RESEARCH ARTICLE

Therapeutic prospective for anti-cervical cancer activity of Silybum marianum leaf aqueous extract-based vanadium nanoparticles

Mohammad Mahdi Zangeneh1 Akram Zangeneh1*
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1 Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
CP 2023, 5(3), 2574
Submitted: 29 April 2023 | Accepted: 2 July 2023 | Published: 21 July 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Silybum marianum is a plant with many remedial properties and may help prevent the cancer spread. Studies in this field show that this plant can reduce the growth of cancer cells. Probably, Silybum marianum will improve the effectiveness of chemotherapy. Also, the side effects of the recent treatments may be reduced by using this plant. The FDA has not confirmed Silybum marianum for the cancer treatment, but it may be effective in the treatment of these cancers: prostate, breast, cervical, blood, small intestine, and skin. Researching formulation of metallic nanoparticles by medicinal plants is the research priority of all countries. In the current experiment, we synthesize the vanadium nanoparticles by the watery extract of the Silybum marianum aerial parts. The characterization was conducted by FE-SEM, TEM, XRD, FT-IR, EDS, and UV-Vis. The DPPH inhibition efficacy was assessed by the DPPH examination, while the MTT assay was used to evaluate anti-cervical cancer (against LM-MEL-41, HT-3, Ca Ski, DoTc2 4510, SiHa, and C-33 A cells) and cytotoxicity efficacy of vanadium NPs. In XRD, the signals at 2 theta values of 25.13, 27.77, 44.94, 49.52, 66.28, and 70.57 belong to the planes of (202), (103), (401), (205), (406), and (125) respectively. Based on the findings of FE-SEM, the NPs are formed with the morphology of spherical with an aggregation. In FT-IR, the peaks at 416 and 551 cm-1 can be assigned to V-O-V and V-O bonds. The EDS analysis confirms the vanadium presence by the signals at 5.45 (VKβ), 4.98 (VKα), and 0.53 (VLα). The other signals below 0.5 KeV verify the appearance of carbon and oxygen in the green synthetic vanadium nanoparticles. The V nanoparticles IC50 was 126, 157, 165, 125, 132, and 197 µg/mL against LM-MEL-41, HT-3, DoTc2 4510, C-33 A, SiHa, and Ca Ski cervical cancer cells, respectively.

Keywords
Vanadium nanoparticles
Antioxidant
Anti-cervical cancer
Silybum marianum
Funding
Nil.
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Conflict of interest
There is no competing interest.
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