AccScience Publishing / AJWEP / Volume 16 / Issue 3 / DOI: 10.3233/AJW190031
RESEARCH ARTICLE

Assessing Genotoxic and Cytotoxic Effects in Bivalves  Influenced by Marine Pollution in Bahrain, Arabian Gulf

Abdulla A.R. Abdulla,1 Humood A. Naser1* Ghaida J. Ayyad1
Show Less
1 Department of Biology, College of Science, University of Bahrain, P.O. Box 32038, Kingdom of Bahrain
AJWEP 2019, 16(3), 35–42; https://doi.org/10.3233/AJW190031
Submitted: 9 November 2018 | Revised: 6 May 2019 | Accepted: 6 May 2019 | Published: 19 July 2019
© 2019 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC-by the license) ( https://creativecommons.org/licenses/by-nc/4.0/ )
Abstract

The Arabian Gulf is increasingly subjected to habitat alteration due to dredging, reclamation activities,  and pollution from various anthropogenic sources, including industrial and domestic effluents and oil spills.  These activities can affect the naturally stressed marine ecosystems. Genotoxicity and cytotoxicity biomarkers  are widely recognized as an important tool to assess the biological response of organisms exposed to a variety  of contaminants. This study investigated the genotoxic and cytotoxic effects in bivalves influenced by marine  pollution in Bahrain. The results revealed that high percentage of abnormal cells (45.57%) was associated with a  shallow bay influenced by elevated levels of nutrients from a major sewage station. Characterization of genotoxic  and cytotoxic biomarkers can be adopted to monitor coastal and marine areas influenced by anthropogenic landbased activities in the Arabian Gulf

Keywords
Genotoxicity
cytotoxicity
marine pollution
Arabian Gulf.
Conflict of interest
The authors declare they have no competing interests.
References

Aanand, S., Purushothaman, C., Pal, A. and K. Rajendran  (2010). Toxicological studies on the effect of copper, lead  and zinc on selected enzymes in the adductor muscle and  intestinal diverticula of green mussel Perna viridis. Indian  Journal of Marine Sciences, 39(2): 299-302. 

Barsiene, J., Andreikenaite, L., Garnaga, G. and A. Rybakovas  (2008). Genotoxic and cytotoxic effects in the bivalve  mollusks Macoma balthica and Mytilus edulis from Baltic  Sea. Ekologia, 54(1): 44-45. 

Barsiene, J., Rybakovas, A., Garhaga, G. and L. Andreikenaite  (2012). Environmental genotoxicity and cytotoxicity  studies in mussels before and after an oil spill at the marine  oil terminal in the Baltic Sea. Environmental Monitoring  and Assessment, 184: 2067-2078.

Bouilly, K., Bonnard, M., Gagnaire, B., Renault, T. and S.  Lapegue (2007). Impact of diuron on aneuploidy and  hemocyte parameters in Pacific Oyster, Crassostrea gigas.  Archives of Environmental Contamination and Toxicology,  22: 219-223. 

Bussel, C., Mahlmann, L., Kroeckel, S., Schroeder, J. and C.  Schulz (2013). The effect of high ortho-phosphate water  levels on growth, feed intake, nutrient utilization and  health status of juvenile turbot (Psetta maxima) reared  in intensive recirculating aquaculture systems (RAS).  Aquacultural Engineering, 57: 63-70. 

Chen, W., Tong, H. and H. Lin (2012). Effects of nitrate on  nitrite toxicity to Microcystis aeruginosa. Marine Pollution  Bulletin, 64: 1106-1111.

Hernandez, A., Gasper, M., Macho, G. and E. Vazquez (2014).  Age and growth of the sword razor clams Ensis arcuatus  in the Ria de Pontevedra (NW Spain): Influence of the  environmental parameters. Journal of Sea Research, 85: 59-72. 

Holmes, R. and B. Goldberg (2009). Computational analysis  of mammalian lactate dehydrogenase: Human, mouse,  opossum and platypus LDHs. Computational Biology &  Chemistry, 33(5): 379-385. 

Hylland, K., Tollefsen, K., Ruus, A., Jonsson, G., Sundt, R.,  Sanni, S., Utvik, T., Johnsen, S., Nilssen, I., Pinturier,  L., Balk, L., Barisiene, J., Marigonmez, I., Feist, S. and  F. Borseth (2008). Water column monitoring near oil  installation in the North Sea. Marine Pollution Bulletin,  56: 414-429.

Janiva, B., Alexandras, R., Galina, G. and A. Laura (2012).  Environmental genotoxicity and cytotoxicity studies in  mussels before and after an oil spill at the marine oil  terminal in Baltic Sea. Environmental Monitoring and  Assessment, 184: 2067-2078.

Jones, D. (1986). A field guide to the sea shores of Kuwait  and the Arabian Gulf. University of Kuwait, Kuwait.

Knepp, G. and G. Arkin (1973). Ammonia toxicity levels  and nitrate tolerance of channel catfish. The Progressive  Fish-Culturist, 35(4): 221-224

Ladeira, C., Viegas, S., Carolino, E., Gomes, M. and M.  Brito (2012). Genotoxicity Biomarkers: Application in  Histopathology Laboratories. Biomarkers. Khan, T. INTEC  Publications. 

Lee, A. and K. Lee (2011). The enzyme activities of opine  and lactate dehydrogenase in the gills, mantle, foot and  adductor of the hard clam Meretrix lusoria. Journal of  Marine Science and Technology, 19(4): 361-367. 

Leitao, A., Al-Shaikh, I., Hassan, H. and R. Hamadou (2017).  First genotoxicity assessment of marine environment  in Qatar using the local Pearl oyster Pinctada radiata.  Regional Studies in Marine Science, 11: 23-31. 

Li, H., Zhang, C., Han, X. and X. Shi (2015). Changes in  concentrations at oxygen, dissolved nitrogen, phosphate,  and silicate in the southern Yellow Sea 1980-2012: Sources  and seaward gradients. Estuarine, Coastal and Shelf  Science, 163: 44-55.

Li, P., Diao, X., Zhang, Y., Xie, Y., Yang, F., Zhou, H., Han,  Q., Wang, F., Cheng, H. and H. Wang (2015). Polycyclic  aromatic hydrocarbons in surface sediment from Yangpu  Bay, China: Distribution, sources and risk assessment.  Marine Pollution Bulletin, 99: 312-319.

Lowry, O.H., Rosebrough, N.J., Farr, A.L. and R.J. Randall  (1951). Protein measurement with the Folin phenol  reagents. Journal of Biological Chemistry, 193: 265-275.

Naser, H. (2011). Effects of reclamation on macrobenthic  assemblages in the coastline of the Arabian Gulf: A  microcosm experimental approach. Marine Pollution  Bulletin, 62: 520-524.

Naser, H. (2013). Assessment and management of heavy  metal pollution in the marine environment of the Arabian  Gulf: A review. Marine Pollution Bulletin, 72: 6-13. 

Nunes, B., Travasso, R., Goncalves, F. and B. Castro (2015).  Biochemical and physiological modifications of Sardina  pilchardus: Spatial and temporal patterns as a baseline  for biomonitoring studies. Frontiers in Environmental  Science, 3: 1-14. 

Rode, J. (2008). Apoptosis, Cytotoxicity and Cell Proliferation,  Fourth Edition. Roche Diagnostics GmbH. 

Sabharwal, R. (2015). Emergence of Micronuclei as a  Genomic Biomarker: A Review. Indian Journal of Medical  & Pediatric Oncology, 36(4): 212-218. 

Samarco, P., Lolian, S., Warby, R., Bouldin, J., Subra, W.  and S. Porter (2013). Distribution and concentrations of  petroleum hydrocarbons associated with the BP/Deepwater  Horizon Oil spill, Gulf of Mexico. Marine Pollution  Bulletin, 73(1): 129-143.

Shah, S. (2012). Importance of Genotoxicity and S2A  Guidelines for Genotoxicity Testing for Pharmaceuticals.  Journal of Pharmacy and Biological Sciences, 1(2): 43-54. 

Shanmugam, P., Neelamani, S., Ahn, Y., Philip, L. and G.  Hong (2006). Assessment of the levels of coastal marine  pollution of Chennai city, Southern India. Water Resources  Management, 21(7): 1187-1206.

Simboura, N. and A. Zenetos (2002). Benthic indicators to use  in ecological quality classification of Mediterranean soft  bottom marine ecosystems, including a new biotic index.  Mediterranean Marine Science, 3(2): 77-111. 

Sraj, L., Almeida, M., Swearer, S., Kolev, S. and I. McKelvie  (2014). Analytical challenges and advantages of using  flow-based methodologies for ammonia determination  in estuarine and marine waters. Trends in Analytical  Chemistry, 59: 83-92.

Sulaiman, R., Ismail, Z., Othman, S., Ramli, A. and S. Shirazi  (2014). A comparative study of trends of nitrate, chloride  and phosphate concentration levels in selected urban  rivers. Measurements, 55: 74-81.

Tobias, N. (2008). Marine Pollution: New Research, First  Edition. Nova Science Publishers, New York.

Tsuda, R. (1997). Turbidity and Suspended Solids  Concentration in Coastal Areas. Oceanographical Society  of Japan, 12: 51-62. 

Vaughan, G., Al-Mansoori, N. and J. Burt (2018). The  Arabian Gulf. In: Sheppard, C. (ed.), World Seas: An  Environmental Evaluation, second edition. Elsevier  Science, Amsterdam, NL.

Wardrop, P., Shimeta, J., Nugegoda, D., Morrison, P.,  Miranda, A., Tang, M. and B. Clarke (2016). Chemical  Pollutants Sorbed to Ingested Microbeads from Personal  Care Products Accumulate in Fish. Environmental Science  & Technology, 50(7): 4037-4044.

Wright, S. (2013). The Physical Impacts of Microplastics on  Marine Organisms: A Review. Environmental Pollution,  178: 483-492.

Share
Back to top
Asian Journal of Water, Environment and Pollution, Electronic ISSN: 1875-8568 Print ISSN: 0972-9860, Published by AccScience Publishing