Bioremediation of Herbicide Atrazine by Fungal sp. Aspergillus alliaceus Strain JAV1 Isolated from Paddy Field Soil in Vellore
The herbicide atrazine (2-Chloro-4-ethylamino-6-isopropylamino-s-triazine) is widely used on crops such as maize, sugarcane, corn, pineapple and sorghum. Although atrazine is an effective herbicide, the persistence of this herbicide leads to contamination of soil, groundwater and surface water. This work presents the laboratory studies on the degradation of atrazine. Atrazine degrading fungal strain JAV1 was isolated from paddy field. The molecular characterization based on 18S rRNA sequence revealed the strain JAV1 as Aspergillus alliaceus. The strain was able to degrade atrazine at the concentration of 1500 mgL-1 and their complete degradation was seen on sixth day of incubation by strain JAV1. The results were confirmed with the help of sophisticated instruments such as high performance liquid chromatography (HPLC), gas chromatography mass spectroscopy (GC-MS) and fourier transform infrared spectroscopy (FTIR).
Chen, H., Bramanti, E., Longo, I., Onor, M. and C. Ferrari (2011). Oxidative decomposition of atrazine in water in the presence of hydrogen peroxide using an innovation microwave photochemical reactor. J. Hazard. Mater, 186: 1808-1815.
Christin, M.S., Menard, L., Gendron, A.D., Tuby, S., Cyr, D., Marcogliese, D.J., Smith, L.R. and M. Fournier (2004). Effects of agricultural pesticides on the immune system of Xenopus laevis and Rana pipiens. Aquat. Toxic., 6: 33-43.
Doruk, A.Y. and N. Kolankaya (2012). Biodegradation of atrazine by a selected white-rot fungal strain in optimized conditions. Eur. Int. J. Sci. Technol., 1(2): 1-10.
Fazlurrahman, B.M., Pandey, J., Suri, C.R. and R.K. Jain (2009). Isolation and characterization of an atrazine-degrading Rhodococcus sp. strain MB-P1 from contaminated soil. Letters in Applied Microbiology, 49: 721-729.
Feria-Reyes, R., Medina-Armenta, P., Teutli-Leon, M., García-Jiménez, M.G. and Gonzalez (2011). A new approach for atrazine desorption, extraction and detection from a clay-silty soil sample. Am. J. Analyt. Chem., 2: 63-68.
Gebendinger, N. and Radosevich, M. (1999). Inhibition of atrazine degradation by cyanazine and exogenous nitrogen in bacterial isolate M91-3. Appl. Microbiol. Biotechnol., 51: 375-381.
Hickey, W.J., Fuster, D.J. and R.T. Lamar (1994). Transformation of atrazine in soil by Phanerochaete chrysosporium. Soil. Biochem., 26: 1665-1671.
Khan, S.U. and W.J. Saidak (1981). Residues of atrazine and metabolites after prolonged usage. Weed Res., 21: 9-12.
Mougin, C., Laugero, C., Asther, M., Dubroca, J., Frasse, P. and A. Marcel (1994). Biotransformation of the herbicide atrazine by the white rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol, 60: 705-708.
Murphy, M., Hecker, B.M., Coady, K.K., Tompsett, A.R., Jones, P.D., Preez, L.H.D., Everson, G.J., Solomon, K.R., Carr, J.A., Smith, E.E., Kendall, R.J., Kraak, V.D. and J.P. Giesy (2006). Atrazine concentrations, gonodal gross morphology and histology in frogs collected in Michigan agricultural areas. Aquat. Toxicol., 76: 230-245.
Ojo, O.A. (2007). Microbial utilization of the hydrocarbon components of atrazine in a tropical soil environment southwest, Nigeria. Afr. J. Infect. Dis., 1(1): 25-29.
Peter, L., Gajendiran, A., Mani, D., Nagaraj, S. and J. Abraham (2014). Mineralization of malathion by Fusarium oxysporum strain JASA1 isolated from sugarcane fields. Environmental Progress & Sustainable Energy, 34(1): DOI 10.1002/ep11970.
Schiavon, M. (1988). Studies of the leaching of atrazine of its chlorinated derivatives, and of hydroxyatrazine from soil using 14C ring-labeled compounds under outdoor conditions. Ecotoxicol. Environ. Saf., 15: 46-54.
Shenoy, K. (2012). Environmentally Realistic Exposure to the Herbicide Atrazine Alters Some Sexually Selected Traits in Male Guppies. PLoS ONE, 7(2): e30611.
Solomon, R.D.J., Kumar, A. and V.S. Santhi (2013). Atrazine biodegradation efficiency, metabolite detection and trzD gene expression by enrichment bacterial cultures from agricultural soil. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 14(12): 1162-1172.
Struthers, J.K., Jayachandranand, K. and T.B. Moorman (1998). Biodegradation of Atrazine by Agrobacterium radiobacter J14a and Use of This Strain in Bioremediation of Sediments and Surface Water. American Chemical Society, 42(2): 432-436.
Swain, D.J. (1981). Atrazine dissipation in irrigated sorghum cropping in southern New South Wales. Weed Res., 21: 13-21.
Topp, E., Zhu, H., Nour, S.M., Houot, S., Lewis, M. and D. Cuppels (2000). Characterization of an atrazinedegradation Pseudaminobacter sp. isolated from Canadian and French agricultural soils. Appl Environ Microb, 66: 2773-2782.
Umar, A.F., Tahir, F., Michael, J., Larkin, Oyawoye, O.M., Musa, B.L., Yerima, M.B. and E.B. Agbo (2012). AtzABC Catabolic Gene Probe from Novel Atrazine-Degrading Rhodococcus Strain Isolated from a Nigerian Agricultural Soil. Advances in Microbiology, 2: 593-597.
Wang, J., Zhu, L., Wang, Qi, Wang, J. and Hui Xie (2014). Isolation and Characterization of Atrazine Mineralizing Bacillus subtilis Strain HB-6. PLoS ONE, 9(9): e107270. doi:10.1371/journal.pone.0107270.
Zhang, Y. (2009). The research of immobilized atrazine degrading bacteria degrading characteristics. Int. Conf. Environ. Sci. Inf. Appl. Tech., 677-680.