Wastewater Treatment by the Cyanobacterium Species: Synechococcus elongatus as Biosorption Material for Pb, Cr and Ni Heavy Metals

This research studies the effect of carbon dioxide on microalgae during the biological treatment of wastewater. Synechococcus elongatus was cultivated to eliminate heavy metal pollutants (lead, chromium and nickel) at different concentrations (0.5, 1.0, 2.0, 5.0 and 10.0ppm) under rich-CO2 environment. 50 ml of microalgae cultural medium were bubbled with 2 liters/ minute of CO2 gas, three times a week for two weeks. The results demonstrate that the effectiveness of microalgae growth and pollutant removal is directly impacted by CO2 gas dosages, as quickly and efficiently cell adaption in comparison to control groups. The highest pollutant removal was 100% (during day 10 of cultivation) with the presence of CO2 and 99.93% (during day 14 of cultivation) without the presence of CO2 for Pb at 0.5 ppm.
Andrson, A., Kartthikeyan, A., Ramachandran, S. and T.R. Praveenkumar (2021). Lowest emission sustainable aviation biofuels as the potential replacement for the Jet-A fuels. Aircraft Engineering and Aerospace Technology,93: 502-507. https://doi.org/10.1108/AEAT-07-2020-0135
Abed, I.J., Al-Hussieny, A.A., Kamel, R.F. and A. Jawad(2014). Environmental and identification study of algae present in three drinking water plants located on Tigris river in Baghdad. International Journal of Advanced Research, 2: 895-900.
Abed, I.J., Abdulhasan, G.A. and L.I. Moushib (2019). Molecular and immunological methods to confirm toxiginicity (microcystin production) of Westiellopsis prolifica isolated from Tigris River – Iraq. Baghdad Science Journal, 16: 0978-0978. https://doi.org/10.21123/ bsj.2019.16.4(Suppl.).0978.
Abed, I.J., Abdulhasan, G.A. and A.M. Najem (2018). Genotype versus phenotype to determine the definitive identification of the genera Chlorella beijerinck, 1890 (Chlorellaceae) and Coelastrella chodat, 1922
(Scendesmaseae). Bulletin of the Iraq Natural History Museum, 15: 101-111. https://doi.org/10.26842/ binhm.7.2018.15.1.0101
AL-Mashhadani, M.K.H., Wilkinson, S.J. and W.B. Zimmerman (2015). Airlift bioreactor for biological applications with microbubble mediated transport processes. Chemical Engineering Science, 137: 243-253. https://doi.org/10.1016/j.ces.2015.06.032
Anushree, Kumar, S. and C. Sharma (2017). Synthesis,characterization and catalytic performance of ZnO–CeO2 nanoparticles in wet oxidation of wastewater containing chlorinated compounds. Applied Nanoscience, 7: 567-575. https://doi.org/10.1007/s13204-017-0596-5
Biesta-Peters, E.G., Reij, M.W., Joosten, H., Gorris, L.G.M. and M.H. Zwietering (2010). Comparison of two optical- density-based methods and a plate count method for estimation of growth parameters of Bacillus cereus. Applied and Environmental Microbiology, 76(5): 1399-1405.
Cheng, L., Zhang, L., Chen, H. and C. Gao (2006). Carbon dioxide removal from air by microalgae cultured in a membrane-photobioreactor. Separation and Purification Technology, 50: 324-329. https://doi.org/10.1016/j. seppur.2005.12.006
Chiu, S.-Y., Kao, C.-Y., Tsai, M.-T., Ong, S.-C., Chen, C.-H. and C.-S. Lin (2009). Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresource Technology, 100: 833-838. https:// doi.org/10.1016/j.biortech.2008.06.061
Danouche, M., El Ghachtouli, N. and H. El Arroussi (2021). Phycoremediation mechanisms of heavy metals using living green microalgae: Physicochemical and molecular approaches for enhancing selectivity and removal capacity. Heliyon, 7(7): e07609. https://doi.org/10.1016/j. heliyon.2021.e07609
Fu, F. and Q. Wang (2011). Removal of heavy metal ions from wastewaters: A review. Journal of Environmental Management, 92: 407-418. https://doi.org/10.1016/j. jenvman.2010.11.011
Ganesan, R., Manigandan, S., Samuel, M.S., Shanmuganathan, R., Brindhadevi, K., Lan Chi, N.T., Duc, P.A. and A. Pugazhendhi (2020). A review on prospective production of biofuel from microalgae. Biotechnology Reports, 27: e00509. https://doi.org/10.1016/j.btre.2020.e00509
Hassan, H.A., Al-Mashhadani, M.K.H., Abed, K.M. and I.J. Abed (2021). The optimal utilization of lighting factor in the flat photobioreactor for biological applications. Materials Today: Proceedings, 42: 2534-2540. https://doi. org/10.1016/j.matpr.2020.12.575
Kim, D.-H., Yoon, J.-J., Kim, S.-H. and J.-H. Park (2021). Effect of conductive material for overcoming inhibitory conditions derived from red algae-based substrate on biohydrogen production. Fuel, 285: 119059. https://doi. org/10.1016/j.fuel.2020.119059
Kothari, V., Patadia, M. and N. Trivedi (2011). Microwave sterilized media supports better microbial growth than autoclaved media. Research in Biotechnology, 25(3): 63-72.
Mahdi R.S., Al-Mashhadani M.K.H. and I.J. Abed (2021). Pre-dissolved inorganic carbon (DIC) for cultivation Chlorella sorokiniana MH923013, Coelastrella MH923011 and Coelastrella MH923012. IOP Conf. Series: Materials Science and Engineering, 1076: 012025.
Makki, M.J., Al-Mashhadani, M.K.H. and S.K. Al-Dawery(2023). Removal of ranitidine using chlorella Sorokiniana MH923013. Iraqi Journal of Chemical and Petroleum Engineering, 24(2): 31-39.
Mitra, S., Chakraborty, A.J., Tareq, A.M., Emran, T.B., Nainu, F., Khusro, A., Idris, A.M., Khandaker, M.U., Osman, H., Alhumaydhi, F.A. and J. Simal-Gandara (2022). Impact of heavy metals on the environment and human health: Novel therapeutic insights to counter the toxicity. Journal of King Saud University - Science, 34: 101865. https://doi. org/10.1016/j.jksus.2022.101865
Mohsenpour, S.F. and N. Willoughby (2016). Effect of CO2 aeration on cultivation of microalgae in luminescent photobioreactors. Biomass and Bioenergy, 85: 168-177. https://doi.org/10.1016/j.biombioe.2015.12.002
Mojiri, A., Baharlooeian, M., Kazeroon, R.A., Farraji,H. and Z. Lou (2021). Removal of pharmaceutical micropollutants with integrated biochar and marine microalgae. Microorganisms, 9(1): 4. https://doi. org/10.3390/microorganisms9010004.
Rai, M.P. and S. Gupta (2017). Effect of media composition and light supply on biomass, lipid content and FAME profile for quality biofuel production from Scenedesmus abundans. Energy Conversion and Management, 141: 85-92. https://doi.org/10.1016/j.enconman.2016.05.018
Sekar, M., Mathimani, T., Alagumalai, A., Chi, N.T.L., Duc, P.A., Bhatia, S.K., Brindhadevi, K. and A. Pugazhendhi
(2021). A review on the pyrolysis of algal biomass for biochar and bio-oil – Bottlenecks and scope. Fuel, 283:119190.https://doi.org/10.1016/j.fuel.2020.119190
Spain, O., Plöhn, M. and C. Funk (2021). The cell wall of green microalgae and its role in heavy metal removal. Physiologia Plantarum, 173: 526-535. https://doi. org/10.1111/ppl.13405.
Wang, J. and C. Chen (2009). Biosorbents for heavy metals removal and their future. Biotechnology Advances, 27: 195-226. https://doi.org/10.1016/j.biotechadv.2008.11.002
Xiong, J.-Q., Govindwar, S., Kurade, M.B., Paeng, K.-J., Roh, H.-S., Khan, M.A. and B.-H. Jeon,. (2019). Toxicity of sulfamethazine and sulfamethoxazole and their removal by a green microalga, Scenedesmus obliquus. Chemosphere, 218: 551-558. https://doi.org/10.1016/j. chemosphere.2018.11.146.
Yan, C., Qu, Z., Wang, J., Cao, L. and Q. Han (2022). Microalgal bioremediation of heavy metal pollution in water: Recent advances, challenges, and prospects. Chemosphere, 286: 131870. https://doi.org/10.1016/j. chemosphere.2021.131870
Zhu, Y., Rong, J., Zhang, T., Xu, J., Dai, Y. and F. Qiu (2018). Controlled and facile synthesis of a self-assembled enzyme–inorganic catalyst based on flexible metal-coated fiber for an excellent removal of synthetic pollutants from aqueous environment. Applied Nanoscience, 8: 1139-1148. https://doi.org/10.1007/s13204-018-0791-z